Covalent Modification of the Transactivator Protein IE2-p86 of Human Cytomegalovirus by Conjugation to the Ubiquitin-Homologous Proteins SUMO-1 and hSMT3b

A novel genetic system to detect protein–protein interactions

1989 • 6,022 citations

Improved method for high efficiency transformation of intact yeast cells

1992 • 3,258 citations

A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformaion of Escherichia coli

1987 • 2,493 citations

The CBP co-activator is a histone acetyltransferase

1996 • 1,773 citations

The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit.

1993 • 1,459 citations

A Small Ubiquitin-Related Polypeptide Involved in Targeting RanGAP1 to Nuclear Pore Complex Protein RanBP2

1997 • 1,221 citations

A novel ubiquitin-like modification modulates the partitioning of the Ran-GTPase-activating protein RanGAP1 between the cytosol and the nuclear pore complex.

1996 • 1,161 citations

A very strong enhancer is located upstream of an immediate early gene of human cytomegalovirus

1985 • 1,155 citations

[10] Yeast promoters and lacZ fusions designed to study expression of cloned genes in yeast

1983 • 1,095 citations

SUMO-1 Modification of IκBα Inhibits NF-κB Activation

1998 • 1,002 citations

Potential Role of Human Cytomegalovirus and p53 Interaction in Coronary Restenosis

1994 • 750 citations

Conjugation with the ubiquitin-related modifier SUMO-1 regulates the partitioning of PML within the nucleus

1998 • 676 citations

SUMO-1 modification activates the transcriptional response of p53

1999 • 627 citations

Regulation of the Yeast HO Gene

1985 • 526 citations

Activation of p53 by conjugation to the ubiquitin-like protein SUMO-1

1999 • 507 citations

Ubc9p Is the Conjugating Enzyme for the Ubiquitin-like Protein Smt3p

1997 • 488 citations

Identification of the Enzyme Required for Activation of the Small Ubiquitin-like Protein SUMO-1

1999 • 353 citations

PIC 1, a novel ubiquitin-like protein which interacts with the PML component of a multiprotein complex that is disrupted in acute promyelocytic leukaemia.

1996 • 347 citations

Evidence for Covalent Modification of the Nuclear Dot–associated Proteins PML and Sp100 by PIC1/SUMO-1

1997 • 329 citations

Temporal Patterns of Human Cytomegalovirus Transcription: Mapping the Viral RNAs Synthesized at Immediate Early, Early, and Late Times After Infection

1982 • 311 citations

Identification of Three Major Sentrinization Sites in PML

1998 • 305 citations

Viral Immediate-Early Proteins Abrogate the Modification by SUMO-1 of PML and Sp100 Proteins, Correlating with Nuclear Body Disruption

1999 • 303 citations

Molecular Characterization of the SUMO-1 Modification of RanGAP1 and Its Role in Nuclear Envelope Association

1998 • 290 citations

Organization and expression of the immediate early genes of human cytomegalovirus

1983 • 288 citations

The Nuclear Dot Protein Sp100, Characterization of Domains Necessary for Dimerization, Subcellular Localization, and Modification by Small Ubiquitin-like Modifiers

1999 • 258 citations

The Saccharomyces cerevisiae DNA repair gene RAD23 encodes a nuclear protein containing a ubiquitin-like domain required for biological function.

1993 • 246 citations

trans-activation and autoregulation of gene expression by the immediate-early region 2 gene products of human cytomegalovirus

1988 • 237 citations

Identification and characterization of the human cytomegalovirus immediate-early region 2 gene that stimulates gene expression from an inducible promoter

1987 • 223 citations

Transactivation of a human cytomegalovirus early promoter by gene products from the immediate-early gene IE2 and augmentation by IE1: mutational analysis of the viral proteins

1990 • 216 citations

Regulated expression of early and late RNAs and proteins from the human cytomegalovirus immediate-early gene region

1989 • 216 citations

The Nuclear Domain 10 (ND10) Is Disrupted by the Human Cytomegalovirus Gene Product IE1

1996 • 213 citations

The ubiquitin-like proteins SMT3 and SUMO-1 are conjugated by the UBC9 E2 enzyme

1998 • 210 citations

Transcription in human fibroblasts permissively infected by human cytomegalovirus strain AD169

1983 • 199 citations

Cloning of the complete human cytomegalovirus genome in cosmids

1982 • 198 citations

Promoter-specific trans activation and repression by human cytomegalovirus immediate-early proteins involves common and unique protein domains

1990 • 197 citations

The human cytomegalovirus 80-kilodalton but not the 72-kilodalton immediate-early protein transactivates heterologous promoters in a TATA box-dependent mechanism and interacts directly with TFIID

1992 • 195 citations

Disruption of PML Subnuclear Domains by the Acidic IE1 Protein of Human Cytomegalovirus Is Mediated through Interaction with PML and May Modulate a RING Finger-Dependent Cryptic Transactivator Function of PML

1998 • 176 citations

Preferential Modification of Nuclear Proteins by a Novel Ubiquitin-like Molecule

1997 • 173 citations

Human cytomegalovirus DNA: Restriction enzyme cleavage maps and map locations for immediate-early, early, and late RNAs

1981 • 171 citations

A rapid microneutralization assay for the measurement of neutralizing antibody reactive with human cytomegalovirus

1989 • 168 citations

Functional interaction between the HCMV IE2 transactivator and the retinoblastoma protein.

1994 • 164 citations

Temporal regulation of human cytomegalovirus transcription at immediate early and early times after infection

1981 • 159 citations

The functionally active IE2 immediate-early regulatory protein of human cytomegalovirus is an 80-kilodalton polypeptide that contains two distinct activator domains and a duplicated nuclear localization signal

1991 • 146 citations

The human cytomegalovirus 86K immediate early (IE) 2 protein requires the basic region of the TATA-box binding protein (TBP) for binding, and interacts with TBP and transcription factor TFIIB via regions of IE2 required for transcriptional regulation

1993 • 138 citations

Disruption of PML-associated nuclear bodies mediated by the human cytomegalovirus major immediate early gene product.

1998 • 132 citations

Two Nuclear Dot‐Associated Proteins, PML and SplOO, are Often Co‐Autoimmunogenic in Patients with Primary Biliary Cirrhosis

1995 • 121 citations

Transactivation of the cytomegalovirus ICP36 gene promoter requires the alpha gene product TRS1 in addition to IE1 and IE2

1992 • 120 citations

The 72K IE1 and 80K IE2 proteins of human cytomegalovirus independently trans-activate the c-fos, c-myc and hsp70 promoters via basal promoter elements

1992 • 117 citations

Functional interaction between the human cytomegalovirus 86-kilodalton IE2 protein and the cellular transcription factor CREB

1995 • 114 citations

Transactivation by the human cytomegalovirus IE2 86-kilodalton protein requires a domain that binds to both the TATA box-binding protein and the retinoblastoma protein

1994 • 111 citations

The 86-kilodalton IE-2 protein of human cytomegalovirus is a sequence-specific DNA-binding protein that interacts directly with the negative autoregulatory response element located near the cap site of the IE-1/2 enhancer-promoter

1993 • 110 citations

An in vitro system for human cytomegalovirus immediate early 2 protein (IE2)-mediated site-dependent repression of transcription and direct binding of IE2 to the major immediate early promoter.

1993 • 109 citations

The IE2 regulatory protein of human cytomegalovirus induces expression of the human transforming growth factor beta1 gene through an Egr-1 binding site

1996 • 105 citations

Human cytomegalovirus immediate-early two protein region involved in negative regulation of the major immediate-early promoter

1990 • 100 citations

Identification of binding sites for the 86-kilodalton IE2 protein of human cytomegalovirus within an IE2-responsive viral early promoter

1994 • 93 citations

Molecular Cloning of the cDNA and Chromosome Localization of the Gene for Human Ubiquitin-conjugating Enzyme 9

1996 • 92 citations

In vivo and in vitro analysis of transcriptional activation mediated by the human cytomegalovirus major immediate-early proteins.

1993 • 92 citations

Identification of the structural and functional human homolog of the yeast ubiquitin conjugating enzyme UBC9

1996 • 88 citations

Expression of the human cytomegalovirus UL36–38 immediate early region during permissive infection

1991 • 88 citations

Analysis of Proteins Encoded by IE Regions 1 and 2 of Human Cytomegalovirus Using Monoclonal Antibodies Generated against Recombinant Antigens

1993 • 87 citations

Human cytomegalovirus immediate-early gene 2 protein interacts with itself and with several novel cellular proteins

1993 • 84 citations

Identification and mapping of dimerization and DNA-binding domains in the C terminus of the IE2 regulatory protein of human cytomegalovirus

1993 • 82 citations

The UL84 protein of human cytomegalovirus acts as a transdominant inhibitor of immediate-early-mediated transactivation that is able to prevent viral replication

1997 • 78 citations

The human cytomegalovirus IE2 86-kilodalton protein interacts with an early gene promoter via site-specific DNA binding and protein-protein associations

1995 • 71 citations

mUBC9, a Novel Adenovirus E1A-interacting Protein That Complements a Yeast Cell Cycle Defect

1996 • 69 citations

The Saccharomyces cerevisiae DNA Repair Gene RAD23 Encodes a Nuclear Protein Containing a Ubiquitin-Like Domain Required for Biological Function

1993 • 66 citations

In Vivo and in Vitro Analysis of Transcriptional Activation Mediated by the Human Cytomegalovirus Major Immediate-Early Proteins

1993 • 64 citations

Monoclonal Antibody E-13 (M-810) to Human Cytomegalovirus Recognizes an Epitope Encoded by Exon 2 of the Major Immediate Early Gene

1992 • 62 citations

Cloning and Expression of Human Homolog HSMT3 to Yeast SMT3 Suppressor of MIF2 Mutations in a Centromere Protein Gene

1996 • 53 citations

Two versatile eukaryotic expression vectors permitting epitope tagging, radiolabelling and nuclear localisation of expressed proteins

1996 • 43 citations

Cytomegalovirus IE2 Protein Stimulates Interleukin 1β Gene Transcription via Tethering to Spi-1/PU.1

1999 • 42 citations

Modulation of ETS-1 transcriptional activity by huUBC9, a ubiquitin-conjugating enzyme

1997 • 39 citations

Mutations of the Human Cytomegalovirus Immediate-Early 2 Protein Defines Regions and Amino Acid Motifs Important in Transactivation of Transcription from the HIV-1 LTR Promoter

1993 • 37 citations

Inhibition of the association of RNA polymerase II with the preinitiation complex by a viral transcriptional repressor.

1996 • 31 citations

Breast Milk Transmission of Cytomegalovirus (CMV) Infection

1991 • 14 citations