Catalytic mechanism of an active-site mutant (D38N) of .DELTA.5-3-ketosteroid isomerase

A Schematic Method of Deriving the Rate Laws for Enzyme-Catalyzed Reactions

1956 • 994 citations

Stereoelectronic Control in Enolization-Ketonization Reactions¹

1956 • 209 citations

Reaction-coordinate tunneling in hydride-transfer reactions

1983 • 133 citations

Chemistry of Conjugate Anions and Enols. V. Stereochemistry, Kinetics, and Mechanism of the Acid- and Enzymatic-Catalyzed Isomerization of ⁵-3-Keto Steroids^1,2

1965 • 123 citations

Kinetic and ultraviolet spectroscopic studies of active-site mutants of .DELTA.5-3-ketosteroid isomerase

1989 • 122 citations

The Mechanism of the Δ5-3-Ketosteroid Isomerase Reaction: Absorption and Fluorescence Spectra of Enzyme-Steroid Complexes

1963 • 111 citations

Primary and secondary deuterium isotope effects on equilibrium constants for enzyme-catalyzed reactions

1980 • 107 citations

Enzyme-catalyzed allylic rearrangements

1990 • 89 citations

The Δ5-3-ketosteroid isomerase reaction: catalytic mechanism, specificity and inhibition

1976 • 72 citations

MULTIPLE ISOTOPE EFFECTS ON ENZYME-CATALYZED REACTIONS

1989 • 65 citations

Isolation and sequencing of the gene encoding delta 5-3-ketosteroid isomerase of Pseudomonas testosteroni: overexpression of the protein.

1987 • 60 citations

Combined effects of two mutations of catalytic residues on the ketosteroid isomerase reaction

1990 • 59 citations

Anomalous equilibrium and kinetic .alpha.-deuterium secondary isotope effects accompanying hydride transfer from reduced nicotinamide adenine dinucleotide

1980 • 59 citations

Studies of the mechanism of the .DELTA.5-3-ketosteroid isomerase reaction by substrate, solvent, and combined kinetic deuterium isotope effects on wild-type and mutant enzymes

1990 • 57 citations

pH dependence of the kinetic parameters for 3-oxo-.DELTA.5-steroid isomerase. Substrate catalysis and inhibition by (3S)-spiro[5.alpha.-androstane-3,2'-oxiran]-17-one

1986 • 50 citations

Concentration-dependent association of delta5-3-ketosteroid isomerase of Pseudomonas testosteroni.

1975 • 47 citations

Positioning of a spin-labeled substrate analog into the structure of .DELTA.5-3-ketosteroid isomerase by combined kinetic, magnetic resonance, and x-ray diffraction methods

1987 • 45 citations

Stereochemistry of the concerted enolization catalyzed by .DELTA.5-3-ketosteroid isomerase

1991 • 42 citations

Direct determination of the partitioning of an enzyme-bound intermediate

1990 • 24 citations

Cloning of the gene for Δ5-3-ketosteroid isomerase from Pseudomonas testosteroni

1987 • 16 citations

Evidence for an enol intermediate in the 3-oxo-.DELTA.5-steroid isomerase catalyzed isomerization of .DELTA.5 ketones

1986 • 14 citations

Synthese des 4α et 4β-deuterio 3β, 17β-dihydroxy androsta-5-ene

1978 • 9 citations