Studies on the transformation of intact yeast cells by the LiAc/SS‐DNA/PEG procedure

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

1989 • 8,705 citations

Transformation of intact yeast cells treated with alkali cations

1983 • 7,688 citations

Improved method for high efficiency transformation of intact yeast cells

1992 • 3,258 citations

New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites

1988 • 2,903 citations

[1] Production of single-stranded plasmid DNA

1987 • 2,642 citations

[1] Getting started with yeast

1991 • 2,456 citations

High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier

1989 • 2,140 citations

Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification

1970 • 1,556 citations

Cloning of Large Segments of Exogenous DNA into Yeast by Means of Artificial Chromosome Vectors

1987 • 1,517 citations

High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.

1979 • 1,504 citations

[105] Determination of DNA concentration with diphenylamine

1968 • 1,300 citations

MOLECULAR CHAPERONES

1991 • 1,089 citations

Transformation in yeast: Development of a hybrid cloning vector and isolation of the can1 gene

1979 • 1,080 citations

DMSO-enhanced whole cell yeast transformation

1991 • 534 citations

The fractionation of protein mixtures by linear polymers of high molecular weight

1964 • 379 citations

Saccharomyces cerevisiae mannoproteins form an external cell wall layer that determines wall porosity

1984 • 328 citations

Transformation of yeast spheroplasts without cell fusion

1987 • 266 citations

RAD1, an excision repair gene of Saccharomyces cerevisiae, is also involved in recombination.

1988 • 209 citations

A rapid and efficient procedure for the purification of DNA from agarose gels

1980 • 174 citations

Passage of molecules through yeast cell walls: A brief essay‐review

1991 • 164 citations

Fusion of yeast spheroplasts

1977 • 145 citations

Analysis of the mechanism for reversion of a disrupted gene.

1988 • 114 citations

[53] Isolation of genes by complementation in yeast

1987 • 95 citations

[23] Manipulating yeast genome using plasmid vectors

1990 • 77 citations

Introducing DNA into Yeast by Transformation

1993 • 76 citations

A colony procedure for transformation of Saccharomyces cerevisiae

1988 • 75 citations

Transformation of Protoplasted Yeast Cells Is Directly Associated with Cell Fusion

1984 • 59 citations

Factors Enhancing Genetic Transformation of Intact Yeast Cells Modify Cell Wall Porosity

1986 • 52 citations

Transformation of protoplasted yeast cells is directly associated with cell fusion.

1984 • 51 citations

Variation in frequency of transformation by plasmid YRp7 in Saccharomyces cerevisiae

1981 • 22 citations

Rapid colony transformation ofSaccharomyces cerevisiae

1991 • 14 citations

Intact cell transformation of Saccharomyces cerevisiae by polyethylene glycol.

1985 • 14 citations