Biogenesis of the yeast vacuole (lysosome)

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

1979 • 54,907 citations

Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polycrylamide to nitrocellulose

1984 • 2,553 citations

Improved technique utilizing nonfat dry milk for analysis of proteins and nucleic acids transferred to nitrocellulose

1984 • 1,697 citations

The fungal vacuole: composition, function, and biogenesis

1990 • 758 citations

Early stages in the yeast secretory pathway are required for transport of carboxypeptidase Y to the vacuole

1982 • 579 citations

A general method for polyethylene-glycol-induced genetic transformation of bacteria and yeast

1983 • 462 citations

PROTEINASE MUTANTS OF SACCHAROMYCES CEREVISIAE

1977 • 460 citations

PEP4 gene of Saccharomyces cerevisiae encodes proteinase A, a vacuolar enzyme required for processing of vacuolar precursors.

1986 • 400 citations

Membrane protein sorting: biosynthesis, transport and processing of yeast vacuolar alkaline phosphatase.

1989 • 330 citations

Protein sorting in yeast: The localization determinant of yeast vacuolar carboxypeptidase Y resides in the propeptide

1987 • 329 citations

The PEP4 Gene Encodes an Aspartyl Protease Implicated in the Posttranslational Regulation of Saccharomyces cerevisiae Vacuolar Hydrolases

1986 • 292 citations

Intracellular sorting and processing of a yeast vacuolar hydrolase: proteinase A propeptide contains vacuolar targeting information.

1988 • 287 citations

Protein Localization and Membrane Traffic in Yeast

1985 • 267 citations

The vacuole as the lysosome of the yeast cell

1967 • 263 citations

Lysosomal (Vacuolar) Proteinases of Yeast Are Essential Catalysts for Protein Degradation, Differentiation, and Cell Survival

1989 • 230 citations

Three proteolytic systems in the yeast saccharomyces cerevisiae

1991 • 228 citations

PEP4 GENE FUNCTION IS REQUIRED FOR EXPRESSION OF SEVERAL VACUOLAR HYDROLASES IN SACCHAROMYCES CEREVISIAE

1982 • 211 citations

Vacuoles: The sole compartments of digestive enzymes in yeast (Saccharomyces cerevisiae)?

1979 • 202 citations

Mutant defective in processing of an enzyme located in the lysosome-like vacuole of Saccharomyces cerevisiae.

1981 • 199 citations

The PEP4 gene encodes an aspartyl protease implicated in the posttranslational regulation of Saccharomyces cerevisiae vacuolar hydrolases.

1986 • 192 citations

Role of Vacuolar Acidification in Protein Sorting and Zymogen Activation: a Genetic Analysis of the Yeast Vacuolar Proton-Translocating ATPase

1990 • 190 citations

Biosynthesis of the Vacuolar Yeast Glycoprotein Carboxypeptidase Y

1978 • 164 citations

Protease B of the lysosomelike vacuole of the yeast Saccharomyces cerevisiae is homologous to the subtilisin family of serine proteases.

1987 • 161 citations

Overproduction-induced mislocalization of a yeast vacuolar protein allows isolation of its structural gene.

1986 • 151 citations

The sequence of theSaccharomyces cerevisiaegenePH02codes for a regulatory protein with unusual aminoacid composition

1987 • 134 citations

Roles of the VMA3 gene product, subunit c of the vacuolar membrane H(+)-ATPase on vacuolar acidification and protein transport. A study with VMA3-disrupted mutants of Saccharomyces cerevisiae.

1990 • 131 citations

Proteolytic activities in yeast

1975 • 124 citations

PROTEIN DEGRADATION, MEIOSIS AND SPORULATION IN PROTEINASE-DEFICIENT MUTANTS OF SACCHAROMYCES CEREVISIAE

1981 • 115 citations

Comparisons of the Tryptophan Synthase Inactivating Enzymes with Proteinases from Yeast

1974 • 110 citations

Isolation and Properties of Two Inhibitors of Proteinase B from Yeast

1974 • 102 citations

Role of vacuolar acidification in protein sorting and zymogen activation: a genetic analysis of the yeast vacuolar proton-translocating ATPase.

1990 • 96 citations

α-d-mannosidase of Saccharomyces cerevisiae Characterization and modulation of activity

1978 • 96 citations

GENETIC PROPERTIES OF MUTATIONS AT THE PEP4 LOCUS IN SACCHAROMYCES CEREVISIAE

1982 • 93 citations

In vivo biosynthesis of the vacuolar proteinases A and B in the yeast Saccharomyces cerevisiae.

1982 • 92 citations

Protease B of Saccharomyces cerevisiae: Isolation and Regulation of the PRB1 Structural Gene

1987 • 90 citations

Maturation of vacuolar (lysosomal) enzymes in yeast: proteinase yscA and proteinase yscB are catalysts of the processing and activation event of carboxypeptidase yscY.

1987 • 85 citations

Purification and Characterization of Macromolecular Inhibitors of Proteinase A from Yeast

1974 • 84 citations

Processing pathway for protease B of Saccharomyces cerevisiae.

1989 • 81 citations

Effects of glucose and nitrogen source on the levels of proteinases, peptidases, and proteinase inhibitors in yeast

1977 • 76 citations

Molecular and cell biology of yeasts

1989 • 74 citations

Septum formation, cell division, and sporulation in mutants of yeast deficient in proteinase B.

1979 • 73 citations

Studies on a Proteinase B Mutant of Yeast

1979 • 68 citations

Biogenesis of the yeast vacuole (lysosome)

1992 • 63 citations

Activation of the proteinase B precursor of the yeast Saccharomyces cerevisiae by autocatalysis and by an internal sequence.

1991 • 60 citations

Biogenesis of the yeast vacuole (lysosome). The precursor forms of the soluble hydrolase carboxypeptidase yscS are associated with the vacuolar membrane.

1992 • 60 citations

Analysis of Proteinase A Function in Yeast

1981 • 58 citations

Biogenesis of the yeast lysosome (vacuole): biosynthesis and maturation of proteinase yscB.

1988 • 55 citations

Purification and properties of proteinase B from yeast

1981 • 53 citations

Invivo biosynthesis of vacuolar proteinases in proteinase mutants of Saccharomycescerevisiae

1982 • 42 citations

Biogenesis of the yeast vacuole (lysosome) Active site mutation in the vacuolar aspartate proteinase yscA blocks maturation of vacuolar proteinases

1991 • 24 citations

Formation of a Complex between Yeast Proteinases A and B

1975 • 18 citations