RNA molecules lighting up under the microscope

Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter

1984 • 6,459 citations

Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization.

1986 • 3,231 citations

Sequence-Selective Recognition of DNA by Strand Displacement with a Thymine-Substituted Polyamide

1991 • 3,211 citations

PNA hybridizes to complementary oligonucleotides obeying the Watson–Crick hydrogen-bonding rules

1993 • 2,034 citations

Detection of mRNAs in sea urchin embryos by in situ hybridization using asymmetric RNA probes

1984 • 1,638 citations

Phylogenetic Stains: Ribosomal RNA-Based Probes for the Identification of Single Cells

1989 • 1,231 citations

Cell Biology: a Laboratory Handbook

1995 • 1,226 citations

Heterogeneity in telomere length of human chromosomes

1996 • 861 citations

Padlock Probes: Circularizing Oligonucleotides for Localized DNA Detection

1994 • 764 citations

Biotin amplification of biotin and horseradish peroxidase signals in histochemical stains.

1992 • 651 citations

Intracellular localization of messenger RNAs for cytoskeletal proteins

1986 • 639 citations

Sensitive mRNA detection using unfixed tissue: combined radioactive and non-radioactive in situ hybridization histochemistry

1992 • 588 citations

The intracellular localization of messenger RNAs

1995 • 534 citations

Role of Chromosome Territories in the Functional Compartmentalization of the Cell Nucleus

1993 • 504 citations

Sequences responsible for intracellular localization of beta-actin messenger RNA also affect cell phenotype.

1994 • 494 citations

Localization of gold in biological tissue

1981 • 465 citations

Highly localized tracks of specific transcripts within interphase nuclei visualized by in situ hybridization

1989 • 434 citations

Quantitative analysis ofin situhybridization methods for the detection of actin gene expression

1985 • 370 citations

Nuclear export of different classes of RNA is mediated by specific factors

1994 • 323 citations

A novel in situ hybridization signal amplification method based on the deposition of biotinylated tyramine.

1995 • 316 citations

Discrete nuclear domains of poly(A) RNA and their relationship to the functional organization of the nucleus.

1991 • 300 citations

Growth-controlling molluscan neurons produce the precursor of an insulin-related peptide

1988 • 288 citations

Ultra-sensitive FISH using peroxidase-mediated deposition of biotin- or fluorochrome tyramides

1995 • 260 citations

Nonrandom gene organization: structural arrangements of specific pre-mRNA transcription and splicing with SC-35 domains.

1995 • 251 citations

Evidence for a nuclear compartment of transcription and splicing located at chromosome domain boundaries

1993 • 241 citations

Detection of poly A⁺RNA in sea urchin eggs and embryos by quantitative in situ hybridization

1981 • 238 citations

Getting the message from the gene to the synapse: sorting and intracellular transport of RNA in neurons

1992 • 235 citations

A nuclear cap-binding complex binds Balbiani ring pre-mRNA cotranscriptionally and accompanies the ribonucleoprotein particle during nuclear export.

1996 • 232 citations

A Pre-mRNA-Binding Protein Accompanies the RNA from the Gene through the Nuclear Pores and into Polysomes

1996 • 220 citations

Non-radioactive labeling of RNA transcriptsin vitrowith the hapten digoxigenin (DIG); hybridization and ELISA-based detection

1990 • 200 citations

Demonstration of local protein synthesis within dendrites using a new cell culture system that permits the isolation of living axons and dendrites from their cell bodies.

1992 • 197 citations

In situhybridisation with fluoresceinated DNA

1991 • 192 citations

Demonstration of local protein synthesis within dendrites using a new cell culture system that permits the isolation of living axons and dendrites from their cell bodies

1992 • 174 citations

In vivo detection of snRNP-rich organelles in the nuclei of mammalian cells.

1991 • 167 citations

A perinucleolar compartment contains several RNA polymerase III transcripts as well as the polypyrimidine tract-binding protein, hnRNP I [published erratum appears in J Cell Biol 1995 Jul;130(2):497-500]

1995 • 164 citations

Axonal transport of neuropeptide encoding mRNAs within the hypothalamo-hypophyseal tract of rats.

1991 • 163 citations

mRNA coding for oxytocin is present in axons of the hypothalamo-neurohypophysial tract.

1990 • 148 citations

Nup120p: a yeast nucleoporin required for NPC distribution and mRNA transport.

1995 • 146 citations

Evidence for channeled diffusion of pre-mRNAs during nuclear RNA transport in metazoans.

1993 • 144 citations

Detection of low copy human papilloma virus DNA and mRNA in routine paraffin sections of cervix by non-isotopic in situ hybridisation.

1987 • 134 citations

Nup145p is required for nuclear export of mRNA and binds homopolymeric RNA in vitro via a novel conserved motif

1994 • 134 citations

Nuclear pore complex clustering and nuclear accumulation of poly(A)+ RNA associated with mutation of the Saccharomyces cerevisiae RAT2/NUP120 gene.

1995 • 133 citations

Comparison of 35S- and digoxigenin-labeled RNA and oligonucleotide probes for in situ hybridization

1992 • 129 citations

Determinants of mRNA localization

1992 • 127 citations

Evolution of the vasopressin/oxytocin superfamily: characterization of a cDNA encoding a vasopressin-related precursor, preproconopressin, from the mollusc Lymnaea stagnalis.

1992 • 122 citations

In situ hybridization demonstration of poly‐adenylated RNA sequences in formalin‐fixed paraffin sections using a biotinylated oligonucleotide poly d(T) probe

1989 • 106 citations

Localisation of cellular globin messenger RNA by in situ hybridisation to complementary DNA

1973 • 98 citations

Improved localization of fluorescent tyramides for fluorescence in situ hybridization using dextran sulfate and polyvinyl alcohol.

1996 • 89 citations

Nucleolar accumulation of poly (A)+ RNA in heat-shocked yeast cells: implication of nucleolar involvement in mRNA transport.

1995 • 88 citations

Chapter 2 Localization of mRNAs by in Situ Hybridization

1991 • 85 citations

Characterization of a cDNA clone encoding multiple copies of the neuropeptide APGWamide in the mollusk Lymnaea stagnalis

1992 • 83 citations

Analysis of antifading reagents for fluorescence microscopy

1995 • 82 citations

Practical aspects of radio-isotopic in situ hybridization on RNA

1993 • 79 citations

Detection of mRNA molecules coding for neuropeptide hormones of the pond snail Lymnaea stagnalis by radioactive and non-radioactive in situ hybridization: a model study for mRNA detection.

1989 • 76 citations

Occurrence and Sequence Complexity of Polyadenylated RNA in Squid Axoplasm

1987 • 71 citations

Optimization of non-radioactive in situ hybridization: image analysis of varying pretreatment, hybridization and probe labelling conditions

1990 • 69 citations

Simultaneous use of digoxigenin- and radiolabeled oligodeoxyribonucleotide probes for hybridization histochemistry

1989 • 69 citations

Methodologies for specific intron and exon RNA localization in cultured cells by haptenized and fluorochromized probes

1993 • 68 citations

Localization of ovulation hormone-like neuropeptide in the central nervous system of the snail Lymnaea stagnalis by means of immunocytochemistry and in situ hybridization

1988 • 67 citations

RNA in the axonal domain: a new dimension in neuronal functioning?

1994 • 64 citations

Bicolor fluorescence in situ hybridization to intron and exon mRNA sequences

1991 • 58 citations

Detection of proopiomelanocortin mRNA by in situ hybridization, using a biotinylated oligodeoxynucleotide probe and avidin-alkaline phosphatase histochemistry

1988 • 58 citations

IN SITU LOCALIZATION OF GLOBIN MESSENGER RNA FORMATION

1974 • 58 citations

Nuclear RNA tracks: structural basis for transcription and splicing?

1993 • 57 citations

Electronic light microscopy: present capabilities and future prospects

1995 • 55 citations

Differential subcellular mRNA targeting: deletion of a single nucleotide prevents the transport to axons but not to dendrites of rat hypothalamic magnocellular neurons.

1995 • 52 citations

CCD microscopy and image analysis of cells and chromosomes stained by fluorescence in situ hybridization

1995 • 52 citations

Enhanced sensitivity for light and electron microscopic in situ hybridization with multiple simultaneous non-radioactive oligodeoxynucleotide probes.

1995 • 52 citations

3′-End fluorochromized and haptenized oligonucleotides as in situ hybridization probes for multiple, simultaneous RNA detection

1991 • 49 citations

Sensitive non-radioisotopic in situ hybridization histochemistry: demonstration of tyrosine hydroxylase gene expression in rat brain and adrenal

1990 • 47 citations

RNAs radiate from gene to cytoplasm as revealed by fluorescence in situ hybridization

1995 • 45 citations

Differential expression in blood stages of the gene coding for the 21-kilodalton surface protein of ookinetes of Plasmodium berghei as detected by RNA in situ hybridisation

1994 • 41 citations

Simultaneous detection of different mRNA sequences coding for neuropeptide hormones by double in situ hybridization using FITC- and biotin-labeled oligonucleotides.

1990 • 40 citations

Light and electron microscopic in situ hybridization: non-radioactive labeling and detection, double hybridization, and combined hybridization-immunocytochemistry.

1994 • 40 citations

Sensitive detection of RNAs in single cells by flow cytometry

1992 • 39 citations

Differential expression of four genes encoding molluscan insulin-related peptides in the central nervous system of the pond snail Lymnaea stagnalis

1992 • 34 citations

One FISH, two FISH, red FISH, blue FISH

1996 • 31 citations

Histological aspects of in situ hybridization

1986 • 31 citations

Detection of parvovirus DNA in human serum using biotinylated RNA hybridisation probes

1988 • 31 citations

Nucleolar accumulation of poly (A)+ RNA in heat-shocked yeast cells: implication of nucleolar involvement in mRNA transport.

1996 • 30 citations

Use of oligodeoxynucleotide probes for quantitative in situ hybridization to actin mRNA

1987 • 28 citations

Ultrastructural localization of epidermal growth factor (EGF)-receptor transcripts in the cell nucleus using pre-embedding in situ hybridization in combination with ultra-small gold probes and silver enhancement

1994 • 28 citations

Axonal localization of neuropeptide-encoding mRNA in identified neurons of the snail Lymnaea stagnalis

1994 • 28 citations

Expression of the egg-laying hormone genes in peripheral neurons and exocrine cells in the reproductive tract of the mollusc Lymnaea stagnalis

1989 • 27 citations

Electron microscopic detection of RNA sequences by non-radioactive in situ hybridization in the mollusk Lymnaea stagnalis.

1992 • 26 citations

Qualitative and quantitative detection of alkaline phosphatase coupled to an oligonucleotide probe for somatostatin mRNA after in situ hybridization using unfixed rat brain tissue

1995 • 24 citations

Ultrastructural evidence for the axonal localization of caudodorsal cell hormone mRNA in the central nervous system of the mollusc Lymnaea stagnalis

1993 • 24 citations

Localization of nuclear RNA by pre- and post-embedding in situ hybridization using different gold probes

1995 • 24 citations

Asymmetric localization of Drosophila pair-rule transcripts from displaced nuclei: evidence for directional nuclear export.

1996 • 22 citations

Localization of tyrosine hydroxylase mRNA in the axons of the hypothalamo-neurohypophysial system

1994 • 22 citations

An evaluation of a new series of fluorescent dUTPs for fluorescence in situ hybridization.

1996 • 19 citations

Saponin pre-treatment in pre-embedding electron microscopic in situ hybridization for detection of specific RNA sequences in cultured cells: a methodological study.

1995 • 18 citations

Oligonucleotide probes detect splicing variants insituinDrosophilaembryos

1992 • 17 citations

Double labelling in situ hybridization using non-isotopic and isotopic detection.

1987 • 13 citations

In situ Hybridization with Hapten-Modified DNA Probes

2015 • 13 citations

Imaging of RNA in situ hybridization by atomic force microscopy

1996 • 12 citations

Nonradioactive detection of vasopressin mRNA with in situ hybridization histochemistry

1989 • 12 citations

Monitoring morphology and signal during non-radioactive in situ hybridization procedures by reflection-contrast microscopy and transmission electron microscopy.

1995 • 12 citations

Cell-cycle-dependent gene expression studied by two-colour fluorescent detection of a mRNA and histone mRNA

1995 • 11 citations

Detection of specific messenger RNA by electron microscopic in situ hybridization: implications for nucleocytoplasmic transport.

1995 • 6 citations

Evaluation of pepsin treatment for electron microscopic RNA in situ hybridization on ultra-thin cryosections of cultured cells

1996 • 6 citations