Direct in Vivo Screening of Intrabody Libraries Constructed on a Highly Stable Single-chain Framework

Methods in yeast genetics

1979 • 7,824 citations

MOLMOL: A program for display and analysis of macromolecular structures

1996 • 7,169 citations

A novel genetic system to detect protein–protein interactions

1989 • 6,022 citations

Methods in Yeast Genetics

1988 • 4,905 citations

The Leucine Zipper: A Hypothetical Structure Common to a New Class of DNA Binding Proteins

1988 • 3,479 citations

Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli.

1988 • 1,536 citations

Single-Chain Antigen-Binding Proteins

1988 • 1,450 citations

X-Ray Structure of the GCN4 Leucine Zipper, a Two-Stranded, Parallel Coiled Coil

1991 • 1,433 citations

Canonical structures for the hypervariable regions of immunoglobulins

1987 • 1,357 citations

Molecular cloning (2nd Ed)

1989 • 1,191 citations

Anatomy of the antibody molecule

1994 • 868 citations

Single‐domain antibody fragments with high conformational stability

2002 • 586 citations

β-Hairpin families in globular proteins

1985 • 533 citations

Conformation of β-hairpins in protein structures

1989 • 476 citations

A comparison of strategies to stabilize immunoglobulin Fv-fragments

1990 • 469 citations

Transgenic plants expressing a functional single-chain Fv antibody are specifically protected from virus attack

1993 • 427 citations

Domain association in immunoglobulin molecules

1985 • 389 citations

Ribosome display efficiently selects and evolves high-affinity antibodiesin vitrofrom immune libraries

1998 • 287 citations

Contact with a component of the polymerase II holoenzyme suffices for gene activation

1995 • 262 citations

Antibody engineering: Comparison of bacterial, yeast, insect and mammalian expression systems

1998 • 262 citations

Antibody scFv fragments without disulfide bonds, made by molecular evolution 1 1Edited by I. A. Wilson

1998 • 240 citations

Tailoringin vitroevolution for protein affinity or stability

2000 • 228 citations

Yet Another Numbering Scheme for Immunoglobulin Variable Domains: An Automatic Modeling and Analysis Tool

2001 • 224 citations

Expression of an antibody fragment at high levels in the bacterial cytoplasm

1998 • 213 citations

The disulfide bonds in antibody variable domains: effects on stability, folding in vitro, and functional expression in Escherichia coli

1992 • 190 citations

Antibody Engineering

2000 • 186 citations

Redox State of Single Chain Fv Fragments Targeted to the Endoplasmic Reticulum, Cytosol and Mitochondria

1995 • 182 citations

Selection of antibodies for intracellular function using a two-hybridin vivosystem

1999 • 178 citations

Improving in vivo folding and stability of a single-chain Fv antibody fragment by loop grafting

1997 • 155 citations

The intracellular antibody capture technology (IACT): towards a consensus sequence for intracellular antibodies

2002 • 135 citations

Correlation between in Vitro Stability and in Vivo Performance of Anti-GCN4 Intrabodies as Cytoplasmic Inhibitors

2000 • 134 citations

Gene activation by recruitment of the RNA polymerase II holoenzyme.

1996 • 123 citations

Mutual Stabilization of V_Land V_Hin Single-Chain Antibody Fragments, Investigated with Mutants Engineered for Stability

1998 • 123 citations

Intracellular expression of an antibody fragment-neutralizing p21 ras promotes tumor regression.

1998 • 120 citations

[44] Prokaryotic coupled transcription — translation

1983 • 116 citations

INTRACELLULAR ANTIBODIES (INTRABODIES) FOR GENE THERAPY OF INFECTIOUS DISEASES

1997 • 116 citations

In Vitro Transcription: Preparative RNA Yields in Analytical Scale Reactions

1994 • 99 citations

Intracellular antibody capture technology: application to selection of intracellular antibodies recognising the BCR-ABL oncogenic protein

2002 • 91 citations

Changing Fos oncoprotein to a Jun-independent DNA-binding protein with GCN4 dimerization specificity by swapping "leucine zippers"

1989 • 90 citations

Intracellular Expression of Anti-p21ras Single Chain Fv Fragments Inhibits Meiotic Maturation of Xenopus Oocytes

1993 • 77 citations

Antibody engineering — A practical approach

1997 • 72 citations

Diverting a protein from its cellular location by intracellular antibodies

2000 • 69 citations

Genome-wide RNAi

2001 • 69 citations

Conservation of Glutamine-Rich Transactivation Function between Yeast and Humans

2000 • 67 citations

Fast selection of antibodies without antigen purification: adaptation of the protein fragment complementation assay to select antigen-antibody pairs

2001 • 61 citations

Antigen‐independent selection of stable intracellular single‐chain antibodies

2001 • 58 citations

Transgenic gene knock-outs: functional genomics and therapeutic target selection

2000 • 33 citations

In vivo selection of single-chain antibodies using a yeast two-hybrid system

2000 • 22 citations

Antisense oligonucleotide technology: from EST to therapeutics.

2000 • 12 citations

Immunotherapy of plant viral diseases

1995 • 12 citations

Isolation and characterization of single‐chain Fv genes encoding antibodies specific for Drosophila Poxn protein

1998 • 11 citations

Model of a LexA Repressor Dimer Bound torecA Operator

2000 • 5 citations