Review: Biosynthesis and function of yeast vacuolar proteases

Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway

1980 • 1,808 citations

Isolation and characterization of autophagy‐defective mutants of Saccharomyces cerevisiae

1993 • 1,649 citations

Autophagy in yeast demonstrated with proteinase-deficient mutants and conditions for its induction.

1992 • 1,187 citations

Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases.

1988 • 977 citations

Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants.

1992 • 832 citations

The fungal vacuole: composition, function, and biogenesis

1990 • 758 citations

Early stages in the yeast secretory pathway are required for transport of carboxypeptidase Y to the vacuole

1982 • 579 citations

Isolation of autophagocytosis mutants of Saccharomyces cerevisiae

1994 • 544 citations

The sorting receptor for yeast vacuolar carboxypeptidase Y is encoded by the VPS10 gene

1994 • 500 citations

Ultrastructural analysis of the autophagic process in yeast: detection of autophagosomes and their characterization

1994 • 480 citations

VESICLE-MEDIATED PROTEIN SORTING

1992 • 472 citations

PROTEINASE MUTANTS OF SACCHAROMYCES CEREVISIAE

1977 • 460 citations

Protein sorting in yeast: Mutants defective in vacuole biogenesis mislocalize vacuolar proteins into the late secretory pathway

1986 • 429 citations

Compartmental organization of Golgi-specific protein modification and vacuolar protein sorting events defined in a yeast sec18 (NSF) mutant.

1991 • 415 citations

PEP4 gene of Saccharomyces cerevisiae encodes proteinase A, a vacuolar enzyme required for processing of vacuolar precursors.

1986 • 400 citations

Isolation of yeast mutants defective in protein targeting to the vacuole.

1986 • 394 citations

Aminopeptidase I of Saccharomyces cerevisiae is localized to the vacuole independent of the secretory pathway.

1992 • 371 citations

Protein sorting in yeast: The localization determinant of yeast vacuolar carboxypeptidase Y resides in the propeptide

1987 • 329 citations

Distinct sequence determinants direct intracellular sorting and modification of a yeast vacuolar protease

1987 • 312 citations

The PEP4 Gene Encodes an Aspartyl Protease Implicated in the Posttranslational Regulation of Saccharomyces cerevisiae Vacuolar Hydrolases

1986 • 292 citations

Intracellular sorting and processing of a yeast vacuolar hydrolase: proteinase A propeptide contains vacuolar targeting information.

1988 • 287 citations

Endocytosis and degradation of the yeast uracil permease under adverse conditions

1994 • 266 citations

Endocytosis is required for the growth of vacuolar H(+)-ATPase-defective yeast: identification of six new END genes.

1994 • 258 citations

Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae.

1989 • 258 citations

Cis- and trans-acting functions required for endocytosis of the yeast pheromone receptors

1993 • 239 citations

Lysosomal (Vacuolar) Proteinases of Yeast Are Essential Catalysts for Protein Degradation, Differentiation, and Cell Survival

1989 • 230 citations

Regulated degradation of HMG-CoA reductase, an integral membrane protein of the endoplasmic reticulum, in yeast.

1994 • 229 citations

Three proteolytic systems in the yeast saccharomyces cerevisiae

1991 • 228 citations

Nonselective autophagy of cytosolic enzymes by isolated rat hepatocytes.

1990 • 218 citations

Gene dosage-dependent secretion of yeast vacuolar carboxypeptidase Y.

1986 • 217 citations

PEP4 GENE FUNCTION IS REQUIRED FOR EXPRESSION OF SEVERAL VACUOLAR HYDROLASES IN SACCHAROMYCES CEREVISIAE

1982 • 211 citations

Mutation of a tyrosine localization signal in the cytosolic tail of yeast Kex2 protease disrupts Golgi retention and results in default transport to the vacuole.

1992 • 209 citations

Analysis of two mutated vacuolar proteins reveals a degradation pathway in the endoplasmic reticulum or a related compartment of yeast

1993 • 205 citations

Mutant defective in processing of an enzyme located in the lysosome-like vacuole of Saccharomyces cerevisiae.

1981 • 199 citations

The PEP4 gene encodes an aspartyl protease implicated in the posttranslational regulation of Saccharomyces cerevisiae vacuolar hydrolases.

1986 • 192 citations

Role of Vacuolar Acidification in Protein Sorting and Zymogen Activation: a Genetic Analysis of the Yeast Vacuolar Proton-Translocating ATPase

1990 • 190 citations

The gene for a major exopolyphosphatase of Saccharomyces cerevisiae

1995 • 188 citations

Membrane protein sorting in the yeast secretory pathway: evidence that the vacuole may be the default compartment.

1992 • 179 citations

Regulated import and degradation of a cytosolic protein in the yeast vacuole

1991 • 174 citations

Propeptide of carboxypeptidase Y provides a chaperone-like function as well as inhibition of the enzymatic activity.

1991 • 173 citations

Biosynthesis of the Vacuolar Yeast Glycoprotein Carboxypeptidase Y

1978 • 164 citations

Two yeast mutants defective in endocytosis are defective in pheromone response

1986 • 164 citations

Protease B of the lysosomelike vacuole of the yeast Saccharomyces cerevisiae is homologous to the subtilisin family of serine proteases.

1987 • 161 citations

Macromolecule Synthesis and Breakdown in Relation to Sporulation and Meiosis in Yeast

1974 • 154 citations

Yeast vacuolar proenzymes are sorted in the late Golgi complex and transported to the vacuole via a prevacuolar endosome-like compartment.

1993 • 152 citations

Structure, biosynthesis, and localization of dipeptidyl aminopeptidase B, an integral membrane glycoprotein of the yeast vacuole.

1989 • 152 citations

Overproduction-induced mislocalization of a yeast vacuolar protein allows isolation of its structural gene.

1986 • 151 citations

Yeast carboxypeptidase Y vacuolar targeting signal is defined by four propeptide amino acids.

1990 • 147 citations

In vivo function of the proteasome in the ubiquitin pathway.

1992 • 144 citations

Direct evidence for ligand-induced internalization of the yeast alpha-factor pheromone receptor.

1994 • 142 citations

Activities and cellular localization of yeast proteases and their inhibitors

1974 • 132 citations

Identification of a novel sequence mediating regulated endocytosis of the G protein-coupled alpha-pheromone receptor in yeast.

1993 • 126 citations

A novel pathway of import of alpha-mannosidase, a marker enzyme of vacuolar membrane, in Saccharomyces cerevisiae.

1990 • 125 citations

Proteolytic activities in yeast

1975 • 124 citations

Partial purification and characterization of early and late endosomes from yeast. Identification of four novel proteins

1993 • 120 citations

PROTEIN DEGRADATION, MEIOSIS AND SPORULATION IN PROTEINASE-DEFICIENT MUTANTS OF SACCHAROMYCES CEREVISIAE

1981 • 115 citations

Detection of an intermediate compartment involved in transport of alpha-factor from the plasma membrane to the vacuole in yeast.

1990 • 111 citations

Biogenesis of the Vacuole in Saccharomyces cerevisiae

1992 • 108 citations

Stress‐induced proteolysis in yeast

1992 • 103 citations

Metabolic instability and constitutive endocytosis of STE6, the a-factor transporter of Saccharomyces cerevisiae.

1994 • 102 citations

Vacuolar/lysosomal proteolysis: proteases, substrates mechanisms

1993 • 97 citations

Role of vacuolar acidification in protein sorting and zymogen activation: a genetic analysis of the yeast vacuolar proton-translocating ATPase.

1990 • 96 citations

Sorting of membrane proteins in the yeast secretory pathway.

1994 • 95 citations

GENETIC PROPERTIES OF MUTATIONS AT THE PEP4 LOCUS IN SACCHAROMYCES CEREVISIAE

1982 • 93 citations

In vivo biosynthesis of the vacuolar proteinases A and B in the yeast Saccharomyces cerevisiae.

1982 • 92 citations

Protease B of Saccharomyces cerevisiae: Isolation and Regulation of the PRB1 Structural Gene

1987 • 90 citations

Protein Degradation and Proteinases during Yeast Sporulation

1976 • 89 citations

Tracing intracellular proteolytic pathways. Proteolysis of fatty acid synthase and other cytoplasmic proteins in the yeast Saccharomyces cerevisiae.

1993 • 87 citations

Maturation of vacuolar (lysosomal) enzymes in yeast: proteinase yscA and proteinase yscB are catalysts of the processing and activation event of carboxypeptidase yscY.

1987 • 85 citations

The propeptide is required for in vivo formation of stable active yeast proteinase A and can function even when not covalently linked to the mature region.

1993 • 84 citations

Isolation and characterization of aminopeptidase mutants of Saccharomyces cerevisiae

1983 • 83 citations

Cellular proteolytic systems

1995 • 83 citations

Yeast carboxypeptidase Y requires glycosylation for efficient intracellular transport, but not for vacuolar sorting, in vivo stability, or activity

1991 • 83 citations

Processing pathway for protease B of Saccharomyces cerevisiae.

1989 • 81 citations

Regulation of Inositol Transport in Saccharomyces cerevisiae Involves Inositol-induced Changes in Permease Stability and Endocytic Degradation in the Vacuole

1995 • 78 citations

Catabolite inactivation of fructose‐1,6‐bisphosphatase in yeast is mediated by the proteasome

1994 • 77 citations

Proteolysis in eukaryotic cells. Proteinase yscE, a new yeast peptidase.

1984 • 76 citations

Effects of glucose and nitrogen source on the levels of proteinases, peptidases, and proteinase inhibitors in yeast

1977 • 76 citations

Autoactivation of proteinase A initiates activation of yeast vacuolar zymogens

1992 • 74 citations

Molecular Cloning and Sequencing of Genomic DNA Encoding Aminopeptidase I from Saccharomyces cerevisiae

1989 • 68 citations

Yeast endocytosis

1993 • 66 citations

Biogenesis of the yeast vacuole (lysosome)

1992 • 63 citations

Activation of the proteinase B precursor of the yeast Saccharomyces cerevisiae by autocatalysis and by an internal sequence.

1991 • 60 citations

Biogenesis of the yeast vacuole (lysosome). The precursor forms of the soluble hydrolase carboxypeptidase yscS are associated with the vacuolar membrane.

1992 • 60 citations

Aminopeptidase Y, a new aminopeptidase from Saccharomyces cerevisiae. Purification, properties, localization, and processing by protease B.

1994 • 59 citations

Analysis of Proteinase A Function in Yeast

1981 • 58 citations

Biogenesis of the yeast lysosome (vacuole): biosynthesis and maturation of proteinase yscB.

1988 • 55 citations

Requirement of the propeptide for in vivo formation of active yeast carboxypeptidase Y.

1994 • 54 citations

pH‐dependent processing of yeast procarboxypeptidase Y by proteinase A in vivo and in vitro

1994 • 48 citations

Phenotypic analysis of proteinase A mutants. Implications for autoactivation and the maturation pathway of the vacuolar hydrolases of Saccharomyces cerevisiae.

1993 • 44 citations

Invivo biosynthesis of vacuolar proteinases in proteinase mutants of Saccharomycescerevisiae

1982 • 42 citations

Consequences of growth media, gene copy number, and regulatory mutations on the expression of the PRB1 gene of Saccharomyces cerevisiae.

1990 • 42 citations

Biogenesis of the yeast vacuole (lysosome)

1992 • 42 citations

Aminopeptidase Co, a new yeast peptidase

1982 • 42 citations

Carboxypeptidase yscS: gene structure and function of the vacuolar enzyme

1991 • 40 citations

The substrate specificity of proteinase B from Baker's yeast

1981 • 34 citations

Molecular cloning of the aminopeptidase Y gene of Saccharomyces cerevisiae. Sequence analysis and gene disruption of a new aminopeptidase.

1994 • 32 citations

Random Substitution of Large Parts of the Propeptide of Yeast Proteinase A

1995 • 28 citations

Substrate specificity of proteinase yscA from Saccharomyces cerevisiae

1989 • 27 citations

Biogenesis of the yeast vacuole (lysosome) Active site mutation in the vacuolar aspartate proteinase yscA blocks maturation of vacuolar proteinases

1991 • 24 citations

The genetic interaction of kar2 and wbp1 mutations. Distinct functions of binding protein BiP and N-linked glycosylation in the processing pathway of secreted proteins in Saccharomyces cerevisiae

1994 • 23 citations

Vacuolar (lysosomal) trehalase ofSaccharomyces cerevisiae

1987 • 23 citations

Molecular cloning and sequencing of genomic DNA encoding yeast vacuolar carboxypeptidase yscS

1991 • 21 citations

Site of catabolite inactivation

1994 • 18 citations

Characterization of proteinase A glycoforms from recombinant Saccharomyces cerevisiae.“

1993 • 14 citations

Transcriptional regulation of the yeast vacuolar aminopeptidase yscI encoding gene (APE1) by carbon sources

1995 • 10 citations

Cis and trans-acting regulatory elements required for regulation of the CPS1 gene in Saccharomyces cerevisiae

1995 • 9 citations

Cellular proteases and control mechanisms

1988 • 4 citations

N‐linked glycosylation of proteinase B precursors of the yeast Saccharomyces cerevisiae is not require for proper targeting or processing of the enzyme

1992 • 2 citations