The roles of DNA topoisomerase II during the cell cycle

DNA TOPOISOMERASE POISONS AS ANTITUMOR DRUGS

1989 • 1,799 citations

DNA TOPOISOMERASES

1985 • 1,524 citations

Topoisomerase II is a structural component of mitotic chromosome scaffolds.

1985 • 751 citations

DNA topoisomerase II is required at the time of mitosis in yeast

1985 • 747 citations

The cyclosome, a large complex containing cyclin-selective ubiquitin ligase activity, targets cyclins for destruction at the end of mitosis.

1995 • 745 citations

Transcription generates positively and negatively supercoiled domains in the template

1988 • 742 citations

DNA topoisomerase II is required for condensation and separation of mitotic chromosomes in S. pombe

1987 • 691 citations

DNA topoisomerase II mutant of Saccharomyces cerevisiae: topoisomerase II is required for segregation of daughter molecules at the termination of DNA replication.

1984 • 685 citations

Anaphase is initiated by proteolysis rather than by the inactivation of maturation-promoting factor

1993 • 569 citations

Calcium Rises Abruptly and Briefly Throughout the Cell at the Onset of Anaphase

1986 • 541 citations

Metaphase chromosome structure

1986 • 538 citations

Mitotic forces control a cell-cycle checkpoint

1995 • 535 citations

Localization of topoisomerase II in mitotic chromosomes.

1985 • 501 citations

In situ localization of DNA topoisomerase II, a major polypeptide component of the Drosophila nuclear matrix fraction.

1985 • 472 citations

Isolation of type I and II DNA topoisomerase mutants from fission yeast: single and double mutants show different phenotypes in cell growth and chromatin organization.

1984 • 444 citations

Topoisomerase II: A specific marker for cell proliferation.

1986 • 434 citations

Need for DNA topoisomerase activity as a swivel for DNA replication for transcription of ribosomal RNA

1987 • 426 citations

Differential expression of DNA topoisomerases I and II during the eukaryotic cell cycle.

1988 • 415 citations

Biochemical and pharmacological properties of p170 and p180 forms of topoisomerase II

1989 • 409 citations

Sgs1: A eukaryotic homolog of E. coil RecQ that interacts with topoisomerase II in vivo and is required for faithful chromosome segregation

1995 • 402 citations

Cloning and sequencing of cDNA encoding human DNA topoisomerase II and localization of the gene to chromosome region 17q21-22.

1988 • 391 citations

Chromosome assembly in vitro: Topoisomerase II is required for condensation

1991 • 382 citations

Meiotic spindle assembly in Drosophila females: behavior of nonexchange chromosomes and the effects of mutations in the nod kinesin-like protein.

1992 • 377 citations

Disassembly of the nucleus in mitotic extracts: Membrane vesicularization, lamin disassembly, and chromosome condensation are independent processes

1987 • 349 citations

Kinetochore chemistry is sensitive to tension and may link mitotic forces to a cell cycle checkpoint.

1995 • 344 citations

Antitumor bisdioxopiperazines inhibit yeast DNAtopoisomerase II by trapping the enzyme in the form of a closed proteinclamp.

1994 • 339 citations

Regulation of the cell cycle by the cdk2 protein kinase in cultured human fibroblasts.

1993 • 325 citations

A topoisomerase II-dependent G2 cycle checkpoint in mammalian cells

1994 • 315 citations

The organisation of chromatin loops: characterization of a scaffold attachment site

1986 • 308 citations

DNA Topoisomerase II Must Act at Mitosis To Prevent Nondisjunction and Chromosome Breakage

1989 • 302 citations

Mitotic spindle pulls but fails to separate chromosomes in type II DNA topoisomerase mutants: uncoordinated mitosis

1986 • 292 citations

Mutations in the gene coding for Escherichia coli DNA topoisomerase I affect transcription and transposition.

1981 • 284 citations

Purification of topoisomerase II from amsacrine-resistant P388 leukemia cells. Evidence for two forms of the enzyme.

1987 • 282 citations

Okadaic acid, a potent inhibitor of type 1 and type 2A protein phosphatases, activates cdc2/H1 kinase and transiently induces a premature mitosis-like state in BHK21 cells.

1990 • 280 citations

Transcription-dependent DNA supercoiling in yeast DNA topoisomerase mutants

1988 • 277 citations

Different patterns of gene expression of topoisomerase II isoforms in differentiated tissues during murine development

1992 • 253 citations

ScII: an abundant chromosome scaffold protein is a member of a family of putative ATPases with an unusual predicted tertiary structure.

1994 • 252 citations

Characterization and immunological identification of cDNA clones encoding two human DNA topoisomerase II isozymes.

1989 • 250 citations

Proliferation-dependent regulation of DNA topoisomerase II in cultured human cells.

1988 • 244 citations

Isolation of cDNA clones encoding the β isozyme of human DNA topoisomerase II and localisation of the gene to chromosome 3p24

1992 • 237 citations

Topoisomerase II does not play a scaffolding role in the organization of mitotic chromosomes assembled in Xenopus egg extracts.

1993 • 235 citations

Inhibition of p34cdc2 kinase activity by etoposide or irradiation as a mechanism of G2 arrest in Chinese hamster ovary cells.

1990 • 222 citations

Cell cycle-dependent cytotoxicity, G2/M phase arrest, and disruption of p34cdc2/cyclin B1 activity induced by doxorubicin in synchronized P388 cells.

1996 • 206 citations

Mitotic chromatin condensation in vitro using somatic cell extracts and nuclei with variable levels of endogenous topoisomerase II.

1990 • 181 citations

Chromatin structure of transcriptionally competent and repressed genes.

1990 • 178 citations

Multiple chromosomal populations of topoisomerase II detected in vivo by time-lapse, three-dimensional wide-field microscopy

1993 • 176 citations

Yeast DNA topoisomerase II is encoded by a single-copy, essential gene

1984 • 175 citations

Degradation of the proto-oncogene product p39mos is not necessary for cyclin proteolysis and exit from meiotic metaphase: requirement for a Ca(2+)-calmodulin dependent event.

1991 • 174 citations

Function of DNA topoisomerases as replication swivels in Saccharomyces cerevisiae

1989 • 174 citations

Inhibitors of DNA topoisomerase II prevent chromatid separation in mammalian cells but do not prevent exit from mitosis.

1991 • 174 citations

Segregation of recombined chromosomes in meiosis I requires DNA topoisomerase II

1990 • 172 citations

Casein kinase II phosphorylates the eukaryote-specific C-terminal domain of topoisomerase II in vivo.

1992 • 170 citations

Newly replicated DNA is associated with DNA topoisomerase II in cultured rat prostatic adenocarcinoma cells

1986 • 168 citations

Relationship between topoisomerase II level and chemosensitivity in human tumor cell lines.

1991 • 165 citations

DNA topoisomerase II alpha is the major chromosome protein recognized by the mitotic phosphoprotein antibody MPM-2.

1993 • 162 citations

Inhibition of DNA topoisomerase II by ICRF-193 induces polyploidization by uncoupling chromosome dynamics from other cell cycle events.

1994 • 157 citations

DNA topoisomerase I is essential in Drosophila melanogaster.

1993 • 144 citations

Topoisomerase-specific drug sensitivity in relation to cell cycle progression.

1987 • 144 citations

Specific phosphorylation of proteins in pore complex-laminae from the sponge Geodia cydonium by the homologous aggregation factor and phorbol ester. Role of protein kinase C in the phosphorylation of DNA topoisomerase II.

1987 • 140 citations

Multidrug sensitivity phenotype of human lung cancer cells associated with topoisomerase II expression.

1992 • 138 citations

Identification of Saccharomyces cerevisiae mutants deficient in DNA topoisomerase I activity.

1984 • 137 citations

Inhibition of calf thymus type II DNA topoisomerase by poly(ADP-ribosylation).

1985 • 126 citations

Phosphorylation of DNA topoisomerase II in vivo and in total homogenates of Drosophila Kc cells. The role of casein kinase II.

1988 • 126 citations

Assembly of spaced chromatin involvement of ATP and DNA topoisomerase activity.

1988 • 125 citations

The Cell Cycle-Coupled Expression of Topoisomerase IIα during S Phase Is Regulated by mRNA Stability and Is Disrupted by Heat Shock or Ionizing Radiation

1996 • 121 citations

Cell cycle progression and chromosome segregation in mammalian cells cultured in the presence of the topoisomerase II inhibitors ICRF-187 [(+)-1,2-bis(3,5-dioxopiperazinyl-1-yl)propane; ADR-529] and ICRF-159 (Razoxane).

1994 • 121 citations

In vivo phosphorylation of the 170-kDa form of eukaryotic DNA topoisomerase II

1989 • 112 citations

Monoclonal antibodies to human DNA topoisomerase I and the two isoforms of DNA topoisomerase II: 170- and 180-kDa isozymes

1992 • 110 citations

Effects of cytosine arabinoside, daunomycin, mithramycin, azacytidine, adriamycin, and camptothecin on mammalian cell cycle traverse.

1972 • 106 citations

A postprophase topoisomerase II-dependent chromatid core separation step in the formation of metaphase chromosomes.

1995 • 104 citations

Cell cycle stage-dependent variations in drug-induced topoisomerase II mediated DNA cleavage and cytotoxicity

1987 • 103 citations

Cloning and characterization of the 5'-flanking region of the human topoisomerase II alpha gene.

1992 • 99 citations

The 180-kDa isoform of topoisomerase II is localized in the nucleolus and belongs to the structural elements of the nucleolar remnant

1992 • 98 citations

Growth state- and cell cycle-dependent fluctuation in the expression of two forms of DNA topoisomerase II and possible specific modification of the higher molecular weight form in the M phase.

1994 • 96 citations

Functional dissection of the phosphorylated termini of fission yeast DNA topoisomerase II.

1992 • 94 citations

DNA topoisomerase II and casein kinase II associate in a molecular complex that is catalytically active.

1993 • 94 citations

The SMC family: Novel motor proteins for chromosome condensation?

1994 • 91 citations

Differences between normal and ras-transformed NIH-3T3 cells in expression of the 170kD and 180kD forms of topoisomerase II.

1990 • 91 citations

The topoisomerase II inhibitor VM-26 induces marked changes in histone H1 kinase activity, histones H1 and H3 phosphorylation, and chromosome condensation in G2 phase and mitotic BHK cells.

1990 • 90 citations

Consequences of topoisomerase II inhibition in early embryogenesis of Drosophila revealed by in vivo confocal laser scanning microscopy

1993 • 89 citations

Precise localization of the alpha-globin gene cluster within one of the 20- to 300-kilobase DNA fragments released by cleavage of chicken chromosomal DNA at topoisomerase II sites in vivo: evidence that the fragments are DNA loops or domains.

1991 • 86 citations

Serine 1524 is a major site of phosphorylation on human topoisomerase II alpha protein in vivo and is a substrate for casein kinase II in vitro.

1994 • 84 citations

The complete nucleotide sequence of the structural gene TOP2 of yeast DNA topoisomerase II.

1986 • 80 citations

Cell Cycle Phase-specific Phosphorylation of Human Topoisomerase IIα

1995 • 75 citations

Concentration-dependent differences in the mechanisms by which caffeine potentiates etoposide cytotoxicity in HeLa cells.

1994 • 73 citations

Molecular cloning of partial cDNAs for rat DNA topoisomerase II isoforms and their differential expression in brain development.

1993 • 70 citations

Purification and characterization of type II DNA topoisomerase from mouse FM3A cells: phosphorylation of topoisomerase II and modification of its activity

1990 • 70 citations

Growth-related elevations of DNA topoisomerase II levels found in Dunning R3327 rat prostatic adenocarcinomas.

1987 • 64 citations

Nature and distribution of chromosomal intertwinings in Saccharomyces cerevisiae.

1994 • 64 citations

Epidermal growth factor-induced topoisomerase(s)

1983 • 64 citations

Phosphorylation of DNA topoisomerase II in a human tumor cell line.

1991 • 63 citations

Topoisomerase II plays an essential role as a swivelase in the late stage of SV40 chromosome replication in vitro.

1992 • 62 citations

Identification of the nature of modification that causes the shift of DNA topoisomerase II beta to apparent higher molecular weight forms in the M phase.

1994 • 61 citations

Investigation of the mechanism of higher order chromatin fragmentation observed in drug-induced apoptosis.

1995 • 61 citations

Isolation and characterization of a human cDNA clone encoding a novel DNA topoisomerase II homologue from HeLa cells

1990 • 59 citations

Growth state and cell cycle dependent phosphorylation of DNA topoisomerase II in Swiss 3T3 cells

1992 • 58 citations

Protein kinase C modulates the catalytic activity of topoisomerase II by enhancing the rate of ATP hydrolysis: evidence for a common mechanism of regulation by phosphorylation

1993 • 56 citations

DNA topoisomerase II is required for formation of mitotic chromosomes in Chinese hamster ovary cells: studies using the inhibitor 4'-demethylepipodophyllotoxin 9-(4,6-O-thenylidene-.beta.-D-glucopyranoside)

1990 • 54 citations

Effect of cell proliferation and chromatin conformation on intercalator-induced, protein-associated DNA cleavage in human brain tumor cells and human fibroblasts.

1987 • 51 citations

Cell-cycle-dependent phosphorylation and activity of Chinese-hamster ovary topoisomerase II

1993 • 48 citations

The arrangement of H5 molecules in extended and condensed chicken erythrocyte chromatin.

1985 • 48 citations

Effect of casein kinase II-mediated phosphorylation on the catalytic cycle of topoisomerase II. Regulation of enzyme activity by enhancement of ATP hydrolysis.

1992 • 47 citations

Isolation and characterization of the gene encoding Drosophila DNA topoisomerase II.

1986 • 47 citations

ADP-Ribosylation of Nonhistone Proteins in HeLa Cells: Modification of DNA Topoisomerase II

1993 • 46 citations

Topoisomerase II Forms Multimers In Vitro: Effects of Metals, β-Glycerophosphate, and Phosphorylation of Its C-Terminal Domain

1994 • 44 citations

Expression of the Drosophila type II topoisomerase is developmentally regulated

1988 • 43 citations

Analysis of the inhibitory effects of VP-16-213 (etoposide) and podophyllotoxin on thymidine transport and metabolism in Ehrlich ascites tumor cells in vitro.

1984 • 38 citations

Selective displacement of nuclear proteins by antitumor drugs having affinity for nucleic acids.

1989 • 36 citations

Phosphorylation of the .alpha.- and .beta.-Isoforms of DNA Topoisomerase II Is Qualitatively Different in Interphase and Mitosis in Chinese Hamster Ovary Cells

1994 • 36 citations

Inhibitors of Topoisomerase II Delay Progress through Mitosis and Induce a Doubling of the DNA Content in CHO Cells

1995 • 35 citations

Checkpoint controls that couple mitosis to completion of DNA replication

1992 • 32 citations

Effects of antileukemia agents on nuclear matrix-bound DNA replication in CCRF-CEM leukemia cells.

1988 • 31 citations

Topoisomerase II alpha and beta in human tumor cells grown in vitro and in vivo.

1992 • 31 citations

Purification and characterization of a nuclear DNA-binding factor complex containing topoisomerase II and chromosome scaffold protein 2.

1993 • 26 citations

Cell-Cycle-Regulated Localization of Tyrosine and Threonine Phosphoepitopes at the Kinetochores of Mitotic Chromosomes

1995 • 25 citations

Adenovirus E1A-induced apoptosis elicits a steep decrease in the topoisomerase II alpha level during the latent phase.

1995 • 22 citations

Increment of DNA topoisomerases in chemically and virally transformed cells

1988 • 22 citations

Expression of drug resistance-associated mdr-1, GST π, and topoisomerase II genes during cell cycle traverse

1995 • 22 citations

Effects of VM26, a specific inhibitor of type II DNA topoisomerase, on SV40 chromatin replicationin vitro

1988 • 17 citations

Altered formation of DNA in human cells treated with inhibitors of DNA topoisomerase II (etoposide and teniposide).

1989 • 14 citations

Role of Topoisomerase II in the Structural and Functional Evolution of Mitogen-Stimulated Lymphocyte Nuclei

1994 • 11 citations

Association of topoisomerases I and II with the chromatin in SV40-infected monkey cells

1991 • 8 citations

ANALYSIS OF PHOSPHORYLATION LEVEL OF DNA TOPOISOMERASE-II-ALPHA AND TOPOISOMERASE-II-BETA IN HUMAN HELA-CELLS

1995 • 3 citations