DNA Polymerase Fidelity: From Genetics Toward a Biochemical Understanding

Enzyme structure and mechanism

1977 • 2,935 citations

Enzyme structure and mechanism

1978 • 2,897 citations

Kinetic Proofreading: A New Mechanism for Reducing Errors in Biosynthetic Processes Requiring High Specificity

1974 • 1,532 citations

Frameshift Mutations and the Genetic Code

1966 • 1,400 citations

Molecular basis of base substitution hotspots in Escherichia coli

1978 • 1,185 citations

Enthalpy–entropy compensation phenomena in water solutions of proteins and small molecules: A ubiquitous properly of water

1970 • 1,173 citations

Kinetic amplification of enzyme discrimination

1975 • 742 citations

FIDELITY MECHANISMS IN DNA REPLICATION

1991 • 709 citations

Enzymatic Synthesis of Deoxyribonucleic Acid

1972 • 594 citations

ON THE TOPOGRAPHY OF THE GENETIC FINE STRUCTURE

1961 • 576 citations

CONFORMATIONAL COUPLING IN DNA POLYMERASE FIDELITY

1993 • 558 citations

Structure of DNA Polymerase I Klenow Fragment Bound to Duplex DNA

1993 • 473 citations

Biochemical Basis of DNA Replication Fidelity

1993 • 441 citations

Crystal Structure of a pol α Family Replication DNA Polymerase from Bacteriophage RB69

1997 • 431 citations

Base-base mismatches. Thermodynamics of double helix formation for dCA₃XA₃G + dCT₃YT₃G (X, Y = A,C,G,D

1985 • 426 citations

An induced-fit kinetic mechanism for DNA replication fidelity: direct measurement by single-turnover kinetics

1991 • 364 citations

Kinetic mechanism of DNA polymerase I (Klenow)

1987 • 352 citations

Genetic and Crystallographic Studies of the 3′,5′-Exonucleolytic Site of DNA Polymerase I

1988 • 344 citations

Studies on the Biochemical Basis of Spontaneous Mutation

1972 • 340 citations

Comparison between DNA melting thermodynamics and DNA polymerase fidelity.

1988 • 319 citations

The mutational specificity of DNA polymerase-beta during in vitro DNA synthesis. Production of frameshift, base substitution, and deletion mutations.

1985 • 317 citations

A thymidine triphosphate shape analog lacking Watson–Crick pairing ability is replicated with high sequence selectivity

1997 • 309 citations

Trinucleotide repeats associated with human disease

1997 • 288 citations

Kinetic mechanism whereby DNA polymerase I (Klenow) replicates DNA with high fidelity

1988 • 278 citations

Mutagenesis by transient misalignment.

1988 • 265 citations

Nearest Neighbor Influences on DNA Polymerase Insertion Fidelity

1989 • 247 citations

Mutagenic DNA polymerase

1965 • 227 citations

Hydrogen bonding revisited: Geometric selection as a principal determinant of DNA replication fidelity

1997 • 214 citations

Kinetic characterization of the polymerase and exonuclease activities of the gene 43 protein of bacteriophage T4

1992 • 207 citations

Spontaneous Mutation: Genetic Control of Mutation Rates in Bacteriophage T4

1969 • 206 citations

Mechanisms of mutagenesis in the Escherichia coli mutator mutD5: role of DNA mismatch repair.

1988 • 203 citations

[10] Extracting thermodynamic data from equilibrium melting curves for oligonucleotide order-disorder transitions

1995 • 193 citations

Frameshift mutagenesis by eucaryotic DNA polymerases in vitro.

1986 • 177 citations

Studies on the biochemical basis of spontaneous mutation

1974 • 170 citations

Error induction and correction by mutant and wild type T4 DNA polymerases. Kinetic error discrimination mechanisms.

1979 • 167 citations

Fidelity of replication of phage phi X174 DNA by DNA polymerase III holoenzyme: spontaneous mutation by misincorporation.

1979 • 165 citations

Abasic Translesion Synthesis by DNA Polymerase β Violates the “A-rule”

1997 • 163 citations

Spectroscopic and Calorimetric Characterizations of DNA Duplexes Containing 2-Aminopurine

1996 • 158 citations

The structural gene for deoxyribonucleic acid polymerase in bacteriophages T4 and T5.

1965 • 157 citations

Comparison of nucleotide interactions in water, proteins, and vacuum: model for DNA polymerase fidelity.

1986 • 148 citations

The nucleotide analog 2-aminopurine as a spectroscopic probe of nucleotide incorporation by the Klenow fragment of Escherichia coli polymerase I and bacteriophage T4 DNA polymerase

1995 • 128 citations

Structure-Energy Analysis of the Role of Metal Ions in Phosphodiester Bond Hydrolysis by DNA Polymerase I

1995 • 128 citations

Fidelity of Escherichia coli DNA Polymerase III Holoenzyme

1997 • 120 citations

On the Role of DNA Polymerase in Base Selection

1966 • 118 citations

Antimutagenic DNA Polymerases of Bacteriophage T4

1968 • 114 citations

ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID, X. INFLUENCE OF BROMOURACIL SUBSTITUTIONS ON REPLICATION

1962 • 110 citations

Enthalpy-Entropy Compensation in DNA Melting Thermodynamics

1995 • 108 citations

Hydrolysis of Template and Newly Synthesized Deoxyribonucleic Acid by the 3′ to 5′ Exonuclease Activity of the T4 Deoxyribonucleic Acid Polymerase

1972 • 105 citations

Pre-Steady-State Kinetic Analysis of Sequence-Dependent Nucleotide Excision by the 3'-Exonuclease Activity of Bacteriophage T4 DNA Polymerase

1994 • 99 citations

Influence of neighboring bases on DNA polymerase insertion and proofreading fidelity.

1985 • 98 citations

2-Aminopurine

1980 • 93 citations

The energy relay: a proofreading scheme based on dynamic cooperativity and lacking all characteristic symptoms of kinetic proofreading in DNA replication and protein synthesis.

1980 • 91 citations

Enzymatic determinants of DNA polymerase accuracy. Theory of coliphage T4 polymerase mechanisms

1978 • 90 citations

Influence of 5'-nearest neighbors on the insertion kinetics of the fluorescent nucleotide analog 2-aminopurine by Klenow fragment

1993 • 86 citations

On the Role of Deoxyribonucleic Acid Polymerase in Determining Mutation Rates

1973 • 85 citations

Control of mutation frequency by bacteriophage T4 DNA polymerase. I. The CB120 antimutator DNA polymerase is defective in strand displacement.

1976 • 84 citations

Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants

1988 • 84 citations

Dynamics of Bacteriophage T4 DNA Polymerase Function: Identification of Amino Acid Residues that Affect Switching between Polymerase and 3′ → 5′ Exonuclease Activities

1995 • 82 citations

The minimal kinetic mechanism for misincorporation by DNA polymerase I (Klenow fragment)

1992 • 76 citations

Studies on the biochemical basis of spontaneous mutation

1977 • 76 citations

Motif A of bacteriophage T4 DNA polymerase: role in primer extension and DNA replication fidelity. Isolation of new antimutator and mutator DNA polymerases.

1994 • 74 citations

Gel Kinetic Analysis of DNA Polymerase Fidelity in the Presence of Proofreading Using Bacteriophage T4 DNA Polymerase

1995 • 73 citations

The fidelity of base selection by the polymerase subunit of DNA polymerase III holoenzyme

1988 • 64 citations

The Influence of Neighboring Base Pairs upon Base-Pair Substitution Mutation Rates

1971 • 63 citations

On the molecular basis of transition mutations: frequencies of forming 2-aminopurine.cytosine and adenine.cytosine base mispairs in vitro.

1981 • 63 citations

General Antimutators are Improbable

1993 • 62 citations

ON THE SPECIFICITY OF DNA POLYMERASE

1967 • 58 citations

Studies on the biochemical basis of spontaneous mutation

1974 • 53 citations

Kinetic measurement of 2-aminopurine X cytosine and 2-aminopurine X thymine base pairs as a test of DNA polymerase fidelity mechanisms.

1982 • 53 citations

Mutation rate : Some biological and biochemical considerations

1982 • 52 citations

Studies on the biochemical basis of mutation

1977 • 50 citations

Studies on the Biology

1971 • 50 citations

A single mutation in bacteriophage T4 DNA polymerase (A737V, tsL141) decreases its processivity as a polymerase and increases its processivity as a 3‘–>5‘ exonuclease.

1994 • 46 citations

2-Aminopurine-induced mutagenesis in T4 bacteriophage: a model relating mutation frequency to 2-aminopurine incorporation in DNA.

1977 • 45 citations

The half-of-the-sites reactivity of horse liver alcohol dehydrogenase in the presence of alcohol substrates

1974 • 44 citations

Mutagen specificity and position effects on mutation in T4rII nonsense sites

1976 • 43 citations

Influence of local nucleotide sequence on substitution of 2-aminopurine for adenine during deoxyribonucleic acid synthesis in vitro

1983 • 39 citations

A MAJOR ROLE FOR BACTERIOPHAGE T4 DNA POLYMERASE IN FRAMESHIFT MUTAGENESIS

1983 • 35 citations

Locations of amino acid substitutions in bacteriophage T4 tsL56 DNA polymerase predict an N-terminal exonuclease domain

1989 • 32 citations

Learning about DNA polymerase function by studying antimutator DNA polymerase

1995 • 22 citations

Roots: Mutation Rates

1992 • 10 citations

Ribonucleoside and deoxyribonucleoside triphosphate pools during 2-aminopurine mutagenesis in T4 mutator-, wild type-, and antimutator-infected Escherichia coli.

1985 • 8 citations