Multiple Determinants Direct the Orientation of Signal–Anchor Proteins: The Topogenic Role of the Hydrophobic Signal Domain

A simple method for displaying the hydropathic character of a protein

1982 • 22,471 citations

Replacement of insulin receptor tyrosine residues 1162 and 1163 compromises insulin-stimulated kinase activity and uptake of 2-deoxyglucose

1986 • 1,100 citations

The distribution of positively charged residues in bacterial inner membrane proteins correlates with the trans-membrane topology

1986 • 861 citations

[71] Use of eukaryotic expression technology in the functional analysis of cloned genes

1987 • 834 citations

Signal Sequence Recognition and Protein Targeting to the Endoplasmic Reticulum Membrane

1994 • 821 citations

Topogenic signals in integral membrane proteins

1988 • 715 citations

Predicting the orientation of eukaryotic membrane-spanning proteins.

1989 • 596 citations

Control of topology and mode of assembly of a polytopic membrane protein by positively charged residues

1989 • 587 citations

An investigation of the role of transmembrane domains in Golgi protein retention.

1995 • 384 citations

Membrane Proteins: From Sequence to Structure

1994 • 298 citations

Translocation of secretory proteins across the microsomal membrane occurs through an environment accessible to aqueous perturbants

1985 • 277 citations

An internal signal sequence: The asialoglycoprotein receptor membrane anchor

1986 • 209 citations

Signal sequence-dependent function of the TRAM protein during early phases of protein transport across the endoplasmic reticulum membrane.

1996 • 182 citations

Sequence of human asialoglycoprotein receptor cDNA. An internal signal sequence for membrane insertion.

1985 • 182 citations

Functions of signal and signal-anchor sequences are determined by the balance between the hydrophobic segment and the N-terminal charge.

1992 • 180 citations

Topology of eukaryotic type II membrane proteins: Importance of N-terminal positively charged residues flanking the hydrophobic domain

1991 • 153 citations

Signals for the incorporation and orientation of cytochrome P450 in the endoplasmic reticulum membrane.

1988 • 151 citations

The COOH-terminal ends of internal signal and signal-anchor sequences are positioned differently in the ER translocase.

1994 • 146 citations

Charged residues are major determinants of the transmembrane orientation of a signal-anchor sequence.

1991 • 144 citations

Membrane proteins: from sequence to structure

1990 • 137 citations

Positive charges at the NH2 terminus convert the membrane-anchor signal peptide of cytochrome P-450 to a secretory signal peptide.

1988 • 135 citations

Positively charged residues are important determinants of membrane protein topology

1990 • 132 citations

Heads or tails — what determines the orientation of proteins in the membrane

1995 • 125 citations

Transmembrane orientation of signal-anchor proteins is affected by the folding state but not the size of the N-terminal domain.

1995 • 123 citations

The amino-terminal structures that determine topological orientation of cytochrome P-450 in microsomal membrane.

1990 • 90 citations

NH2-terminal substitutions of basic amino acids induce translocation across the microsomal membrane and glycosylation of rabbit cytochrome P450IIC2.

1989 • 73 citations

The role of the N region in signal sequence and signal-anchor function.

1992 • 65 citations

Deletion of the amino-terminal domain of asialoglycoprotein receptor H1 allows cleavage of the internal signal sequence.

1988 • 57 citations

Role of NH2-terminal positively charged residues in establishing membrane protein topology.

1993 • 57 citations

Deletion analysis of the internal signal-anchor domain of the human asialoglycoprotein receptor H1.

1987 • 40 citations

trans-Golgi retention of a plasma membrane protein: mutations in the cytoplasmic domain of the asialoglycoprotein receptor subunit H1 result in trans-Golgi retention.

1995 • 18 citations

Differential Effects of Changes in the Length of a Signal/Anchor Domain on Membrane Insertion, Subunit Assembly, and Intracellular Transport of a Type II Integral Membrane Protein

1996 • 7 citations