3′ cleavage and polyadenylation of mRNA precursors in vitro requires a poly(A) polymerase, a cleavage factor, and a snRNP

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

1976 • 200,127 citations

Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei

1983 • 11,902 citations

Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter

1984 • 6,459 citations

3′ Non-coding region sequences in eukaryotic messenger RNA

1976 • 3,001 citations

Transcription termination and 3′ processing: the end is in site!

1985 • 1,482 citations

The sequence 5′-AAUAAA-3′ forms part of the recognition site for polyadenylation of late SV40 mRNAs

1981 • 819 citations

The consensus sequence YGTGTTYY located downstream from the AATAAA signal is required for efficient formation of mRNA 3′ termini

1985 • 652 citations

Role of the Conserved AAUAAA Sequence: Four AAUAAA Point Mutants Prevent Messenger RNA 3′ End Formation

1984 • 561 citations

A factor, U2AF, is required for U2 snRNP binding and splicing complex assembly

1988 • 535 citations

Stepwise assembly of a pre-mRNA splicing complex requires U-snRNPs and specific intron sequences

1985 • 524 citations

COMPLEX TRANSCRIPTIONAL UNITS: DIVERSITY IN GENE EXPRESSION BY ALTERNATIVE RNA PROCESSING

1986 • 486 citations

Multiple factors including the small nuclear ribonucleoproteins U1 and U2 are necessary for Pre-mRNA splicing in vitro

1985 • 442 citations

α-Thalassaemia caused by a polyadenylation signal mutation

1983 • 407 citations

The 5′ terminus of the RNA moiety of U1 small nuclear ribonucleoprotein particles is required for the splicing of messenger RNA precursors

1984 • 404 citations

Accurate cleavage and polyadenylation of exogenous RNA substrate

1985 • 334 citations

The 3′ Splice Site of Pre-Messenger RNA Is Recognized by a Small Nuclear Ribonucleoprotein

1985 • 310 citations

Inhibition of RNA cleavage but not polyadenylation by a point mutation in mRNA 3′ consensus sequence AAUAAA

1983 • 278 citations

A protein that specifically recognizes the 3′ splice site of mammalian pre-mRNA introns is associated with a small nuclear ribonucleoprotein

1986 • 274 citations

Requirement of a downstream sequence for generation of a poly(A) addition site

1984 • 261 citations

Splicing of in vitro synthesized messenger RNA precursors in HeLa cell extracts

1983 • 255 citations

A sequence downstream of AAUAAA is required for rabbit β-globin mRNA 3′-end formation

1984 • 251 citations

Isolation of small nuclear ribonucleoproteins containing U1, U2, U4, U5, and U6 RNAs.

1983 • 245 citations

Site-specific polyadenylation in a cell-free reaction

1984 • 223 citations

Identification of the Human U7 snRNP as One of Several Factors Involved in the 3′ End Maturation of Histone Premessenger RNA's

1987 • 221 citations

Small Nuclear RNAs: RNA Sequences, Structure, and Modifications

1988 • 202 citations

Separation and characterization of a poly(A) polymerase and a cleavage/specificity factor required for pre-mRNA polyadenylation

1988 • 195 citations

U1, U2, and U4/U6 small nuclear ribonucleoproteins are required for in vitro splicing but not polyadenylation

1986 • 192 citations

A 64 kd nuclear protein binds to RNA segments that include the AAUAAA polyadenylation motif

1988 • 187 citations

Poly(A) site cleavage in a HeLa nuclear extract is dependent on downstream sequences

1985 • 152 citations

Simple, efficientin vitrosynthesis of capped RNA useful for direct expression of cloned eukaryoti genes

1982 • 152 citations

The AAUAAA sequence is required both for cleavage and for polyadenylation of simian virus 40 pre-mRNA in vitro.

1986 • 147 citations

A small nuclear ribonucleoprotein associates with the AAUAAA polyadenylation signal in vitro

1986 • 139 citations

Enzymes of Nucleic Acid Synthesis and Modification

2018 • 116 citations

Specific contacts between mammalian U7 snRNA and histone precursor RNA are indispensable for the in vitro 3′ RNA processing reaction.

1988 • 106 citations

Multiple factors are required for specific RNA cleavage at a poly(A) addition site.

1988 • 106 citations

Identification of a sequence element on the 3' side of AAUAAA which is necessary for simian virus 40 late mRNA 3'-end processing.

1985 • 105 citations

The conserved CAAGAAAGA spacer sequence is an essential element for the formation of 3′ termini of the sea urchin H3 histone mRNA by RNA processing.

1985 • 99 citations

RNA sequence containing hexanucleotide AAUAAA directs efficient mRNA polyadenylation in vitro.

1985 • 95 citations

Analysis of RNA cleavage at the adenovirus-2 L3 polyadenylation site.

1986 • 94 citations

Cleavage and polyadenylation of messenger RNA precursors in vitro occurs within large and specific 3′ processing complexes.

1987 • 88 citations

Electrophoretic separation of polyadenylation-specific complexes.

1987 • 84 citations

Structural and functional characterization of mouse U7 small nuclear RNA active in 3' processing of histone pre-mRNA.

1988 • 78 citations

Analysis of mRNA 3′ end formation by modification interference: the only modifications which prevent processing lie in AAUAAA and the poly(A) site.

1987 • 77 citations

Purification of a protein required for the splicing of pre-mRNA and its separation from the lariat debranching enzyme.

1985 • 72 citations

Isolation and partial characterization of the poly(A) polymerases from HeLa cells infected with vaccinia virus.

1977 • 66 citations

Multiple factors are required for poly(A) addition to a mRNA 3' end.

1988 • 65 citations

Nuclear Poly(A) Polymerase from Rat Liver and a Hepatoma

1976 • 65 citations

Products of in vitro cleavage and polyadenylation of simian virus 40 late pre-mRNAs.

1987 • 64 citations

A functionally redundant downstream sequence in SV40 late pre-mRNA is required for mRNA 3'-end formation and for assembly of a precleavage complex in vitro.

1988 • 64 citations

RNA-protein organization of U1, U5 and U4-U6 small nuclear ribonucleoproteins in HeLa cells

1986 • 62 citations

Identification of a complex associated with processing and polyadenylation in vitro of herpes simplex virus type 1 thymidine kinase precursor RNA.

1987 • 59 citations

Specific pre-cleavage and post-cleavage complexes involved in the formation of SV40 late mRNA 3′ termini in vitro.

1987 • 57 citations

Separation of multiple components of HeLa cell nuclear extracts required for pre-messenger RNA splicing.

1987 • 56 citations

Requirements for accurate and efficient mRNA 3' end cleavage and polyadenylation of a simian virus 40 early pre-RNA in vitro.

1987 • 56 citations

8 Poly(A) Adding Enzymes

1982 • 48 citations

Sedimentation analysis of polyadenylation-specific complexes.

1988 • 42 citations

In vitro cleavage of the simian virus 40 early polyadenylation site adjacent to a required downstream TG sequence.

1986 • 36 citations

Assembly of a polyadenylation-specific 25S ribonucleoprotein complex in vitro.

1988 • 35 citations

Fractionation of HeLa cell nuclear extracts reveals minor small nuclear ribonucleoprotein particles.

1987 • 32 citations

Mutations in poly(A) site downstream elements affect in vitro cleavage activity.

1988 • 28 citations

Poly(A) Polymerase from Eukaryotes

2018 • 12 citations