Non-radioactive labeling of RNA transcriptsin vitrowith the hapten digoxigenin (DIG); hybridization and ELISA-based detection

Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter

1984 • 6,459 citations

Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.

1982 • 2,753 citations

Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates

1987 • 2,098 citations

Detection of mRNAs in sea urchin embryos by in situ hybridization using asymmetric RNA probes

1984 • 1,638 citations

High-Resolution Mapping of Human Chromosome 11 by in Situ Hybridization with Cosmid Clones

1990 • 1,357 citations

Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes.

1981 • 1,165 citations

Evolutionary conservation in the untranslated regions of actin mRNAs: DNA sequence of a human beta-actin cDNA

1984 • 973 citations

The avidin-biotin complex in bioanalytical applications

1988 • 881 citations

Enzyme-Labeling of Antibodies and Their Fragments for Enzyme Immunoassay and Immunohistochemical Staining

1983 • 540 citations

Non-radioactive hybridization probes prepared by the chemical labelling of DNA and RNA with a novel reagent, photobiotin

1985 • 526 citations

Evidence for Trans splicing in trypanosomes

1986 • 486 citations

Injected anti-sense RNAs specifically block messenger RNA translation in vivo.

1985 • 410 citations

1,2‐Dioxetanes: Novel chemiluminescent enzyme substrates. Applications to immunoassays

1989 • 286 citations

High-resolution 1H nuclear magnetic resonance study of cerebral hypoxia in vivo.

1983 • 283 citations

Gene within a gene: Nested Drosophila genes encode unrelated proteins on opposite DNA strands

1986 • 251 citations

Bacteriophage SP6-specific RNA polymerase. I. Isolation and characterization of the enzyme.

1982 • 233 citations

Preparation of capped RNA transcripts using T7 RNA polymerase

1986 • 122 citations

Chemiluminescent detection of DNA: application for DNA sequencing and hybridization

1989 • 113 citations

Metabolic Functions of Biotin

1953 • 93 citations

In situ hybridization with digoxigenin-labeled DNA of human papillomaviruses (HPV 16/18) in HeLa and SiHa cells.

1988 • 84 citations

Cloning and expression of the bacteriophage T3 RNA polymerase gene

1986 • 68 citations

A Method for Biotinylating Oligonucleotide Probes for Use in Molecular Hybridizations

1986 • 60 citations

Sequences of three promoters for the bacteriophage SP6 RNA polymerase

1986 • 58 citations

Purification of Human and Bovine Alkaline Phosphatases by Affinity Chromatography

1980 • 46 citations

Comparison of colorimetric, fluorescent, and enzymatic amplification substrate systems in an enzyme immunoassay for detection of DNA-RNA hybrids

1989 • 42 citations

Determination of avidin and biotin by fluorescence polarization

1988 • 36 citations

Degree of biotinylation in nucleic acids estimated by a gel retardation assay

1989 • 29 citations

VecBase, a cloning vector sequence data base.

1988 • 13 citations