Sequences 5' to the polyadenylation signal mediate differential poly(A) site use in hepatitis B viruses.

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease

1979 • 22,482 citations

A new technique for the assay of infectivity of human adenovirus 5 DNA

1973 • 9,740 citations

Rapid and efficient site-specific mutagenesis without phenotypic selection.

1985 • 7,573 citations

Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter

1984 • 6,459 citations

3′ Non-coding region sequences in eukaryotic messenger RNA

1976 • 3,001 citations

THE MOLECULAR BIOLOGY OF THE HEPATITIS B VIRUSES

1987 • 1,046 citations

Two mRNAs can be produced from a single immunoglobulin μ gene by alternative RNA processing pathways

1980 • 880 citations

The sequence 5′-AAUAAA-3′ forms part of the recognition site for polyadenylation of late SV40 mRNAs

1981 • 819 citations

The consensus sequence YGTGTTYY located downstream from the AATAAA signal is required for efficient formation of mRNA 3′ termini

1985 • 652 citations

Activation and suppression of pp60c-src transforming ability by mutation of its primary sites of tyrosine phosphorylation

1987 • 648 citations

Synthesis of secreted and membrane-bound immunoglobulin mu heavy chains is directed by mRNAs that differ at their 3′ ends

1980 • 562 citations

Role of the Conserved AAUAAA Sequence: Four AAUAAA Point Mutants Prevent Messenger RNA 3′ End Formation

1984 • 561 citations

Efficient infection of monkey cells with DNA of simian virus 40.

1981 • 419 citations

Accurate cleavage and polyadenylation of exogenous RNA substrate

1985 • 334 citations

Definition of an efficient synthetic poly(A) site.

1989 • 285 citations

Inhibition of RNA cleavage but not polyadenylation by a point mutation in mRNA 3′ consensus sequence AAUAAA

1983 • 278 citations

Regulation of adenovirus-2 gene expression at the level of transcriptional termination and RNA processing

1981 • 261 citations

Requirement of a downstream sequence for generation of a poly(A) addition site

1984 • 261 citations

Formation of the 3′ end of U1 snRNA requires compatible snRNA promoter elements

1986 • 255 citations

A sequence downstream of AAUAAA is required for rabbit β-globin mRNA 3′-end formation

1984 • 251 citations

Position-dependent sequence elements downstream of AAUAAA are required for efficient rabbit β-globin mRNA 3′ end formation

1987 • 229 citations

Site-specific polyadenylation in a cell-free reaction

1984 • 223 citations

3′ end formation of U1 snRNA precursors is coupled to transcription from snRNA promoters

1986 • 222 citations

Efficiency of Utilization of the Simian Virus 40 Late Polyadenylation Site: Effects of Upstream Sequences

1989 • 205 citations

3′ cleavage and polyadenylation of mRNA precursors in vitro requires a poly(A) polymerase, a cleavage factor, and a snRNP

1988 • 205 citations

Splice commitment dictates neuron-specific alternative RNA processing in calcitonin/CGRP gene expression

1987 • 204 citations

Separation and characterization of a poly(A) polymerase and a cleavage/specificity factor required for pre-mRNA polyadenylation

1988 • 195 citations

Nonmuscle and Muscle Tropomyosin Isoforms Are Expressed from a Single Gene by Alternative RNA Splicing and Polyadenylation

1986 • 190 citations

Sequences capable of restoring poly(A) site function define two distinct downstream elements.

1986 • 175 citations

Analysis of processing and polyadenylation signals of the hepatitis B virus surface antigen gene by using simian virus 40-hepatitis B virus chimeric plasmids.

1983 • 164 citations

The regulated production of mu m and mu s mRNA is dependent on the relative efficiencies of mu s poly(A) site usage and the c mu 4-to-M1 splice.

1989 • 164 citations

Construction of a modular dihydrofolate reductase cDNA gene: analysis of signals utilized for efficient expression.

1982 • 145 citations

A sequence downstream of A-A-U-A-A-A is required for formation of simian virus 40 late mRNA 3' termini in frog oocytes.

1985 • 139 citations

Circles with two tandem LTRs are precursors to integrated retrovirus DNA

1984 • 138 citations

Mapping the major transcripts of ground squirrel hepatitis virus: the presumptive template for reverse transcriptase is terminally redundant

1985 • 122 citations

Requirement for the 3' flanking region of the bovine growth hormone gene for accurate polyadenylylation.

1984 • 109 citations

Sequences upstream of AAUAAA influence poly(A) site selection in a complex transcription unit.

1989 • 108 citations

Identification of a sequence element on the 3' side of AAUAAA which is necessary for simian virus 40 late mRNA 3'-end processing.

1985 • 105 citations

Poly(A) polymerase purified from HeLa cell nuclear extract is required for both cleavage and polyadenylation of pre-mRNA in vitro.

1989 • 104 citations

Definition of essential sequences and functional equivalence of elements downstream of the adenovirus E2A and the early simian virus 40 polyadenylation sites.

1985 • 102 citations

A promoterless retroviral vector indicates that there are sequences in U3 required for 3' RNA processing.

1987 • 100 citations

RNA 3' END FORMATION IN THE CONTROL OF GENE EXPRESSION

1987 • 98 citations

Accurate and specific polyadenylation of mRNA precursors in a soluble whole-cell lysate

1983 • 94 citations

Role of an RNA cleavage/poly(A) addition site in the production of membrane-bound and secreted IgM mRNA.

1985 • 93 citations

Poly(A) site choice rather than splice site choice governs the regulated production of IgM heavy-chain RNAs.

1988 • 87 citations

The cloned genome of ground squirrel hepatitis virus is infectious in the animal.

1984 • 65 citations

Mutations downstream of the polyadenylation site of a Xenopus β-globin mRNA affect the position but not the efficiency of 3′ processing

1986 • 63 citations

Regulation of poly(A) site selection in adenovirus

1989 • 61 citations

Identification of sequences in the herpes simplex virus thymidine kinase gene required for efficient processing and polyadenylation.

1985 • 60 citations

Identification of a U5-specific sequence required for efficient polyadenylation within the human immunodeficiency virus long terminal repeat

1989 • 52 citations

Replication of duck hepatitis B virus in two differentiated human hepatoma cell lines after transfection with cloned viral DNA

1988 • 44 citations

Kinetics and efficiency of polyadenylation of late polyomavirus nuclear RNA: generation of oligomeric polyadenylated RNAs and their processing into mRNA.

1984 • 42 citations

Fine-structure analysis of the processing and polyadenylation region of the herpes simplex virus type 1 thymidine kinase gene by using linker scanning, internal deletion, and insertion mutations.

1986 • 41 citations

Role of poly(A) polymerase in the cleavage and polyadenylation of mRNA precursor.

1989 • 36 citations

ATTAAA As Well As Downstream Sequences Are Required for RNA 3′-End Formation in the E3 Complex Transcription Unit of Adenovirus

1985 • 35 citations

Requirement of A-A-U-A-A-A and adjacent downstream sequences for SV40 early potyadenylation

1986 • 30 citations