Natural variation of tyrosyl-tRNA synthetase and comparison with engineered mutants

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

A procedure for the isolation of deoxyribonucleic acid from micro-organisms

1961 • 11,554 citations

The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers

1982 • 6,153 citations

A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments

1982 • 3,041 citations

Hydrogen bonding and biological specificity analysed by protein engineering

1985 • 1,050 citations

A reliable method for the recovery of DNA fragments from agarose and acrylamide gels

1981 • 916 citations

A mew computer method for the storage and manipulation of DNA gel reading data

1980 • 679 citations

The use of double mutants to detect structural changes in the active site of the tyrosyl-tRNA synthetase (Bacillus stearothermophilus)

1984 • 563 citations

Redesigning enzyme structure by site-directed mutagenesis: tyrosyl tRNA synthetase and ATP binding

1982 • 348 citations

Site-directed mutagenesis as a probe of enzyme structure and catalysis: tyrosyl-tRNA synthetase cysteine-35 to glycine-35 mutation

1983 • 268 citations

Specific Sequence Homology and Three-Dimensional Structure of an Aminoacyl Transfer RNA Synthetase

1984 • 264 citations

Transition-state stabilization in the mechanism of tyrosyl-tRNA synthetase revealed by protein engineering.

1985 • 179 citations

Tyrosyl-tRNA synthetase forms a mononucleotide-binding fold

1982 • 149 citations

A large increase in enzyme–substrate affinity by protein engineering

1984 • 147 citations

Crystal structure of Escherichia coli methionyl-tRNA synthetase at 2.5 Å resolution

1982 • 136 citations

The Amino Acid Sequence of the Tyrosy1-tRNA Synthetase from Bacillus stearothermophilus

1983 • 112 citations

A computer program to search for tRNA genes.

1980 • 111 citations

Characteristics and properties of a caldo-active bacterium producing extracellular enzymes and two related strains

1972 • 103 citations

Escherichia coli glutaminyl-tRNA synthetase. II. Characterization of the glnS gene product.

1982 • 97 citations

Analysis of Enzyme Structure and Activity by Protein Engineering

1984 • 97 citations

Tyrosyl-tRNA synthetase from Escherichia coli. Stoichiometry of ligand binding and half-of-the-sites reactivity in aminoacylation

1975 • 91 citations

Methionyl-tRNA synthetase from Escherichia coli. Primary structure of the active crystallised tryptic fragment.

1982 • 89 citations

Probing histidine-substrate interactions in tyrosyl-tRNA synthetase using asparagine and glutamine replacements

1985 • 88 citations

Primary structure of the Saccharomyces cerevisiae gene for methionyl-tRNA synthetase.

1983 • 77 citations

The tyrosyl‐tRNA synthetase from Escherichia coli

1982 • 69 citations

The nucleotide sequence of the structural gene for Escherichia coli tryptophanyl-tRNA synthetase.

1982 • 68 citations

Structural homology in the amino-terminal domains of two aminoacyl-tRNA synthetases

1983 • 68 citations

The amino acid sequence of tryptophanyl tRNA Synthetase from Bacillus stearothermophilus

1977 • 52 citations

Cloning and Amplified Expression of the Tyrosyl-tRNA Synthetase Genes of Bacillus stearothermophilus and Escherichia coli

1982 • 46 citations

Amino acid activation in crystalline tyrosyl-tRNA synthetase from Bacillus stearothermophilus

1981 • 44 citations

Fine structure-activity analysis of mutations at position 51 of tyrosyl-tRNA synthetase

1985 • 31 citations