SSI1 Encodes a Novel Hsp70 of the Saccharomyces cerevisiae Endoplasmic Reticulum

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

Molecular Cloning. A Laboratory Manual

1983 • 27,328 citations

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

1989 • 8,705 citations

The codon adaptation index-a measure of directional synonymous codon usage bias, and its potential applications

1987 • 3,628 citations

Signal sequences

1985 • 2,179 citations

Studies on the transformation of intact yeast cells by the LiAc/SS‐DNA/PEG procedure

1995 • 2,001 citations

Current Protocols in Molecular Biology

1989 • 1,916 citations

Eukaryotic proteins expressed in Escherichia coli: An improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase

1991 • 1,779 citations

Elevated recombination rates in transcriptionally active DNA

1989 • 1,666 citations

Multifunctional yeast high-copy-number shuttle vectors

1992 • 1,656 citations

A rapid and simple method for preparation of RNA fromSaccharomyces cerevisiae

1990 • 1,340 citations

Structural Analysis of Substrate Binding by the Molecular Chaperone DnaK

1996 • 1,247 citations

The biology of heat shock proteins and molecular chaperones

1994 • 1,203 citations

Transcriptional induction of genes encoding endoplasmic reticulum resident proteins requires a transmembrane protein kinase

1993 • 1,197 citations

A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector

1987 • 1,181 citations

Three-dimensional structure of the ATPase fragment of a 70K heat-shock cognate protein

1990 • 1,035 citations

Successive action of DnaK, DnaJ and GroEL along the pathway of chaperone-mediated protein folding

1992 • 965 citations

Posttranslational association of immunoglobulin heavy chain binding protein with nascent heavy chains in nonsecreting and secreting hybridomas.

1986 • 896 citations

Expression of wild-type and mutant forms of influenza hemagglutinin: The role of folding in intracellular transport

1986 • 879 citations

Escherichia coli DnaJ and GrpE heat shock proteins jointly stimulate ATPase activity of DnaK.

1991 • 835 citations

A transmembrane protein with a cdc2+CDC28-related kinase activity is required for signaling from the ER to the nucleus

1993 • 814 citations

Peptide-binding specificity of the molecular chaperone BiP

1991 • 763 citations

KAR2, a karyogamy gene, is the yeast homolog of the mammalian BiP/GRP78 gene

1989 • 684 citations

Affinity panning of a library of peptides displayed on bacteriophages reveals the binding specificity of BiP

1993 • 650 citations

Heat shock proteins: molecular chaperones of protein biogenesis

1993 • 598 citations

DnaK, DnaJ and GrpE form a cellular chaperone machinery capable of repairing heat-induced protein damage.

1993 • 593 citations

The translation machinery and 70 kd heat shock protein cooperate in protein synthesis

1992 • 512 citations

Molecular evolution of the HSP70 multigene family

1994 • 509 citations

Loss of BiP/GRP78 function blocks translocation of secretory proteins in yeast.

1990 • 435 citations

S. cerevisiae encodes an essential protein homologous in sequence and function to mammalian BiP

1989 • 421 citations

The scavenging of superoxide radical by manganous complexes: In vitro

1982 • 416 citations

A 22 bp cis-acting element is necessary and sufficient for the induction of the yeast KAR2 (BiP) gene by unfolded proteins.

1992 • 375 citations

Sec61p and BiP directly facilitate polypeptide translocation into the ER

1992 • 370 citations

Complex Interactions Among Members of an Essential Subfamily of hsp70 Genes in Saccharomyces Cerevisiae

1987 • 358 citations

Similarity of the three-dimensional structures of actin and the ATPase fragment of a 70-kDa heat shock cognate protein.

1991 • 352 citations

Multiple genes are required for proper insertion of secretory proteins into the endoplasmic reticulum in yeast.

1989 • 339 citations

The Promoter Region of the Yeast KAR2 (BiP) Gene Contains a Regulatory Domain that Responds to the Presence of Unfolded Proteins in the Endoplasmic Reticulum

1993 • 328 citations

A yeast gene important for protein assembly into the endoplasmic reticulum and the nucleus has homology to DnaJ, an Escherichia coli heat shock protein.

1989 • 296 citations

A Sec63p-BiP complex from yeast is required for protein translocation in a reconstituted proteoliposome.

1993 • 290 citations

Intracellular sorting and processing of a yeast vacuolar hydrolase: proteinase A propeptide contains vacuolar targeting information.

1988 • 287 citations

Characterization of YDJ1: a yeast homologue of the bacterial dnaJ protein.

1991 • 284 citations

Sorting of soluble ER proteins in yeast.

1988 • 270 citations

Can Hsp70 proteins act as force-generating motors?

1995 • 270 citations

[8] Putting the HO gene to work: Practical uses for mating-type switching

1991 • 254 citations

BiP and Sec63p are required for both co- and posttranslational protein translocation into the yeast endoplasmic reticulum.

1995 • 251 citations

Mutations in PMR1 suppress oxidative damage in yeast cells lacking superoxide dismutase

1995 • 241 citations

Yeast and mammalian metallothioneins functionally substitute for yeast copper-zinc superoxide dismutase.

1993 • 230 citations

Mitochondrial molecular chaperones: their role in protein translocation

1994 • 203 citations

BiP/Kar2p serves as a molecular chaperone during carboxypeptidase Y folding in yeast.

1995 • 192 citations

A novel Hsp70 of the yeast ER lumen is required for the efficient translocation of a number of protein precursors.

1996 • 176 citations

Control of folding and membrane translocation by binding of the chaperone DnaJ to nascent polypeptides.

1993 • 163 citations

A yeast DnaJ homologue, Scj1p, can function in the endoplasmic reticulum with BiP/Kar2p via a conserved domain that specifies interactions with Hsp70s.

1995 • 151 citations

The yeast EUG1 gene encodes an endoplasmic reticulum protein that is functionally related to protein disulfide isomerase.

1992 • 150 citations

Genetic interactions between KAR2 and SEC63, encoding eukaryotic homologues of DnaK and DnaJ in the endoplasmic reticulum.

1993 • 135 citations

Self-regulation of 70-kilodalton heat shock proteins in Saccharomyces cerevisiae.

1990 • 127 citations

Transcriptional regulation of SSA3, an HSP70 gene from Saccharomyces cerevisiae.

1990 • 125 citations

Interaction between BiP and Sec63p is required for the completion of protein translocation into the ER of Saccharomyces cerevisiae.

1995 • 113 citations

Structure and regulation of the SSA4 HSP70 gene of Saccharomyces cerevisiae.

1990 • 97 citations

Intracellular Mn(II)-associated Superoxide Scavenging Activity Protects Cu, Zn Superoxide Dismutase-deficient Saccharomyces cerevisiae against Dioxygen Stress

1989 • 96 citations

The FKB2 gene of Saccharomyces cerevisiae, encoding the immunosuppressant-binding protein FKBP-13, is regulated in response to accumulation of unfolded proteins in the endoplasmic reticulum.

1993 • 90 citations

The cold sensitivity of a mutant of Saccharomyces cerevisiae lacking a mitochondrial heat shock protein 70 is suppressed by loss of mitochondrial DNA.

1996 • 80 citations

A Heat Shock Transcription Factor with Reduced Activity Suppresses a Yeast HSP70 Mutant

1995 • 62 citations

Biosynthesis of yeast mannoproteins. Synthesis of mannan outer chain and of dolichol derivatives.

1979 • 58 citations

Purification and characterization of BiP/Kar2 protein from Saccharomyces cerevisiae.

1992 • 56 citations

Catalysis of superoxide dismutation by manganese aminopolycarboxylate complexes

1986 • 55 citations

2 Cytosolic hsp70s of Saccharomyces cerevisiae : Roles in Protein Synthesis, Protein Translocation, Proteolysis, and Regulation

1994 • 54 citations

Studies on the acceptor specificity of asparagine‐N‐glycosyl‐transferase from rat liver

1979 • 52 citations

Interorganelle transfer and glycosylation of yeast invertase in vitro.

1986 • 52 citations

Products of reaction of superoxide and hydroxyl radicals with manganese(2+) cation

1978 • 43 citations

Isolation and characterization of extragenic suppressors of mutations in the SSA hsp70 genes of Saccharomyces cerevisiae.

1992 • 38 citations

4 Heat Shock Cognate Proteins and Polypeptide Translocation Across the Endoplasmic Reticulum Membrane

1994 • 23 citations

XI. Yeast sequencing reports. Sequence of a 20·7 kb region of yeast chromosome XI includes theNUP100 gene, an open reading frame (ORF) possibly representing a nucleoside diphosphate kinase gene, tRNAS for His, Val and Trp in addition to seven ORFs with weak or no significant similarity to known proteins

1994 • 10 citations

KAR2

1994 • 1 citations