Processing of Mouse Proglucagon by Recombinant Prohormone Convertase 1 and Immunopurified Prohormone Convertase 2 in Vitro

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

Molecular cloning: A laboratory manual

1990 • 85,659 citations

Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

1987 • 64,270 citations

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

1987 • 45,673 citations

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

1988 • 6,107 citations

PERIODATE-LYSINE-PARAFORMALDEHYDE FIXATIVE A NEW FIXATIVE FOR IMMUNOELECTRON MICROSCOPY

1974 • 3,882 citations

Isolation of the putative structural gene for the lysine-arginine-cleaving endopeptidase required for processing of yeast prepro-α-factor

1984 • 698 citations

Preproglucagon gene expression in pancreas and intestine diversifies at the level of post-translational processing.

1986 • 686 citations

Post-Translational Proteolysis in Polypeptide Hormone Biosynthesis

1982 • 590 citations

PC1 and PC2 are proprotein convertases capable of cleaving proopiomelanocortin at distinct pairs of basic residues.

1991 • 589 citations

Identification of a human insulinoma cDNA encoding a novel mammalian protein structurally related to the yeast dibasic processing protease Kex2.

1990 • 579 citations

Cloning and Primary Sequence of a Mouse Candidate Prohormone Convertase PC1 Homologous to PC2, Furin, and Kex2: Distinct Chromosomal Localization and Messenger RNA Distribution in Brain and Pituitary Compared to PC2

1991 • 475 citations

Identification of a cDNA encoding a second putative prohormone convertase related to PC2 in AtT20 cells and islets of Langerhans.

1991 • 449 citations

Enzymes Required for Yeast Prohormone Processing

1988 • 446 citations

Intraorganellar calcium and pH control proinsulin cleavage in the pancreatic β cell via two distinct site-specific endopeptidases

1988 • 403 citations

Intracellular Targeting and Structural Conservation of a Prohormone-Processing Endoprotease

1989 • 395 citations

Human fur gene encodes a yeast KEX2-like endoprotease that cleaves pro-beta-NGF in vivo.

1990 • 378 citations

Proinsulin processing by the subtilisin-related proprotein convertases furin, PC2, and PC3.

1992 • 300 citations

Expression of a human proprotein processing enzyme: correct cleavage of the von Willebrand factor precursor at a paired basic amino acid site.

1990 • 296 citations

Glucagon-like peptide-1, a new hormone of the entero-insular axis

1992 • 290 citations

Identification of a Second Human Subtilisin-Like Protease Gene in thefes/fpsRegion of Chromosome 15

1991 • 268 citations

Furin is a subtilisin-like proprotein processing enzyme in higher eukaryotes

1990 • 259 citations

Distribution and regulation of the prohormone convertases PC1 and PC2 in the rat pituitary.

1992 • 256 citations

Identification of the fourth member of the mammalian endoprotease family homologous to the yeast Kex2 protease. Its testis-specific expression.

1992 • 249 citations

Prohormone-Converting Enzymes: Regulation and Evaluation of Function Using Antisense RNA

1991 • 229 citations

cDNA structure of the mouse and rat subtilisin/kexin-like PC5: a candidate proprotein convertase expressed in endocrine and nonendocrine cells.

1993 • 223 citations

Structure and expression of mouse furin, a yeast Kex2-related protease. Lack of processing of coexpressed prorenin in GH4C1 cells.

1990 • 219 citations

Proprotein-processing endoproteases PC6 and furin both activate hemagglutinin of virulent avian influenza viruses

1994 • 215 citations

Proglucagon is processed to glucagon by prohormone convertase PC2 in alpha TC1-6 cells.

1994 • 211 citations

Testicular expression of PC4 in the rat: molecular diversity of a novel germ cell-specific Kex2/subtilisin-like proprotein convertase.

1992 • 203 citations

Structural homology between the human fur gene product and the subtilisin-like protease encoded by yeast KEX2

1990 • 202 citations

Identification and Functional Expression of a New Member of the Mammalian Kex2-Like Processing Endoprotease Family: Its Striking Structural Similarity to PACE41

1993 • 185 citations

Proglucagon processing in porcine and human pancreas.

1994 • 167 citations

Cellular localization of proglucagon/glucagon‐like peptide I messenger RNAs in rat brain

1986 • 165 citations

Purification and characterization of the prohormone convertase PC1(PC3).

1993 • 165 citations

Differential processing of proenkephalin by prohormone convertases 1(3) and 2 and furin.

1993 • 151 citations

Identification of the type 2 proinsulin processing endopeptidase as PC2, a member of the eukaryote subtilisin family.

1992 • 148 citations

The neuroendocrine polypeptide 7B2 is an endogenous inhibitor of prohormone convertase PC2.

1994 • 141 citations

Proglucagon Processing Similar to Normal Islets in Pancreatic α-Like Cell Line Derived From Transgenic Mouse Tumor

1990 • 134 citations

Ontogeny of the prohormone convertases PC1 and PC2 in the mouse hypophysis and their colocalization with corticotropin and alpha-melanotropin.

1993 • 129 citations

Cloning and Functional Expression of a Novel Endoprotease Involved in Prohormone Processing at Dibasic Sites1

1991 • 96 citations

Developmental expression of the prohormone convertases PC1 and PC2 in mouse pancreatic islets.

1994 • 87 citations

Isolation of Two Complementary Deoxyribonucleic Acid Clones from a Rat Insulinoma Cell Line Based on Similarities to Kex2 and Furin Sequences and the Specific Localization of Each Transcript to Endocrine and Neuroendocrine Tissues in Rats

1991 • 79 citations

Biosynthesis of the prohormone convertase PC2 in Chinese hamster ovary cells and in rat insulinoma cells.

1993 • 77 citations

pH-independent and -dependent cleavage of proinsulin in the same secretory vesicle.

1994 • 73 citations

Isolation and functional expression of a mammalian prohormone processing enzyme, murine prohormone convertase 1.

1991 • 57 citations

Levels of the conversion endoproteases PC1 (PC3) and PC2 distinguish between insulin-producing pancreatic islet β cells and non-β cells

1994 • 52 citations

Precursors to regulatory peptides: their proteolytic processing

1987 • 46 citations

Glucagon and Related Peptides in Fetal Rat Hypothalamusin Vivoandin Vitro*

1990 • 43 citations

Biogenesis of storage granules and vesicles

1992 • 38 citations

Direct evidence for two distinct prosomatostatin converting enzymes. Detection using a rapid, sensitive, and specific assay for propeptide converting enzymes.

1987 • 36 citations

Distribution of the Kexin family proteases in pancreatic islets: PACE4C is specifically expressed in B cells of pancreatic islets.

1995 • 31 citations

Identification of Novel cDNAs Encoding Human Kexin-Like Protease, PACE4 Isoforms

1994 • 27 citations

A Novel Member, PC7, of the Mammalian Kexin-like Protease Family - Homology to PACE4A, Its Brain-Specific Expression and Identification of Isoforms

1994 • 24 citations

Specific glucagon-related peptides isolated from anglerfish islets are metabolic cleavage products of (pre)proglucagon-II

1986 • 22 citations

Immunological detection of prohormone convertases in two different proglucagon processing cell lines

1994 • 19 citations

Fluorometric assay of a calcium-dependent, paired-basic processing endopeptidase present in insulinoma granules

1992 • 19 citations

Characterization of aPY-like peptides in anglerfish brain using a novel radioimmunoassay for aPY-Gly

1989 • 11 citations

Proglucagon-Derived Peptides in the Neuroendocrine System

1991 • 1 citations