The use of a selectable FokI cassette in dna replacement mutagenesis of the R388 dihydrofolate reductase gene

The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers

1982 • 6,153 citations

Supercoil Sequencing: A Fast and Simple Method for Sequencing Plasmid DNA

1985 • 2,505 citations

The isolation of structural genes from libraries of eucaryotic DNA

1978 • 1,877 citations

In vitro insertional mutagenesis with a selectable DNA fragment

1984 • 1,708 citations

Transcriptional Control Signals of a Eukaryotic Protein-Coding Gene

1982 • 1,406 citations

[32] Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors

1983 • 1,093 citations

Oligonucleotide synthesis : a practical approach

1984 • 491 citations

Alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae.

1984 • 477 citations

The use of transposon Tn5 mutagenesis in the rapid generation of correlated physical and genetic maps of DNA segments cloned into multicopy plasmids — a review

1984 • 422 citations

IN VITRO MUTAGENESIS

1985 • 338 citations

Cassette mutagenesis: an efficient method for generation of multiple mutations at defined sites

1985 • 270 citations

Purification of genomic sequences from bacteriophage libraries by recombination and selectionin vivo

1983 • 221 citations

In vitro mutagenesis of a circular DNA molecule by using synthetic restriction sites.

1978 • 200 citations

Strategies and Applications of in Vitro Mutagenesis

1985 • 192 citations

Mapping of closed circular DNAs by cleavage with restriction endonucleases and calibration by agarose gel electrophoresis.

1977 • 179 citations

Specific amino acid substitutions in bacterioopsin: Replacement of a restriction fragment in the structural gene by synthetic DNA fragments containing altered codons

1984 • 158 citations

Single-stranded hexameric linkers: a system for in-phase insertion mutagenesis and protein engineering

1985 • 151 citations

Mutagenesis of the three bases preceding the start codon of the beta-galactosidase mRNA and its effect on translation in Escherichia coli.

1984 • 128 citations

Tn1721 derivatives for transposon mutagenesis, restriction mapping and nucleotide sequence analysis

1986 • 120 citations

Chemical synthesis of a gene for human epidermal growth factor urogastrone and its expression in yeast.

1983 • 115 citations

Identification of functional regions in the transforming protein of Fujinami sarcoma virus by in-phase insertion mutagenesis

1984 • 113 citations

Oligonucleotide-directed mutagenesis by microscale ‘shot-gun’ gene synthesis

1985 • 105 citations

Universal restriction endonucleases: designing novel cleavage specificities by combining adapter oligodeoxynucleotide and enzyme moieties

1985 • 91 citations

Oligonucleotide synthesis. a practical approach

1985 • 87 citations

DNA sequence of a plasmid-encoded dihydrofolate reductase

1981 • 82 citations

Conversion of the FokI endonuclease to a universal restriction enzyme: cleavage of phage M13mp7 DNA at predetermined sites

1985 • 80 citations

Site-saturation studies of beta-lactamase: production and characterization of mutant beta-lactamases with all possible amino acid substitutions at residue 71.

1986 • 73 citations

Ω mutagenesis in gram-negative bacteria: a selectable interposon which is strongly polar in a wide range of bacterial species

1985 • 68 citations

Nucleotide sequence of the xanthine guanine phosphoribosyl transferase gene ofE. coli

1983 • 62 citations

Chemical synthesis of a gene for somatomedin C

1985 • 61 citations

Construction of mutants of Moloney murine leukemia virus by suppressor-linker insertional mutagenesis: positions of viable insertion mutations.

1984 • 61 citations

Expression inEscherichiaof chemically synthesized gene for the human immune Interferon

1983 • 56 citations

Chemical synthesis of a human interferon-α², gene and its expression inEscherichia coli

1983 • 44 citations

A rapid procedure for DNA sequencing using transposon-promoted deletions in Escherichia coli

1985 • 43 citations

Chemical synthesis of a biologically active gene for human immune interferon-gamma. Prospect for site-specific mutagenesis and structure-function studies.

1984 • 37 citations

Altered Cro repressors from engineered mutagenesis of a synthetic cro gene.

1985 • 29 citations

Use of bacterial DHFR-II fusion proteins to elicit specific antibodies

1986 • 19 citations

Cloning of small DNA fragments containing the Escherichia coli tryptophan operon promoter and operator

1982 • 19 citations

Mutational analysis of the cloned chicken thymidine kinase gene.

1983 • 15 citations

Construction and characterization of pBR322-derived plasmids with deletions of the RNA I region

1986 • 14 citations

The MURFI linker for multiple reading frame insertion of a sense or nonsense codon into DNA

1986 • 14 citations

Tn951 derivatives designed for high-frequency plasmid-specific transposition and deletion mutagenesis

1986 • 12 citations

Specific recombination in vitro promoted by the restriction endonuclease HgaI

1979 • 12 citations

Formation of MboII vectors and cassettes using asymmetric MboII linkers

1987 • 11 citations

Site-specific DNA splicing: a general procedure for the creation of a restriction site at a pre-determined position in a DNA sequence

1986 • 5 citations

Deleted Work

1955 • 0 citations