Topology of eukaryotic type II membrane proteins: Importance of N-terminal positively charged residues flanking the hydrophobic domain

Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.

1975 • 3,026 citations

Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase.

1986 • 2,118 citations

Intracellular protein topogenesis

1980 • 1,378 citations

The spontaneous insertion of proteins into and across membranes: The helical hairpin hypothesis

1981 • 868 citations

The distribution of positively charged residues in bacterial inner membrane proteins correlates with the trans-membrane topology

1986 • 861 citations

Topogenic signals in integral membrane proteins

1988 • 715 citations

Selective solubilization of proteins from red blood cell membranes by protein perturbants

1973 • 611 citations

Predicting the orientation of eukaryotic membrane-spanning proteins.

1989 • 596 citations

Influenza virus M2 protein is an integral membrane protein expressed on the infected-cell surface

1985 • 579 citations

Mechanism of Protein Translocation Across the Endoplasmic Reticulum Membrane

1986 • 509 citations

Transcending the impenetrable: How proteins come to terms with membranes

1988 • 471 citations

Secretion and Membrane Localization of Proteins inEscherichia Coli

1980 • 402 citations

Trans‐membrane Translocation of Proteins

1979 • 364 citations

Primary structure of human transferrin receptor deduced from the mRNA sequence

1984 • 351 citations

Purification of microsomal signal peptidase as a complex.

1986 • 313 citations

Translocation of secretory proteins across the microsomal membrane occurs through an environment accessible to aqueous perturbants

1985 • 277 citations

Early events in the biosynthesis of the lysosomal enzyme cathepsin D.

1979 • 233 citations

SecA interacts with secretory proteins by recognizing the positive charge at the amino terminus of the signal peptide in Escherichia coli.

1990 • 229 citations

Evidence for a ninth influenza viral polypeptide

1978 • 222 citations

Fine-tuning the topology of a polytopic membrane protein: Role of positively and negatively charged amino acids

1990 • 221 citations

Sequence of a second human asialoglycoprotein receptor: conservation of two receptor genes during evolution.

1985 • 182 citations

Intracellular maturation and transport of the SV5 type II glycoprotein hemagglutinin-neuraminidase: specific and transient association with GRP78-BiP in the endoplasmic reticulum and extensive internalization from the cell surface.

1989 • 159 citations

Synthesis of influenza virus proteins in infected cells: Translation of viral polypeptides, including three P polypeptides, from RNA produced by primary transcription

1976 • 153 citations

Evidence for the loop model of signal-sequence insertion into the endoplasmic reticulum.

1988 • 148 citations

The membrane-spanning segment of invariant chain (Iγ) contains a potentially cleavable signal sequence

1986 • 142 citations

Towards a comparative anatomy of N-terminal topogenic protein sequences

1986 • 140 citations

Positive charges at the NH2 terminus convert the membrane-anchor signal peptide of cytochrome P-450 to a secretory signal peptide.

1988 • 135 citations

Hemagglutinin-neuraminidase protein of the paramyxovirus simian virus 5: nucleotide sequence of the mRNA predicts an N-terminal membrane anchor

1985 • 132 citations

Expression at the cell surface of biologically active fusion and hemagglutinin/neuraminidase proteins of the paramyxovirus simian virus 5 from cloned cDNA.

1985 • 132 citations

The complete sequence of the mRNA for the HLA-DR-associated invariant chain reveals a polypeptide with an unusual transmembrane polarity.

1984 • 124 citations

Assembly of translocation-competent proteoliposomes from detergent-solubilized rough microsomes

1990 • 118 citations

Different roles of individual N-linked oligosaccharide chains in folding, assembly, and transport of the simian virus 5 hemagglutinin-neuraminidase.

1990 • 116 citations

Conservation of the influenza virus membrane protein (M1) amino acid sequence and an open reading frame of RNA segment 7 encoding a second protein (M2) in H1N1 and H3N2 strains

1981 • 99 citations

A tripartite structure of the signals that determine protein insertion into the endoplasmic reticulum membrane.

1989 • 98 citations

Incorporation of a charged amino acid into the membrane-spanning domain blocks cell surface transport but not membrane anchoring of a viral glycoprotein.

1985 • 93 citations

Identification and predicted sequence of a previously unrecognized small hydrophobic protein, SH, of the paramyxovirus simian virus 5

1985 • 90 citations

The amino-terminal structures that determine topological orientation of cytochrome P-450 in microsomal membrane.

1990 • 90 citations

Access of proteinase K to partially translocated nascent polypeptides in intact and detergent-solubilized membranes.

1989 • 88 citations

Spliced and unspliced messenger RNAs synthesized from cloned influenza virus M DNA in an SV40 vector: Expression of the influenza virus membrane protein (M1)

1982 • 85 citations

Determination of the orientation of an integral membrane protein and sites of glycosylation by oligonucleotide-directed mutagenesis: influenza B virus NB glycoprotein lacks a cleavable signal sequence and has an extracellular NH2-terminal region.

1986 • 84 citations

Charge clusters and the orientation of membrane proteins

1982 • 82 citations

Integration of a small integral membrane protein, M2, of influenza virus into the endoplasmic reticulum: analysis of the internal signal-anchor domain of a protein with an ectoplasmic NH2 terminus.

1988 • 77 citations

Nascent secretory chain binding and translocation are distinct processes: differentiation by chemical alkylation.

1989 • 75 citations

Foreign transmembrane peptides replacing the internal signal sequence of transferrin receptor allow its translocation and membrane binding

1987 • 75 citations

Signal recognition particle-dependent membrane insertion of mouse invariant chain: a membrane-spanning protein with a cytoplasmically exposed amino terminus.

1986 • 69 citations

Human erythrocyte membrane sialoglycoprotein β. The cDNA sequence suggests the absence of a cleaved N-terminal signal sequence

1987 • 54 citations

Orientation of the v-erb-B gene product in the plasma membrane.

1986 • 34 citations

Transposition of domains between the M2 and HN viral membrane proteins results in polypeptides which can adopt more than one membrane orientation.

1989 • 27 citations

Cited By (0)

Loading...