In vivo selection of single-chain antibodies using a yeast two-hybrid system

A novel genetic system to detect protein–protein interactions

1989 • 6,022 citations

Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.

1985 • 2,456 citations

High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier

1989 • 2,140 citations

Mammalian Ras interacts directly with the serine/threonine kinase raf

1993 • 1,983 citations

Oxidized Redox State of Glutathione in the Endoplasmic Reticulum

1992 • 1,909 citations

By-passing immunization

1991 • 1,603 citations

Cdi1, a human G1 and S phase protein phosphatase that associates with Cdk2

1993 • 1,531 citations

The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest.

1991 • 1,489 citations

Multi-subunit proteins on the surface of filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains

1991 • 1,014 citations

A novel genetic system to detect protein-protein interactions

1991 • 532 citations

Leucine zipper structure of the protein CRE-BP1 binding to the cyclic AMP response element in brain.

1989 • 396 citations

Antibody scFv fragments without disulfide bonds, made by molecular evolution 1 1Edited by I. A. Wilson

1998 • 240 citations

Expression of an antibody fragment at high levels in the bacterial cytoplasm

1998 • 213 citations

Novel vectors for the expression of antibody molecules using variable regions generated by polymerase chain reaction

1992 • 180 citations

Distinct activation domains within cAMP response element-binding protein (CREB) mediate basal and cAMP-stimulated transcription

1993 • 166 citations

Intracellular expression of an antibody fragment-neutralizing p21 ras promotes tumor regression.

1998 • 120 citations

INTRACELLULAR ANTIBODIES (INTRABODIES) FOR GENE THERAPY OF INFECTIOUS DISEASES

1997 • 116 citations

Single-step purification of shuttle vectors from yeast for high frequency back-transformation intoE. coli

1990 • 105 citations

Phenotypic knockout of the high-affinity human interleukin 2 receptor by intracellular single-chain antibodies against the alpha subunit of the receptor.

1995 • 96 citations

Ras-Specific Exchange Factor GRF: Oligomerization through Its Dbl Homology Domain and Calcium-Dependent Activation of Raf

1999 • 88 citations

A Multifunctional Prokaryotic Protein Expression System: Overproduction, Affinity Purification, and Selective Detection

1993 • 83 citations

An improved method for generating single-chain antibodies from hybridomas

1993 • 50 citations

Primer design for the cloning of immunoglobulin heavy-chain leader-variable regions from mouse hybridoma cells using the PCR.

1991 • 46 citations