Quantitative interpretations of double mutations of enzymes

Hydrogen bonding and biological specificity analysed by protein engineering

1985 • 1,050 citations

A Schematic Method of Deriving the Rate Laws for Enzyme-Catalyzed Reactions

1956 • 994 citations

Redesign of the coenzyme specificity of a dehydrogenase by protein engineering

1990 • 727 citations

Additivity of mutational effects in proteins

1990 • 652 citations

Dissecting the catalytic triad of a serine protease

1988 • 621 citations

The use of double mutants to detect structural changes in the active site of the tyrosyl-tRNA synthetase (Bacillus stearothermophilus)

1984 • 563 citations

Tinkering with Enzymes: What Are We Learning?

1987 • 304 citations

The Catalytic Role of the Active Site Aspartic Acid in Serine Proteases

1987 • 296 citations

Subtilisin — an enzyme designed to be engineered

1988 • 278 citations

EFFECTS OF SITE-SPECIFIC AMINO ACID MODIFICATION ON PROTEIN INTERACTIONS AND BIOLOGICAL FUNCTION

1985 • 224 citations

The Three-Dimensional Structure of Asn ¹⁰² Mutant of Trypsin: Role of Asp ¹⁰² in Serine Protease Catalysis

1987 • 212 citations

Strategies and Applications of in Vitro Mutagenesis

1985 • 192 citations

Tyrosyl-tRNA synthetase forms a mononucleotide-binding fold

1982 • 149 citations

DIRECTED MUTAGENESIS

1981 • 142 citations

Crystallographic analysis of the binding of NADPH, NADPH fragments, and NADPH analogues to glutathione reductase

1988 • 130 citations

Kinetic and magnetic resonance studies of effects of genetic substitution of a calcium-liganding amino acid in staphylococcal nuclease

1986 • 122 citations

Kinetic and ultraviolet spectroscopic studies of active-site mutants of .DELTA.5-3-ketosteroid isomerase

1989 • 122 citations

Site-directed mutagenesis shows that tyrosine 248 of carboxypeptidase A does not play a crucial role in catalysis

1985 • 117 citations

Site-directed mutants of staphylococcal nuclease. Detection and localization by proton NMR spectroscopy of conformational changes accompanying substitutions for glutamic acid-43

1987 • 92 citations

Enzyme-catalyzed allylic rearrangements

1990 • 89 citations

Probing histidine-substrate interactions in tyrosyl-tRNA synthetase using asparagine and glutamine replacements

1985 • 88 citations

Functional interaction among catalytic residues in subtilisin BPN′

1990 • 83 citations

The Δ5-3-ketosteroid isomerase reaction: catalytic mechanism, specificity and inhibition

1976 • 72 citations

MULTIPLE ISOTOPE EFFECTS ON ENZYME-CATALYZED REACTIONS

1989 • 65 citations

Staphylococcal nuclease reviewed: A prototypic study in contemporary enzymology

1979 • 65 citations

Interactions of the acid and base catalysts on staphylococcal nuclease as studied in a double mutant

1991 • 64 citations

Natural variation of tyrosyl-tRNA synthetase and comparison with engineered mutants

1986 • 60 citations

Combined effects of two mutations of catalytic residues on the ketosteroid isomerase reaction

1990 • 59 citations

Studies of the mechanism of the .DELTA.5-3-ketosteroid isomerase reaction by substrate, solvent, and combined kinetic deuterium isotope effects on wild-type and mutant enzymes

1990 • 57 citations

Glutathione reductase: comparison of steady-state and rapid reaction primary kinetic isotope effects exhibited by the yeast, spinach, and Escherichia coli enzymes

1990 • 54 citations

Positioning of a spin-labeled substrate analog into the structure of .DELTA.5-3-ketosteroid isomerase by combined kinetic, magnetic resonance, and x-ray diffraction methods

1987 • 45 citations

Amino acid activation in crystalline tyrosyl-tRNA synthetase from Bacillus stearothermophilus

1981 • 44 citations

Diverse interactions between the individual mutations in a double mutant at the active site of staphylococcal nuclease

1990 • 44 citations

Stereochemistry of the concerted enolization catalyzed by .DELTA.5-3-ketosteroid isomerase

1991 • 42 citations

Catalytic mechanism of an active-site mutant (D38N) of .DELTA.5-3-ketosteroid isomerase

1991 • 40 citations

Active site mutant Glu-43 .fwdarw. Asp in staphylococcal nuclease displays nonlocal structural changes

1990 • 38 citations

Studies of the catalytic mechanism of an active-site mutant (Y14F) of .DELTA.5-3-ketosteroid isomerase by kinetic deuterium isotope effects

1991 • 37 citations

Conformation of an enzyme-bound substrate of staphylococcal nuclease as determined by NMR

1991 • 35 citations

Kinetic and magnetic resonance studies of the glutamate-43 to serine mutant of staphylococcal nuclease

1989 • 31 citations

Structure of a mutant of tyrosyl-tRNA synthetase with enhanced catalytic properties

1987 • 29 citations

Direct determination of the partitioning of an enzyme-bound intermediate

1990 • 24 citations