The fission yeast γ-tubulin is essential for mitosis and is localized at microtubule organizing centers

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2001 • 133,517 citations

Molecular Cloning. A Laboratory Manual

1983 • 27,328 citations

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

1985 • 15,222 citations

[12] One-step gene disruption in yeast

1983 • 3,302 citations

DNA cloning : a practical approach

1985 • 2,302 citations

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1964 • 1,816 citations

Vectors for selective expression of cloned DNAs by T7 RNA polymerase

1987 • 1,449 citations

Definition of individual components within the cytoskeleton of Trypanosoma brucei by a library of monoclonal antibodies

1989 • 650 citations

Identification of γ-tubulin, a new member of the tubulin superfamily encoded by mipA gene of Aspergillus nidulans

1989 • 613 citations

γ-tubulin is a component of the spindle pole body that is essential for microtubule function in Aspergillus nidulans

1990 • 558 citations

The use of cell division cycle mutants to investigate the control of microtubule distribution in the fission yeast Schizosaccharomyces pombe

1988 • 493 citations

The NDA3 gene of fission yeast encodes β-tubulin: A cold-sensitive nda3 mutation reversibly blocks spindle formation and chromosome movement in mitosis

1984 • 473 citations

Growth in cell length in the fission yeast Schizosaccharomyces pombe

1985 • 466 citations

Novel potential mitotic motor protein encoded by the fission yeast cut7+ gene

1990 • 366 citations

One-Step Gene Disruption in Yeast

1989 • 310 citations

Construction of a Schizosaccharomyces pombe gene bank in a yeast bacterial shuttle vector and its use to isolate genes by complementation

1982 • 242 citations

A temperature-sensitive mutation of the Schizosaccharomyces pombe gene nuc2+ that encodes a nuclear scaffold-like protein blocks spindle elongation in mitotic anaphase.

1988 • 220 citations

Identification of the pleiotropic cell division cycle gene NDA2 as one of two different α-tubulin genes in schizosaccharomyces pombe

1984 • 216 citations

Construction of aNotI restriction map of the fission yeastSchizosaccharomyces pombegenome

1989 • 204 citations

Distinct nuclear and spindle pole body populations of cyclin–cdc2 in fission yeast

1990 • 198 citations

High efficiency vectors for cosmid microcloning and genomic analysis

1989 • 190 citations

Interaction between cdc13+ and cdc2+ in the control of mitosis in fission yeast; dissociation of the G1 and G2 roles of the cdc2+ protein kinase.

1987 • 177 citations

Cold-sensitive nuclear division arrest mutants of the fission yeast Schizosaccharomyces pombe

1983 • 165 citations

The fission yeast cut1+ gene regulates spindle pole body duplication and has homology to the budding yeast ESP1 gene

1990 • 151 citations

Mitosis in the fission yeast Schizosaccharomyces pombe as revealed by freeze-substitution electron microscopy

1986 • 137 citations

Sequential alterations in the nuclear chromatin region during mitosis of the fission yeast Schizosa Ccharomyces Pombe-: Video fluorescence microscopy of synchronously growing wild-type and cold-sensitive cdc mutants by using a dna-binding fluorescent probe

1981 • 135 citations

Dynamics of cytoplasmic organelles in the cell cycle of the fission yeast Schizosaccharomyces pombe: three-dimensional reconstruction from serial sections

1989 • 134 citations

Differential expressions of essential and nonessential alpha-tubulin genes in Schizosaccharomyces pombe.

1986 • 94 citations

Visualization of chromosomes in mitotically arrested cells of the fission yeast Schizosaccharomyces pombe

1983 • 70 citations

In vitro reactivation of spindle elongation in fission yeast nuc2 mutant cells.

1990 • 66 citations

Intramitotic controls in the fission yeast Schizosaccharomyces pombe: the effect of cell size on spindle length and the timing of mitotic events.

1990 • 45 citations

The transition of cells of the fission yeast β-tubulin mutant nda3-311 as seen by freeze-substitution electron microscopy Requirement of functional tubulin for spindle pole body duplication

1990 • 37 citations