Enhanced detection, maintenance, and differentiation of primitive human hematopoietic cells in cultures containing murine fibroblasts engineered to produce human steel factor, interleukin-3, and granulocyte colony-stimulating factor

Tight control of gene expression in mammalian cells by tetracycline-responsive promoters.

1992 • 4,885 citations

Transforming growth factor beta 1 null mutation in mice causes excessive inflammatory response and early death.

1993 • 1,941 citations

A safe packaging line for gene transfer: separating viral genes on two different plasmids

1988 • 1,118 citations

Establishment of mouse cell lines which constitutively secrete large quantities of interleukin 2, 3, 4 or 5, using modified cDNA expression vectors

1988 • 1,086 citations

Functional characterization of individual human hematopoietic stem cells cultured at limiting dilution on supportive marrow stromal layers.

1990 • 697 citations

Long-term culture of human bone marrow cells.

1980 • 624 citations

Differential expression of homeobox genes in functionally distinct CD34+ subpopulations of human bone marrow cells.

1994 • 529 citations

Self-renewal of primitive human hematopoietic cells (long-term-culture-initiating cells) in vitro and their expansion in defined medium.

1996 • 393 citations

Differential cytokine effects on primitive (CD34+CD38-) human hematopoietic cells: novel responses to Flt3-ligand and thrombopoietin.

1996 • 360 citations

A stromal cell line from myeloid long-term bone marrow cultures can support myelopoiesis and B lymphopoiesis.

1987 • 345 citations

Functionally distinct human marrow stromal cell lines immortalized by transduction with the human papilloma virus E6/E7 genes

1995 • 340 citations

Support of human hematopoiesis in long-term bone marrow cultures by murine stromal cells selectively expressing the membrane-bound and secreted forms of the human homolog of the steel gene product, stem cell factor.

1992 • 306 citations

Enzymatic treatment of long-term human marrow cultures reveals the preferential location of primitive hemopoietic progenitors in the adherent layer

1983 • 296 citations

Mechanisms that regulate the cell cycle status of very primitive hematopoietic cells in long-term human marrow cultures. II. Analysis of positive and negative regulators produced by stromal cells within the adherent layer

1991 • 292 citations

Large-scale expansion of human stem and progenitor cells from bone marrow mononuclear cells in continuous perfusion cultures

1993 • 280 citations

Differential regulation of primitive human hematopoietic cells in long- term cultures maintained on genetically engineered murine stromal cells

1991 • 261 citations

Direct contact between human primitive hematopoietic progenitors and bone marrow stroma is not required for long-term in vitro hematopoiesis

1992 • 245 citations

Long-term erythropoiesis from constant numbers of CD34+ cells in serum-free cultures initiated with highly purified progenitor cells from human bone marrow.

1992 • 231 citations

Dominant Positive and Negative Selection Using a Hygromycin Phosphotransferase-Thymidine Kinase Fusion Gene

1991 • 167 citations

Characterization and purification of a primitive hematopoietic cell type in adult mouse marrow capable of lymphomyeloid differentiation in long-term marrow "switch" cultures

1995 • 131 citations

Expansion of Hematopoietic Progenitor Cell Populations in Stirred Suspension Bioreactors of Normal Human Bone Marrow Cells

1994 • 131 citations

Clonal Hematopoiesis Demonstrated by X-Linked DNA Polymorphisms after Allogeneic Bone Marrow Transplantation

1989 • 121 citations

Expansion in vitro of retrovirally marked totipotent hematopoietic stem cells

1990 • 100 citations

Maintenance of high levels of pluripotent hematopoietic stem cells in vitro: effect of stromal cells and c-kit

1993 • 92 citations

Methodology of long-term culture of human hemopoietic cells

1991 • 91 citations

Alternative mechanisms with and without steel factor support primitive human hematopoiesis

1993 • 90 citations

Identification and mutation of primary and secondary proteolytic cleavage sites in murine stem cell factor cDNA yields biologically active, cell-associated protein.

1994 • 89 citations

Retroviral gene transfer to primitive normal and leukemic hematopoietic cells using clinically applicable procedures.

1992 • 73 citations

Human granulocytes generated in continuous bone marrow culture are physiologically normal

1981 • 64 citations

A modified indicator gene for selection of retrotransposition events in mammalian cells.

1994 • 63 citations

A novel single-cell proliferation assay shows that long-term culture- initiating cell (LTC-IC) maintenance over time results from the extensive proliferation of a small fraction of LTC-IC

1995 • 61 citations

Partial characterization of a novel stromal cell-derived pre-B-cell growth factor active on normal and immortalized pre-B cells.

1988 • 58 citations

The effects of interleukin 6 and interleukin 3 on early hematopoietic events in long-term cultures of human marrow.

1991 • 47 citations

Differential effects of microenvironmentally presented interleukin 3 versus soluble growth factor on primitive human hematopoietic cells.

1991 • 37 citations

Very high concentrations of cytokines are required for the maximal stimulation in vitro of primitive human hematopoietic progenitors and are depleted most rapidly by subpopulations of cells that are highly enriched in their LTC-IC content

1995 • 4 citations