A cGMP-Dependent Protein Kinase Assay for High Throughput Screening Based on Time-Resolved Fluorescence Resonance Energy Transfer

Atherosclerosis — An Inflammatory Disease

1999 • 21,494 citations

A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays

1999 • 6,580 citations

[1] Assays of protein kinase

1983 • 832 citations

Molecular mechanisms and therapeutic strategies related to nitric oxide

1995 • 791 citations

Regulation of cAMP and cGMP signaling: new phosphodiesterases and new functions

2000 • 739 citations

INHIBITION OF NITRIC OXIDE SYNTHASE AS A POTENTIAL THERAPEUTIC TARGET

1999 • 596 citations

Cyclic GMP Phosphodiesterase-5: Target of Sildenafil

1999 • 536 citations

High-throughput screening: new technology for the 21st century

2000 • 530 citations

Defective smooth muscle regulation in cGMP kinase I-deficient mice

1998 • 510 citations

Effects of xanthine derivatives on lipolysis and on adenosine 3',5'-monophosphate phosphodiesterase activity.

1970 • 462 citations

cAMP- and cGMP-dependent protein kinase phosphorylation sites of the focal adhesion vasodilator-stimulated phosphoprotein (VASP) in vitro and in intact human platelets.

1994 • 451 citations

High-throughput and ultra-high-throughput screening: solution- and cell-based approaches

2000 • 450 citations

YC-1, a novel activator of platelet guanylate cyclase

1994 • 401 citations

Distinct and specific functions of cGMP-dependent protein kinases

1997 • 394 citations

Guidebook to the cytoskeletal and motor proteins

1994 • 374 citations

Pharmacology of Protein Kinase Inhibitors

1992 • 367 citations

Rising behind NO: cGMP-dependent protein kinases

2000 • 338 citations

Analysis and Regulation of Vasodilator-stimulated Phosphoprotein Serine 239 Phosphorylation in Vitro and in Intact Cells Using a Phosphospecific Monoclonal Antibody

1998 • 333 citations

The graphical determination of Km and Ki

1972 • 290 citations

Vasodilator-Stimulated Phosphoprotein Serine 239 Phosphorylation as a Sensitive Monitor of Defective Nitric Oxide/cGMP Signaling and Endothelial Dysfunction

2000 • 255 citations

Mechanisms of NO/cGMP-Dependent Vasorelaxation

2000 • 241 citations

Effects of Long-Term Nitroglycerin Treatment on Endothelial Nitric Oxide Synthase (NOS III) Gene Expression, NOS III–Mediated Superoxide Production, and Vascular NO Bioavailability

2000 • 237 citations

Increased Adhesion and Aggregation of Platelets Lacking Cyclic Guanosine 3′,5′-Monophosphate Kinase I

1999 • 228 citations

Guidebook to the Cytoskeletal and Motor Proteins

1999 • 209 citations

Molecular cloning, structural analysis and functional expression of the proline-rich focal adhesion and microfilament-associated protein VASP.

1995 • 208 citations

Downregulation of Soluble Guanylyl Cyclase in Young and Aging Spontaneously Hypertensive Rats

1999 • 205 citations

Homogeneous fluorescence readouts for miniaturized high-throughput screening: theory and practice

1999 • 195 citations

Concentration and regulation of cyclic nucleotides, cyclic‐nucleotide‐dependent protein kinases and one of their major substrates in human platelets

1992 • 168 citations

Vasodilator dysfunction in aged spontaneously hypertensive rats: changes in NO synthase III and soluble guanylyl cyclase expression, and in superoxide anion production

1998 • 166 citations

(Rp)-8-pCPT-cGMPS, a novel cGMP-dependent protein kinase inhibitor

1994 • 156 citations

A Microchip-Based Enzyme Assay for Protein Kinase A

1999 • 155 citations

Time-resolved fluorometry: an overview of the labels and core technologies for drug screening applications

1997 • 146 citations

Analysis of the functional role of cGMP-dependent protein kinase in intact human platelets using a specific activator 8-para-chlorophenylthio-cGMP

1992 • 145 citations

Functional analysis of cGMP-dependent protein kinases I and II as mediators of NO/cGMP effects

1998 • 144 citations

Regulation of Human Endothelial Cell Focal Adhesion Sites and Migration by cGMP-dependent Protein Kinase I

2000 • 133 citations

Inhibition of cGMP‐dependent protein kinase by (Rp)‐guanosine 3',5'‐monophosphorothioates

1990 • 130 citations

Endogenous expression of type II cGMP-dependent protein kinase mRNA and protein in rat intestine. Implications for cystic fibrosis transmembrane conductance regulator.

1995 • 127 citations

Homogeneous Proximity Tyrosine Kinase Assays: Scintillation Proximity Assay versus Homogeneous Time-Resolved Fluorescence

1999 • 125 citations

KT5823 Inhibits cGMP-dependent Protein Kinase Activity in Vitro but Not in Intact Human Platelets and Rat Mesangial Cells

2000 • 124 citations

Immunological distinction between guanosine 3':5'-monophosphate-dependent and adenosine 3':5'-monophosphate-dependent protein kinases.

1980 • 121 citations

Testing for endothelial dysfunction

2000 • 117 citations

Smooth muscle cell expression of type I cyclic GMP-dependent protein kinase is suppressed by continuous exposure to nitrovasodilators, theophylline, cyclic GMP, and cyclic AMP.

1997 • 111 citations

[24] Pseudosubstrate-based peptide inhibitors

1991 • 103 citations

Relaxation of pig coronary arteries by new and potent cGMP analogs that selectively activate type I alpha, compared with type I beta, cGMP-dependent protein kinase.

1992 • 96 citations

The Molecular Basis of Hypertension

1999 • 81 citations

Tyrosine kinase assays adapted to homogeneous time-resolved fluorescence

1998 • 79 citations

LANCE™: Homogeneous Assay Platform for HTS

1999 • 73 citations

Signal transduction by cGMP-dependent protein kinases and their emerging roles in the regulation of cell adhesion and gene expression

2005 • 73 citations

A Scintillation Proximity Assay for the Raf/MEK/ERK Kinase Cascade: High-Throughput Screening and Identification of Selective Enzyme Inhibitors

1999 • 68 citations

Measurement of the Protein Tyrosine Kinase Activity of c-src Using Time-Resolved Fluorometry of Europium Chelates

1996 • 59 citations

Expression, purification, and characterization of the cGMP‐dependent protein kinases Iβ and II using the baculovirus system

1995 • 54 citations

A Chemiluminescent Microtiter Plate Assay for Sensitive Detection of Protein Kinase Activity

1997 • 51 citations

Detection of Phosphopeptides by Fluorescence Polarization in the Presence of Cationic Polyamino Acids: Application to Kinase Assays

2000 • 28 citations

High-throughput screening

2000 • 24 citations