Microtubule nucleating activity of centrosomes in cell-free extracts from Xenopus eggs: involvement of phosphorylation and accumulation of pericentriolar material

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

1976 • 200,127 citations

Universal control mechanism regulating onset of M-phase

1990 • 2,882 citations

Staurosporine, a potent inhibitor of phospholipidCa++dependent protein kinase

1986 • 2,445 citations

Cytoplasmic control of nuclear behavior during meiotic maturation of frog oocytes

1971 • 1,553 citations

K-252 compounds, novel and potent inhibitors of protein kinase C and cyclic nucleotide-dependent protein kinases

1987 • 947 citations

Purified maturation-promoting factor contains the product of a Xenopus homolog of the fission yeast cell cycle control gene cdc2+

1988 • 885 citations

Human cyclins A and B1 are differentially located in the cell and undergo cell cycle-dependent nuclear transport.

1991 • 858 citations

The Xenopus cdc2 protein is a component of MPF, a cytoplasmic regulator of mitosis

1988 • 829 citations

Microtubule assembly nucleated by isolated centrosomes

1984 • 775 citations

cdc2 protein kinase is complexed with both cyclin A and B: Evidence for proteolytic inactivation of MPF

1989 • 666 citations

Identification of γ-tubulin, a new member of the tubulin superfamily encoded by mipA gene of Aspergillus nidulans

1989 • 613 citations

Rapid stimulation by insulin of a serine/threonine kinase in 3T3-L1 adipocytes that phosphorylates microtubule-associated protein 2 in vitro.

1987 • 604 citations

γ-Tubulin is a highly conserved component of the centrosome

1991 • 566 citations

γ-tubulin is a component of the spindle pole body that is essential for microtubule function in Aspergillus nidulans

1990 • 558 citations

MPF from starfish oocytes at first meiotic metaphase is a heterodimer containing one molecule of cdc2 and one molecule of cyclin B.

1989 • 550 citations

γ-Tubulin is a centrosomal protein required for cell cycle-dependent microtubule nucleation

1992 • 447 citations

Regulation of microtubule dynamics by cdc2 protein kinase in cell-free extracts of Xenopus eggs

1990 • 446 citations

Real-time visualization of cell cycle-dependent changes in microtubule dynamics in cytoplasmic extracts

1990 • 444 citations

The cdc2 kinase is a nuclear protein that is essential for mitosis in mammalian cells

1989 • 439 citations

In vitro effects on microtubule dynamics of purified Xenopus M phase-activated MAP kinase

1991 • 422 citations

The pericentriolar material in Chinese hamster ovary cells nucleates microtubule formation

1977 • 416 citations

Microtubule Organizing Centers

1985 • 412 citations

Induction of nuclear envelope breakdown, chromosome condensation, and spindle formation in cell-free extracts.

1985 • 407 citations

γ-Tubulin is present in Drosophila melanogaster and homo sapiens and is associated with the centrosome

1991 • 405 citations

Insulin-stimulated microtubule-associated protein kinase is phosphorylated on tyrosine and threonine in vivo.

1988 • 404 citations

p13suc1 acts in the fission yeast cell division cycle as a component of the p34cdc2 protein kinase.

1987 • 387 citations

Microtubule‐associated‐protein (MAP) kinase activated by nerve growth factor and epidermal growth factor in PC12 cells

1990 • 386 citations

The A- and B-type cyclin associated cdc2 kinases in Xenopus turn on and off at different times in the cell cycle.

1990 • 377 citations

p34cdc2 is located in both nucleus and cytoplasm; part is centrosomally associated at G2/M and enters vesicles at anaphase.

1989 • 376 citations

A microtubule-associated protein from Xenopus eggs that specifically promotes assembly at the plus-end.

1987 • 338 citations

Centrosome development in early mouse embryos as defined by an autoantibody against pericentriolar material

1983 • 323 citations

THE CENTRIOLE CYCLE IN SYNCHRONIZED HELA CELLS

1968 • 316 citations

Activation at M-phase of a protein kinase encoded by a starfish homologue of the cell cycle control gene cdc2+

1988 • 314 citations

The fission yeast γ-tubulin is essential for mitosis and is localized at microtubule organizing centers

1991 • 294 citations

Structural and chemical characterization of isolated centrosomes

1987 • 292 citations

Xenopus M phase MAP kinase: isolation of its cDNA and activation by MPF.

1991 • 274 citations

Changes in protein phosphorylation accompanying maturation of Xenopus laevis oocytes

1977 • 269 citations

The Chromosome Cycle and the Centrosome Cycle in the Mitotic Cycle

1987 • 263 citations

Characterization of insulin-stimulated microtubule-associated protein kinase. Rapid isolation and stabilization of a novel serine/threonine kinase from 3T3-L1 cells.

1988 • 257 citations

Phosphoproteins are components of mitotic microtubule organizing centers.

1984 • 246 citations

Activation of a Ca2+-inhibitable protein kinase that phosphorylates microtubule-associated protein 2 in vitro by growth factors, phorbol esters, and serum in quiescent cultured human fibroblasts.

1988 • 240 citations

Mitotic spindle assembly by two different pathways in vitro.

1991 • 235 citations

Centrosomes and mitotic poles

1984 • 229 citations

Interconversion of metaphase and interphase microtubule arrays, as studied by the injection of centrosomes and nuclei into Xenopus eggs.

1984 • 225 citations

Cytoplasmic factor responsible for germinal vesicle breakdown and meiotic maturation in starfish oocyte

1976 • 221 citations

Distinct nuclear and spindle pole body populations of cyclin–cdc2 in fission yeast

1990 • 198 citations

Microtubule-nucleating activity of centrosomes in Chinese hamster ovary cells is independent of the centriole cycle but coupled to the mitotic cycle.

1981 • 191 citations

Microtubule assembly in cytoplasmic extracts of Xenopus oocytes and eggs.

1987 • 153 citations

Regulation of the microtubule nucleating activity of centrosomes in Xenopus egg extracts: role of cyclin A-associated protein kinase.

1992 • 135 citations

Mitogen‐activated‐protein‐kinase‐catalyzed phosphorylation of microtubule‐associated proteins, microtubule‐associated protein 2 and microtubule‐associated protein 4, induces an alteration in their function

1992 • 128 citations

A post-ribosomal supernatant from activated Xenopus eggs that displays post-translationally regulated oscillation of its cdc2+ mitotic kinase activity.

1989 • 126 citations

Phosphorylation changes associated with the early cell cycle in Xenopus eggs

1987 • 118 citations

Nucleation of microtubules from mitotic centrosomes is modulated by a phosphorylated epitope

1990 • 108 citations

Parthenogenesis in Xenopus eggs requires centrosomal integrity.

1990 • 103 citations

Effects of brain microtubule‐associated proteins on microtubule dynamics and the nucleating activity of centrosomes

1990 • 96 citations

In vitro microtubule-nucleating activity of spindle pole bodies in fission yeast Schizosaccharomyces pombe: cell cycle-dependent activation in xenopus cell-free extracts

1992 • 89 citations

Regulation of Metaphase by a Maturation‐Promoting Factor

1988 • 77 citations

Cyclins A and B associate with chromatin and the polar regions of spindles, respectively, and do not undergo complete degradation at anaphase in syncytial Drosophila embryos.

1992 • 71 citations

In vitro reactivation of spindle elongation in fission yeast nuc2 mutant cells.

1990 • 66 citations

Insertion of an R1 plasmid into the origin of replication of the E. coli chromosome: Random timing of replication of the hybrid chromosome

1986 • 61 citations

225‐Kilodalton phosphoprotein associated with mitotic centrosomes in sea urchin eggs

1989 • 52 citations

Human anticentriole autoantibody in patients with scleroderma and Raynaud's phenomenon

1983 • 41 citations

The centriolar complex isolated from starfish spermatozoa

1981 • 31 citations

The starfish egg mRNA responsible for meiosis reinitiation encodes cyclin

1990 • 29 citations

Localization of mitotic‐apparatus‐associated 51‐kD protein in unfertilized and fertilized sea urchin eggs

1988 • 28 citations

FURTHER OBSERVATIONS ON THE CLUSTERS OF GRANULAR MATERIAL AROUND THE CENTRIOLE IN THE SEA URCHIN EGG: CHANGES IN DISTRIBUTION DURING MITOSIS*

1980 • 24 citations