One-Day Ex Vivo Culture Allows Effective Gene Transfer Into Human Nonobese Diabetic/Severe Combined Immune-Deficient Repopulating Cells Using High-Titer Vesicular Stomatitis Virus G Protein Pseudotyped Retrovirus

Gene transfer by retrovirus vectors occurs only in cells that are actively replicating at the time of infection.

1990 • 1,465 citations

T Lymphocyte-Directed Gene Therapy for ADA ⁻ SCID: Initial Trial Results After 4 Years

1995 • 1,464 citations

Vesicular stomatitis virus G glycoprotein pseudotyped retroviral vectors: concentration to very high titer and efficient gene transfer into mammalian and nonmammalian cells.

1993 • 1,395 citations

Isolation of a candidate human hematopoietic stem-cell population.

1992 • 1,098 citations

Gene-marking to trace origin of relapse after autologous bone-marrow transplantation

1993 • 949 citations

A stable human-derived packaging cell line for production of high titer retrovirus/vesicular stomatitis virus G pseudotypes.

1996 • 925 citations

Purification of primitive human hematopoietic cells capable of repopulating immune-deficient mice

1997 • 879 citations

Gene Therapy in Peripheral Blood Lymphocytes and Bone Marrow for ADA ⁻ Immunodeficient Patients

1995 • 846 citations

Identification of primitive human hematopoietic cells capable of repopulating NOD/SCID mouse bone marrow: Implications for gene therapy

1996 • 795 citations

Functional characterization of individual human hematopoietic stem cells cultured at limiting dilution on supportive marrow stromal layers.

1990 • 697 citations

Engraftment of gene–modified umbilical cord blood cells in neonates with adenosine deaminase deficiency

1995 • 622 citations

Cell-surface receptors for gibbon ape leukemia virus and amphotropic murine retrovirus are inducible sodium-dependent phosphate symporters.

1994 • 586 citations

Retrovirally marked CD34-enriched peripheral blood and bone marrow cells contribute to long-term engraftment after autologous transplantation

1995 • 496 citations

Expansion in vitro of transplantable human cord blood stem cells demonstrated using a quantitative assay of their lympho-myeloid repopulating activity in nonobese diabetic– scid/scid mice

1997 • 495 citations

Engraftment of Immune-Deficient Mice with Human Hematopoietic Stem Cells

1988 • 471 citations

Quantitative Analysis Reveals Expansion of Human Hematopoietic Repopulating Cells After Short-term Ex Vivo Culture

1997 • 424 citations

Effects of retroviral vector design on expression of human adenosine deaminase in murine bone marrow transplant recipients engrafted with genetically modified cells.

1995 • 357 citations

Long-term repopulation of irradiated mice with limiting numbers of purified hematopoietic stem cells: in vivo expansion of stem cell phenotype but not function

1995 • 340 citations

Combination of interleukins 3 and 6 preserves stem cell function in culture and enhances retrovirus-mediated gene transfer into hematopoietic stem cells.

1989 • 316 citations

Pseudotype formation of murine leukemia virus with the G protein of vesicular stomatitis virus

1991 • 304 citations

Cell Cycle-Related Changes in Repopulating Capacity of Human Mobilized Peripheral Blood CD34+ Cells in Non-Obese Diabetic/Severe Combined Immune-Deficient Mice

1998 • 302 citations

Immature human cord blood progenitors engraft and proliferate to high levels in severe combined immunodeficient mice

1994 • 283 citations

Kinetic Evidence of the Regeneration of Multilineage Hematopoiesis From Primitive Cells in Normal Human Bone Marrow Transplanted Into Immunodeficient Mice

1997 • 247 citations

The level of mRNA encoding the amphotropic retrovirus receptor in mouse and human hematopoietic stem cells is low and correlates with the efficiency of retrovirus transduction.

1996 • 241 citations

Normal and leukemic SCID-repopulating cells (SRC) coexist in the bone marrow and peripheral blood from CML patients in chronic phase, whereas leukemic SRC are detected in blast crisis

1996 • 196 citations

Long-term expression of human adenosine deaminase in rhesus monkeys transplanted with retrovirus-infected bone-marrow cells.

1992 • 190 citations

Long-term in vivo expression of a murine adenosine deaminase gene in rhesus monkey hematopoietic cells of multiple lineages after retroviral mediated gene transfer into CD34+ bone marrow cells

1993 • 185 citations

Analysis of optimal conditions for retroviral-mediated transduction of primitive human hematopoietic cells

1995 • 182 citations

Long-term in vivo expression of a murine adenosine deaminase gene in rhesus monkey hematopoietic cells of multiple lineages after retroviral mediated gene transfer into CD34+ bone marrow cells

1993 • 168 citations

Efficient Retroviral-Mediated Gene Transfer to Human Cord Blood Stem Cells With In Vivo Repopulating Potential

1998 • 161 citations

Differential Maintenance of Primitive Human SCID-Repopulating Cells, Clonogenic Progenitors, and Long-Term Culture-Initiating Cells After Incubation on Human Bone Marrow Stromal Cells

1997 • 159 citations

Stem cell factor, interleukin-3, and interleukin-6 promote retroviral- mediated gene transfer into murine hematopoietic stem cells

1992 • 147 citations

Stromal support enhances cell-free retroviral vector transduction of human bone marrow long-term culture-initiating cells

1992 • 141 citations

Amplification of Sca-1+ Lin-WGA+ cells in serum-free cultures containing steel factor, interleukin-6, and erythropoietin with maintenance of cells with long-term in vivo reconstituting potential

1994 • 139 citations

Effects of transplantation on the primitive immunohematopoietic stem cell.

1990 • 136 citations

Expression of retroviral vectors containing the human multidrug resistance 1 cDNA in hematopoietic cells of transplanted mice

1995 • 110 citations

Stromal support enhances cell-free retroviral vector transduction of human bone marrow long-term culture-initiating cells

1992 • 93 citations

Rapid exit from G0/G1 phases of cell cycle in response to stem cell factor confers on umbilical cord blood CD34+ cells an enhanced ex vivo expansion potential.

1994 • 87 citations

Engraftment and Retroviral Marking of CD34+ and CD34+CD38− Human Hematopoietic Progenitors Assessed in Immune-Deficient Mice

1998 • 86 citations

High-efficiency gene transfer to human hematopoietic cells maintained in long-term marrow culture [see comments]

1989 • 83 citations

Gene transfer into normal human hematopoietic cells using in vitro and in vivo assays

1991 • 82 citations

Growth factors and stromal support generate very efficient retroviral transduction of peripheral blood CD34+ cells from Gaucher patients

1995 • 78 citations

Retroviral gene transfer to primitive normal and leukemic hematopoietic cells using clinically applicable procedures.

1992 • 73 citations

Retroviral vector-mediated gene transfer into primitive human hematopoietic progenitor cells: effects of mast cell growth factor (MGF) combined with other cytokines.

1992 • 72 citations

Pseudotyped retroviral vectors for studies of human gene therapy

1995 • 65 citations

Alternatives to stem cell renewal from a developmental viewpoint.

1997 • 49 citations

High-efficiency gene transfer and expression in normal human hematopoietic cells with retrovirus vectors

1988 • 45 citations

Highly effective CD34+ selection of granulocyte colony-stimulating factor-mobilized allogeneic peripheral blood progenitor cells [letter]

1996 • 15 citations

Gene-marking and haemopoietic stem-cell transplantation

1995 • 14 citations

Bone marrow purging with oligodeoxynucleotides.

1996 • 3 citations