Kinetic and ultraviolet spectroscopic studies of active-site mutants of .DELTA.5-3-ketosteroid isomerase

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Rapid and efficient site-specific mutagenesis without phenotypic selection.

1985 • 7,573 citations

The Conservation of Orbital Symmetry

1969 • 3,870 citations

Statistical estimations in enzyme kinetics

1961 • 3,586 citations

Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis

1983 • 2,609 citations

The spectrophotometric determination of tyrosine and tryptophan in proteins

1946 • 1,515 citations

[32] Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors

1983 • 1,093 citations

Analytical Gel Chromatography of Proteins

1970 • 369 citations

Ultraviolet Absorption of Steroids.

1953 • 313 citations

The Catalytic Role of the Active Site Aspartic Acid in Serine Proteases

1987 • 296 citations

The Three-Dimensional Structure of Asn ¹⁰² Mutant of Trypsin: Role of Asp ¹⁰² in Serine Protease Catalysis

1987 • 212 citations

Interpretations of enzyme reaction stereospecificity

1975 • 160 citations

Enzymic isomerization of Δ5-3-ketosteroids

1955 • 139 citations

Chemistry of Conjugate Anions and Enols. V. Stereochemistry, Kinetics, and Mechanism of the Acid- and Enzymatic-Catalyzed Isomerization of ⁵-3-Keto Steroids^1,2

1965 • 123 citations

3-Carbanionic substrate analogues bind very tightly to fumarase and aspartase.

1980 • 114 citations

The Mechanism of the Δ5-3-Ketosteroid Isomerase Reaction: Absorption and Fluorescence Spectra of Enzyme-Steroid Complexes

1963 • 111 citations

The Preparation and Properties of Crystalline Δ5-3-Ketosteroid Isomerase

1962 • 107 citations

Use of isotope effects to deduce the chemical mechanism of fumarase

1980 • 85 citations

Isotopic Probes of the Enolase Reaction Mechanism

1971 • 72 citations

Nitro analogs of citrate and isocitrate as transition-state analogs for aconitase

1980 • 72 citations

The Δ5-3-ketosteroid isomerase reaction: catalytic mechanism, specificity and inhibition

1976 • 72 citations

Isolation and sequencing of the gene encoding delta 5-3-ketosteroid isomerase of Pseudomonas testosteroni: overexpression of the protein.

1987 • 60 citations

Affinity labeling of the active sites of Δ⁵ ketosteroid isomerase using photoexcited natural ligands

1973 • 57 citations

pH dependence of the kinetic parameters for 3-oxo-.DELTA.5-steroid isomerase. Substrate catalysis and inhibition by (3S)-spiro[5.alpha.-androstane-3,2'-oxiran]-17-one

1986 • 50 citations

Concentration-dependent association of delta5-3-ketosteroid isomerase of Pseudomonas testosteroni.

1975 • 47 citations

A novel chemical modification of delta 5-3-ketosteroid isomerase occurring during its 3-oxo-4-estren-17 beta-yl acetate-dependent photoinactivation.

1977 • 46 citations

Further Studies of Steroidal Inhibitors of Δ⁵,3β-Hydroxysteroid Dehydrogenase and Δ⁵-Δ⁴,3-Ketosteroid Isomerase inPseudomonas testosteroniand in Bovine Adrenals

1968 • 46 citations

Positioning of a spin-labeled substrate analog into the structure of .DELTA.5-3-ketosteroid isomerase by combined kinetic, magnetic resonance, and x-ray diffraction methods

1987 • 45 citations

The 6-A crystal structure of delta 5-3-ketosteroid isomerase. Architecture and location of the active center.

1984 • 42 citations

Affinity alkylation of 3-oxo-.DELTA.5-steroid isomerase by steroidal 3.beta.-oxiranes: identification of the modified amino acid by reduction with hydroxyborohydride

1987 • 31 citations

Chemical Modification of Amino Acid Residues Associated with the δ4-3-Ketosteroid-dependent Photoinactivation of δ5-3Xetosteroid Isomerase

1975 • 31 citations

Etude de la Delta54 3-oxosteroide isomerase. Caracteristiques des groupes impliques dans le transfert de protons

1970 • 29 citations

An active-site-directed irreversible inhibitor of Δ5-3-ketosteroid isomerase

1979 • 27 citations

Modification of an enzyme carboxylate residue in the inhibition of 3-oxo-.DELTA.5-steroid isomerase by (3S)-spiro[5.alpha.-androstane-3,2'-oxirane]-17.beta.-ol. Implications for the mechanism of action

1984 • 26 citations

Irreversible active-site-directed inhibition of Δ5-3-ketosteroid isomerase by steroidal 17-β-oxiranes. Evidence for two modes of binding in steroid-enzyme complexes

1980 • 24 citations

Recent Studies on Steroid Isomerases From Pseudomonas Testosteroni and Pseudomonas Putida *

1980 • 22 citations

Affinity alkylation of bacterial delta 5-3-ketosteroid isomerase. Identification of the amino acid modified by steroidal 17 beta-oxiranes.

1983 • 22 citations

Molecular structures of substrates and inhibitors of .DELTA.5-3-keto steroid isomerase and their relevance to the enzymic mechanism

1978 • 21 citations

Enzymatic function in crystals of delta 5-3-ketosteroid isomerase. Catalytic activity and binding of competitive inhibitors.

1984 • 20 citations

Studies on Δ5→4-3-oxo steroid isomerases. I. Extraction model for enzymic activity

1968 • 20 citations

Use of a solid-phase photoaffinity reagent to label a steroid binding site: application to the .DELTA.5-3-ketosteroid isomerase of Pseudomonas testosteroni

1985 • 19 citations

Cloning of the gene for Δ5-3-ketosteroid isomerase from Pseudomonas testosteroni

1987 • 16 citations

Proton nuclear magnetic resonance studies of Pseudomonas testosteroni 3-oxo-.DELTA.5-steroid isomerase and its interaction with 17.beta.-estradiol

1982 • 15 citations

Detection of a transient enzyme-steroid complex during active-site-directed irreversible inhibition of 3-oxo-.DELTA.5-steroid isomerase

1986 • 13 citations

Influence of the position of the double bond in steroid substrates on the efficiency of the proton-transfer reaction by Pseudomonas testosteroni 3-oxo steroid Δ4–Δ5-isomerase

1980 • 10 citations