Can sequence determine function?

The Complete Genome Sequence of Escherichia coli K-12

1997 • 7,325 citations

Genome Sequence of the Nematode C. elegans : A Platform for Investigating Biology

1998 • 3,683 citations

The complete genome sequence of the Gram-positive bacterium Bacillus subtilis

1997 • 3,613 citations

Genome sequence of the nematode C-elegans: A platform for investigating biology

1998 • 3,265 citations

Assigning protein functions by comparative genome analysis: Protein phylogenetic profiles

1999 • 1,556 citations

Evolving Genes and Proteins

1965 • 1,314 citations

A Proficient Enzyme

1995 • 859 citations

Evolving Genes and Proteins

1965 • 802 citations

Catalytic promiscuity and the evolution of new enzymatic activities

1999 • 734 citations

Phylogenomics: Improving Functional Predictions for Uncharacterized Genes by Evolutionary Analysis

1998 • 632 citations

The Structure of ClpP at 2.3 Å Resolution Suggests a Model for ATP-Dependent Proteolysis

1997 • 603 citations

On the Evolution of Biochemical Syntheses

1945 • 595 citations

Divergent Evolution of Enzymatic Function: Mechanistically Diverse Superfamilies and Functionally Distinct Suprafamilies

2001 • 535 citations

An explanation for rapid enzyme-catalyzed proton abstraction from carbon acids: importance of late transition states in concerted mechanisms

1993 • 370 citations

Evolutionary Divergence of Substrate Specificity within the Chymotrypsin-like Serine Protease Fold

1997 • 351 citations

Structural Evidence for Evolution of the β/α Barrel Scaffold by Gene Duplication and Fusion

2000 • 301 citations

The Enolase Superfamily: A General Strategy for Enzyme-Catalyzed Abstraction of the α-Protons of Carboxylic Acids

1996 • 297 citations

Understanding Enzyme Superfamilies

1997 • 280 citations

Catalytic Plasticity of Fatty Acid Modification Enzymes Underlying Chemical Diversity of Plant Lipids

1998 • 259 citations

How far divergent evolution goes in proteins

1998 • 236 citations

Electrostatic stress in catalysis: Structure and mechanism of the enzyme orotidine monophosphate decarboxylase

2000 • 208 citations

Metabolism of Ferulic Acid to Vanillin

1998 • 199 citations

Directed evolution of new catalytic activity using the α/β-barrel scaffold

2000 • 191 citations

Crystal structure of enoyl-coenzyme A (CoA) hydratase at 2.5 angstroms resolution: a spiral fold defines the CoA-binding pocket.

1996 • 189 citations

Anatomy of a proficient enzyme: The structure of orotidine 5′-monophosphate decarboxylase in the presence and absence of a potential transition state analog

2000 • 175 citations

Directed evolution of a (βα) ₈ -barrel enzyme to catalyze related reactions in two different metabolic pathways

2000 • 172 citations

The crystal structure and mechanism of orotidine 5′-monophosphate decarboxylase

2000 • 159 citations

Structure of 4-Chlorobenzoyl Coenzyme A Dehalogenase Determined to 1.8 Å Resolution: An Enzyme Catalyst Generated via Adaptive Mutation^,

1996 • 155 citations

Discovering New Enzymes and Metabolic Pathways: Conversion of Succinate to Propionate by Escherichia coli

2000 • 146 citations

A Functionally Diverse Enzyme Superfamily that Abstracts the α Protons of Carboxylic Acids

1995 • 138 citations

Molecular characterization of the cai operon necessary for carnitine metabolism in Escherichia coii

1994 • 116 citations

The crystal structure of enoyl-CoA hydratase complexed with octanoyl-CoA reveals the structural adaptations required for binding of a long chain fatty acid-CoA molecule 1 1Edited by D. Rees

1998 • 115 citations

Unexpected Divergence of Enzyme Function and Sequence: “N-Acylamino Acid Racemase” Is o-Succinylbenzoate Synthase

1999 • 112 citations

Three-dimensional structure of the bifunctional enzyme phosphoribosylanthranilate isomerase: Indoleglycerolphosphate synthase from Escherichia coli refined at 2.0 Å resolution

1992 • 110 citations

Mechanistically diverse enzyme superfamilies: the importance of chemistry in the evolution of catalysis

1998 • 94 citations

Structural Basis for the Catalytic Mechanism of a Proficient Enzyme: Orotidine 5‘-Monophosphate Decarboxylase^,

2000 • 94 citations

The crystal structure of dienoyl-CoA isomerase at 1.5 å resolution reveals the importance of aspartate and glutamate sidechains for catalysis

1998 • 82 citations

New Reactions in the Crotonase Superfamily: Structure of Methylmalonyl CoA Decarboxylase from Escherichia coli^,

2000 • 82 citations

Molecular evolution of the histidine biosynthetic pathway

1995 • 74 citations

Menaquinone (vitamin K2) biosynthesis: nucleotide sequence and expression of the menB gene from Escherichia coli

1992 • 72 citations

Enoyl-CoA Hydratase and Isomerase form a Superfamily with a Common Active-Site Glutamate Residue

1995 • 72 citations

Interchange of Catalytic Activity within the 2-Enoyl-Coenzyme A Hydratase/Isomerase Superfamily Based on a Common Active Site Template

1999 • 69 citations

Enoyl-CoA Hydratase and Isomerase form a Superfamily with a Common Active-Site Glutamate Residue

1995 • 67 citations

Evolution of Enzymatic Activity in the Enolase Superfamily: Structure of o-Succinylbenzoate Synthase from Escherichia coli in Complex with Mg²⁺ and o-Succinylbenzoate^,

2000 • 64 citations

2-Ketocyclohexanecarboxyl Coenzyme A Hydrolase, the Ring Cleavage Enzyme Required for Anaerobic Benzoate Degradation by Rhodopseudomonas palustris

1998 • 60 citations

Role of Glutamate 144 and Glutamate 164 in the Catalytic Mechanism of Enoyl-CoA Hydratase

1999 • 53 citations

Primary Structure and Tissue-specific Expression of Human β-Hydroxyisobutyryl-coenzyme A Hydrolase

1996 • 48 citations

Purification and properties of thermostable N-acylamino acid racemase from Amycolatopsis sp. TS-1-60

1995 • 38 citations

Enoyl-Coenzyme A Hydratase-Catalyzed Exchange of the .alpha.-Protons of Coenzyme A Thiol Esters: A Model for an Enolized Intermediate in the Enzyme-Catalyzed Elimination?

1994 • 38 citations

Isotope effects on the crotonase reaction

1989 • 33 citations

Evolution of Enzymatic Activities in the Enolase Superfamily: Identification of a “New” General Acid Catalyst in the Active Site of <scp>d</scp>-Galactonate Dehydratase from Escherichia coli

1999 • 32 citations

The Chemistry of the Reaction Determines the Invariant Amino Acids during the Evolution and Divergence of Orotidine 5′-Monophosphate Decarboxylase

2000 • 30 citations

Activity of Yeast Orotidine-5′-phosphate Decarboxylase in the Absence of Metals

1999 • 27 citations

Paralogous histidine biosynthetic genes: evolutionary analysis of the Saccharomyces cerevisiae HIS6 and HIS7 genes

1997 • 27 citations

No Metal Cofactor in Orotidine 5′-Monophosphate Decarboxylase

1999 • 27 citations

Menaquinone (vitamin K2) biosynthesis: overexpression, purification, and properties of o-succinylbenzoyl-coenzyme A synthetase from Escherichia coli

1996 • 26 citations

3-Hexulosephosphate synthase from Methylomonas aminofaciens 77a Purification, properties and kinetics

1978 • 24 citations

Evolution of Enzymatic Activities: Multiple Pathways for Generating and Partitioning a Common Enolic Intermediate by Glucarate Dehydratase from Pseudomonas putida

1996 • 21 citations

Evolution of Enzymatic Activities in the Enolase Superfamily: Partitioning of Reactive Intermediates by (D)-Glucarate Dehydratase from Pseudomonas putida

1998 • 16 citations

Anatomy of a proficient enzyme: The structure of orotidine 5'-monophosphate decarboxylase in the presence and absence of a potential transition state analog

2000 • 5 citations

Purification and properties of thermostable N-acylamino acid racemase from Amycolatopsis sp. TS-1-60

1995 • 3 citations

The Chemistry of the Reaction Determines the Invariant Amino Acids during the Evolution and Divergence of Orotidine 5′-Monophosphate Decarboxylase

2000 • 2 citations