Cassette mutagenesis: an efficient method for generation of multiple mutations at defined sites

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

Experiments in molecular genetics

1972 • 28,260 citations

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

1979 • 15,511 citations

Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing

1980 • 3,476 citations

A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments

1982 • 3,041 citations

Calcium-dependent bacteriophage DNA infection

1970 • 2,743 citations

A system for shotgun DNA sequencing

1981 • 2,710 citations

Supercoil Sequencing: A Fast and Simple Method for Sequencing Plasmid DNA

1985 • 2,505 citations

Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA

1982 • 764 citations

Structure of Subtilisin BPN′ at 2.5 Å Resolution

1969 • 543 citations

Mutagenesis at a specific position in a DNA sequence.

1978 • 504 citations

Cloning, sequencing, and secretion of Bacillusamyloliquefacienssubtillisin inBacillus subtilis

1983 • 357 citations

Redesigning enzyme structure by site-directed mutagenesis: tyrosyl tRNA synthetase and ATP binding

1982 • 348 citations

Cloning of the neutral protease gene of Bacillus subtilis and the use of the cloned gene to create an in vitro-derived deletion mutation

1984 • 257 citations

Oligonucleotide-directed mutagenesis as a general and powerful method for studies of protein function.

1982 • 211 citations

Directed Mutagenesis of Dihydrofolate Reductase

1983 • 210 citations

Oligonucleotide directed mutagenesis of the human β-globin gene: a general method for producing specific point mutations in cloned DNA

1981 • 207 citations

Bacillus subtilis Requires a "Stringent" Shine-Dalgarno Region for Gene Expression

1984 • 155 citations

Efficient site-directed mutagenesis by simultaneous use of two primers

1983 • 154 citations

Mutagenesis of the three bases preceding the start codon of the beta-galactosidase mRNA and its effect on translation in Escherichia coli.

1984 • 128 citations

Synthesis of oligonucleotides on cellulose by a phosphotriester method

1980 • 113 citations

Efficient correction of a mutation by use of chemically synthesized DNA.

1978 • 112 citations

Targeted random mutagenesis: the use of ambiguously synthesized oligonucleotides to mutagenize sequences immediately 5‘ of an ATG initiation codon

1983 • 112 citations

The Effect on Subtilisin Activity of Oxidizing a Methionine Residue

1969 • 107 citations

Subtilisin Novo. The Three-Dimensional Structure and Its Comparison with Subtilisin BPN'

1972 • 104 citations

In vitrosite-directed mutagenesis with synthetic DNA oligonucleotides yields unexpected deletions and insertions at high frequency

1983 • 33 citations