Site-directed mutagenesis shows that tyrosine 248 of carboxypeptidase A does not play a crucial role in catalysis

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A

1981 • 8,657 citations

Transformation of intact yeast cells treated with alkali cations

1983 • 7,688 citations

A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments

1982 • 3,041 citations

Hydrogen bonding and biological specificity analysed by protein engineering

1985 • 1,050 citations

Oligonucleotide-Directed Mutagenesis: A Simple Method Using Two Oligonucleotide Primers and a Single-Stranded DNA Template

1984 • 947 citations

Isolation of the putative structural gene for the lysine-arginine-cleaving endopeptidase required for processing of yeast prepro-α-factor

1984 • 698 citations

Structure of a yeast pheromone gene (MFα): A putative α-factor precursor contains four tandem copies of mature α-factor

1982 • 587 citations

Mutagenesis at a specific position in a DNA sequence.

1978 • 504 citations

Alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae.

1984 • 477 citations

Glycosylation and processing of prepro-α-factor through the yeast secretory pathway

1984 • 410 citations

Refined crystal structure of carboxypeptidase a at 1·54 Å resolution

1983 • 391 citations

Redesigning Trypsin: Alteration of Substrate Specificity

1985 • 357 citations

Functional Tyrosyl Residues in the Active Center of Bovine Pancreatic Carboxypeptidase A^*

1963 • 302 citations

The graphical determination of Km and Ki

1972 • 290 citations

Refined crystal structure of the potato inhibitor complex of carboxypeptidase A at 2.5 Å resolution

1982 • 260 citations

An MF alpha 1-SUC2 (alpha-factor-invertase) gene fusion for study of protein localization and gene expression in yeast.

1983 • 187 citations

[21] Construction of high copy yeast vectors using 2-μm circle sequences

1983 • 158 citations

Binding of N-carboxymethyl dipeptide inhibitors to thermolysin determined by x-ray crystallography: a novel class of transition-state analogs for zinc peptidases

1984 • 153 citations

THE AMINO ACID SEQUENCE OF BOVINE CARBOXYPEPTIDASE A

1969 • 140 citations

Secretion of foreign proteins from Saccharomyces cerevisiae directed by alpha-factor gene fusions.

1984 • 126 citations

Chemical synthesis of a gene for human epidermal growth factor urogastrone and its expression in yeast.

1983 • 115 citations

Kinetics of carboxypeptidase A. I. Hydrolysis of carbobenzoxyglycyl-L-phenylalanine, benzoylglycyl-L-phenylalanine, and hippuryl-DL-.beta.-phenyllactic acid by metal-substituted and acetylated carboxypeptidases

1968 • 113 citations

Kinetics of carboxypeptidase A. II. Inhibitors of the hydrolysis of oligopeptides

1970 • 103 citations

The Kinetics of Some Carboxypeptidase A and Acetylcarboxypeptidase A Catalyzed Hydrolyses^*

1966 • 94 citations

Rat preprocarboxypeptidase A: cDNA sequence and preliminary characterization of the gene.

1982 • 91 citations

The Functional Tyrosyl Residues of Carboxypeptidase A. Nitration with Tetranitromethane^*

1967 • 86 citations

Carboxypeptidase A mechanisms.

1980 • 76 citations

Carboxypeptidase A. Mechanistic analysis

1972 • 71 citations

Binding of ligands to the active site of carboxypeptidase A.

1981 • 57 citations

The pH dependence of the nitrotyrosine-248 and arsanilazotyrosine-248 carboxypeptidase A catalyzed hydrolysis of O-(trans-p-chlorocinnamoyl)-L-.beta.-phenyllactate

1976 • 50 citations

Nature of the bonding between silicon and the cobalt tetracarbonyl group in silyl cobalt tetracarbonyls

1970 • 43 citations

Species Variation of the RNA Methylases^*

1964 • 34 citations

Affinity chromatographic sorting of carboxypeptidase a and its chemically modified derivatives

1980 • 31 citations

Intramolecular arsanilazotyrosine-248-Zn complex of carboxypeptidase A: a monitor of multiple conformational states in solution.

1975 • 23 citations

Chemical modification of carboxypeptidase A crystals. Nitration of tyrosine-248

1976 • 18 citations

A peptidase-inactive derivative of carboxypeptidase A modified specifically at tyrosine 248. Cobalt(III) (ethylenediamine-N,N'-diacetato) (arsanilazotyrosinato 248 carboxypeptidase A).

1979 • 15 citations

The pH dependence of peptide hydrolysis by nitrocarboxypeptidase A

1980 • 15 citations

On the action of carboxypeptidase a on ester substrates in alkaline solution

1972 • 7 citations