Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli.

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Short-Term Effects of Nose-Only Cigarette Smoke Exposure on Glutathione Redox Homeostasis, Cytochrome P450 1A1/2 and Respiratory Enzyme Activities in Mice Tissues

2013 • 47,043 citations

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

1985 • 15,222 citations

The protein data bank: A computer-based archival file for macromolecular structures

1977 • 8,627 citations

Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes.

1987 • 5,066 citations

Rapid analysis of amino acids using pre-column derivatization

1984 • 2,491 citations

The hydrolysis of rabbit γ-globulin and antibodies with crystalline papain

1959 • 2,294 citations

A gas-liquid solid phase peptide and protein sequenator.

1981 • 2,238 citations

[1] Measurement of molecular weights by electrophoresis on SDS-acrylamide gel

1972 • 1,733 citations

Replacing the complementarity-determining regions in a human antibody with those from a mouse

1986 • 1,356 citations

The Protein Data Bank

1977 • 716 citations

Numbers of Receptor Sites from Scatchard Graphs: Facts and Fantasies

1982 • 683 citations

Phosphocholine binding immunoglobulin Fab McPC603

1986 • 576 citations

Crystallographic refinement and atomic models of the intact immunoglobulin molecule Kol and its antigen-binding fragment at 3.0 Å and 1.9 Å resolution

1980 • 455 citations

Prediction of the folding of short polypeptide segments by uniform conformational sampling

1987 • 428 citations

Codon frequencies in 119 individual genes confirm corsistent choices of degenerate bases according to genome type

1980 • 370 citations

Preliminary refinement and structural analysis of the Fab fragment from human immunoglobulin new at 2.0 A resolution.

1978 • 293 citations

Anomalous cleavage of aspartyl-proline peptide bonds during amino acid sequence determinations

1970 • 293 citations

Analysis of the repertoire of anti‐(4‐hydroxy‐3‐nitro‐phenyl)acetyl (NP) antibodies in C 57 BL/6 mice by cell fusion. II. Characterization of idiotopes by monoclonal anti‐idiotope antibodies

1979 • 254 citations

Characterization of antibodies of high affinity and specificity for the digitalis glycoside digoxin

1970 • 245 citations

Generation of antibody activity from immunoglobulin polypeptide chains produced in Escherichia coli.

1984 • 167 citations

Assembly of functional antibodies from immunoglobulin heavy and light chains synthesised inE. coli

1984 • 165 citations

Localization of Antibody-Combining Sites within the Variable Portions of Heavy and Light Chains

1972 • 162 citations

Active antibody fragment (Fv) composed of the variable portions of heavy and light chains

1973 • 151 citations

The synthesis and in vivo assembly of functional antibodies in yeast

1985 • 145 citations

Chemical synthesis and expression of a calmodulin gene designed for site-specific mutagenesis

1985 • 140 citations

Immunogenicity and kinetics of distribution and elimination of sheep digoxin-specific IgG and Fab fragments in the rabbit and baboon.

1979 • 134 citations

Translation of the leader region of the Escherichia coli tryptophan operon

1978 • 125 citations

RECOVERY OF ANTIGENIC SPECIFICITY AFTER DENATURATION AND COMPLETE REDUCTION OF DISULFIDES IN A PAPAIN FRAGMENT OF ANTIBODY

1964 • 124 citations

High-affinity monoclonal antibodies to the cardiac glycoside, digoxin.

1982 • 120 citations

Binding and structural diversity among high-affinity monoclonal anti-digoxin antibodies

1985 • 87 citations

RECOVERY OF SPECIFIC ACTIVITY AFTER COMPLETE UNFOLDING AND REDUCTION OF AN ANTIBODY FRAGMENT

1965 • 80 citations

Specific cleavage between variable and constant domains of rabbit antibody light chains by dilute acid hydrolysis

1972 • 69 citations

Variable region framework differences result in decreased or increased affinity of variant anti-digoxin antibodies.

1988 • 59 citations

Preparation of Fv fragment from the mouse myeloma XRPC-25 immunoglobulin possessing antidinitrophenyl activity

1976 • 56 citations

Site-directed mutagenesis of an invariant amino acid residue at the variable-diversity segments junction of an antibody.

1986 • 55 citations

Folding and interaction of subunits at the antibody combining site

1976 • 45 citations

Cold pepsin digestion: a novel method to produce the Fv fragment from human immunoglobulin M.

1978 • 43 citations

An Active Derivative of Rabbit Antibody Light Chain Composed of the Constant and the Variable Domains Held Together Only by a Native Disulfide Bond

1972 • 37 citations

Proteolytic dissection of a hapten binding site

1986 • 23 citations

Immunoglobulin chain recombination among antidigoxin antibodies by hybridoma-hybridoma fusion.

1987 • 20 citations

Characterization of the Fv Fragment Isolated from a Human Immunoglobulin M

1974 • 20 citations

Properties of the Fd fragment from rabbit immunoglobulin G

1972 • 14 citations