Prospective Characterization of Full-Length Hepatitis C Virus NS5A Quasispecies during Induction and Combination Antiviral Therapy

Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

1987 • 64,270 citations

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

1987 • 45,673 citations

CONFIDENCE LIMITS ON PHYLOGENIES: AN APPROACH USING THE BOOTSTRAP

1985 • 39,646 citations

PHYLIP-Phylogeny inference package (Version 3.2)

1989 • 17,674 citations

Interferon Alfa-2b Alone or in Combination with Ribavirin as Initial Treatment for Chronic Hepatitis C

1998 • 3,550 citations

Genetic organization and diversity of the hepatitis C virus.

1991 • 1,717 citations

Interferon Alfa-2b Alone or in Combination with Ribavirin for the Treatment of Relapse of Chronic Hepatitis C

1998 • 1,318 citations

Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis.

1990 • 1,208 citations

Mutations in the Nonstructural Protein 5a Gene and Response to Interferon in Patients with Chronic Hepatitis C Virus 1b Infection

1996 • 1,016 citations

Hepatitis C virus (HCV) circulates as a population of different but closely related genomes: quasispecies nature of HCV genome distribution

1992 • 938 citations

Evidence That Hepatitis C Virus Resistance to Interferon Is Mediated through Repression of the PKR Protein Kinase by the Nonstructural 5A Protein

1997 • 824 citations

Analysis of a Successful Immune Response against Hepatitis C Virus

1999 • 823 citations

Meta-analysis of interferon randomized trials in the treatment of viral hepatitis C: Effects of dose and duration

1996 • 675 citations

Comparison of full-length sequences of interferon-sensitive and resistant hepatitis C virus 1b. Sensitivity to interferon is conferred by amino acid substitutions in the NS5A region.

1995 • 638 citations

Control of PKR Protein Kinase by Hepatitis C Virus Nonstructural 5A Protein: Molecular Mechanisms of Kinase Regulation

1998 • 603 citations

RNA Virus Populations as Quasispecies

1992 • 523 citations

Hepatitis C Virus Type 1b (II) Infection in France and Italy

1995 • 490 citations

Pretreatment serum hepatitis C virus RNA levels and hepatitis C virus genotype are the main and independent prognostic factors of sustained response to interferon alfa therapy in chronic hepatitis C

1995 • 474 citations

Identification of a highly conserved sequence element at the 3' terminus of hepatitis C virus genome RNA

1996 • 450 citations

Survey of major genotypes and subtypes of hepatitis C virus using RFLP of sequences amplified from the 5' non-coding region

1995 • 419 citations

Genomic structure of the human prototype strain H of hepatitis C virus: comparison with American and Japanese isolates.

1991 • 281 citations

Phosphorylation of hepatitis C virus-encoded nonstructural protein NS5A

1995 • 281 citations

NS5A, a nonstructural protein of hepatitis C virus, binds growth factor receptor-bound protein 2 adaptor protein in a Src homology 3 domain/ligand-dependent manner and perturbs mitogenic signaling

1999 • 229 citations

New methods for estimating the numbers of synonymous and nonsynonymous substitutions

1995 • 220 citations

Production of Two Phosphoproteins from the NS5A Region of the Hepatitis C Viral Genome

1994 • 217 citations

Pretreatment Virus Load and Multiple Amino Acid Substitutions in the Interferon Sensitivity-Determining Region Predict the Outcome of Interferon Treatment in Patients With Chronic Genotype 1B Hepatitis C Virus Infection

1997 • 199 citations

Interferon Resistance of Hepatitis C Virus Genotype 1b: Relationship to Nonstructural 5A Gene Quasispecies Mutations

1998 • 199 citations

Mutations in the nonstructural 5A gene of European hepatitis C virus isolates and response to interferon alfa

1997 • 194 citations

Acute Hepatitis C Virus Structural Gene Sequences as Predictors of Persistent Viremia: Hypervariable Region 1 as a Decoy

1999 • 193 citations

Phosphorylation of the hepatitis C virus NS5A protein in vitro and in vivo: properties of the NS5A-associated kinase

1997 • 177 citations

Analysis of genotypes and amino acid residues 2209 to 2248 of the NS5A region of hepatitis C virus in relation to the response to interferon-? therapy

1997 • 166 citations

Hepatitis C virus nonstructural region 5A protein is a potent transcriptional activator

1997 • 150 citations

Contribution of Taq polymerase-induced errors to the estimation of RNA virus diversity.

1998 • 147 citations

Sequence analysis of the NS5A protein of European hepatitis C virus 1b isolates and relation to interferon sensitivity.

1998 • 145 citations

Characterization of the effects of hepatitis C virus nonstructural 5A protein expression in human cell lines and on interferon-sensitive virus replication

1999 • 137 citations

Mutations in the nonstructural 5A region of hepatitis C virus and response of chronic hepatitis C to interferon alfa

1997 • 136 citations

Evolution of Hepatitis C Virus Quasispecies in Hypervariable Region 1 and the Putative Interferon Sensitivity-Determining Region during Interferon Therapy and Natural Infection

1998 • 135 citations

Cell Mediated Immune Response to the Hepatitis C Virus

2000 • 126 citations

Specificity of the hepatitis C virus NS3 serine protease: effects of substitutions at the 3/4A, 4A/4B, 4B/5A, and 5A/5B cleavage sites on polyprotein processing

1994 • 124 citations

Mutations of hepatitis C virus 1b NS5A 2209–2248 amino acid sequence do not predict the response to recombinant interferon-alfa therapy in French patients

1997 • 124 citations

Mutations in the NS5A gene of hepatitis C virus in North American patients infected with HCV genotype 1a or 1b

1997 • 119 citations

The Prognostic Relevance of the Nonstructural 5A Gene Interferon Sensitivity Determining Region Is Different in Infections with Genotype 1b and 3a Isolates of Hepatitis C Virus

1998 • 108 citations

Characteristics of Nucleotide Substitution in the Hepatitis C Virus Genome: Constraints on Sequence Change in Coding Regions at Both Ends of the Genome

1997 • 107 citations

Genetic complexity of the hypervariable region 1 (HVR1) of hepatitis C virus (HCV): Influence on the characteristics of the infection and responses to interferon alfa therapy in patients with chronic hepatitis C

1998 • 101 citations

Characterization of the nuclear localization signal and subcellular distribution of hepatitis C virus nonstructural protein NS5A

1996 • 99 citations

The entire nucleotide sequence and classification of a hepatitis C virus isolate of a novel genotype from an Indonesian patient with chronic liver disease

1994 • 96 citations

Hepatitis C virus NS5A protein inhibits interferon antiviral activity, but the effects do not correlate with clinical response

1999 • 82 citations

The Amino Terminal Deletion Mutants of Hepatitis C Virus Nonstructural Protein NS5A Function as Transcriptional Activators in Yeast

1997 • 77 citations

Mutations in the Protein Kinase–Binding Domain of the NS5A Protein in Patients Infected with Hepatitis C Virus Type 1a Are Associated with Treatment Response

2000 • 77 citations

Identification of the Major Phosphorylation Site of the Hepatitis C Virus H Strain NS5A Protein as Serine 2321

1999 • 74 citations

Mutations in the NS5A region do not predict interferon-responsiveness in American patients infected with genotype 1b hepatitis C virus

1999 • 71 citations

Hepatitis C virus NS5A protein is phosphorylated in vitro by a stably bound protein kinase from HeLa cells and by cAMP-dependent protein kinase A-α catalytic subunit

1997 • 69 citations

Mutations in the interferon-sensitivity determining region of hepatitis C virus and transcriptional activity of the nonstructural region 5A protein

1998 • 62 citations

Effect of Retreatment with Interferon Alone or Interferon plus Ribavirin on Hepatitis C Virus Quasispecies Diversification in Nonresponder Patients with Chronic Hepatitis C

1999 • 59 citations

Evidence for sequence selection within the non‐structural 5A gene of hepatitis C virus type 1b during unsuccessful treatment with interferon‐α

1999 • 28 citations

Pattern of HCV antibodies with special reference to NS5A reactivity in HCV-infected patients: relation to viral genotype, cryoglobulinemia and response to interferon

1998 • 23 citations