Eukaryotic proteins expressed in Escherichia coli: An improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

1988 • 6,107 citations

The purification of eukaryotic polypeptides synthesized in Escherichia coli

1986 • 838 citations

Purification of glutathione S-transferases from human liver by glutathione-affinity chromatography

1977 • 481 citations

Immobilization and purification of enzymes with staphylococcal protein A gene fusion vectors.

1985 • 331 citations

Thrombin specificity

1985 • 204 citations

A Polypeptide Fusion Designed for the Purification of Recombinant Proteins

1984 • 146 citations

Production of Leukemia Inhibitory Factor in Escherichia coli by a Novel Procedure and Its Use in Maintaining Embryonic Stem Cells in Culture

1989 • 84 citations

Production of specific antibodies against protein A fusion proteins.

1986 • 77 citations

Purification and Characterization of a 43-kDa Transcription Factor Required for Rat Somatostatin Gene Expression

1989 • 31 citations