The role of small nuclear ribonucleoprotein particles in pre-mRNA splicing

Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter

1984 • 6,459 citations

Transcription termination and 3′ processing: the end is in site!

1985 • 1,482 citations

SPLICING OF MESSENGER RNA PRECURSORS

1986 • 1,233 citations

Are snRNPs involved in splicing?

1980 • 1,206 citations

Antibodies to small nuclear RNAs complexed with proteins are produced by patients with systemic lupus erythematosus

1979 • 1,178 citations

Excision of an intact intron as a novel lariat structure during pre-mRNA splicing in vitro

1984 • 761 citations

A compensatory base change in U1 snRNA suppresses a 5′ splice site mutation

1986 • 755 citations

Macromolecule Synthesis in Temperature-sensitive Mutants of Yeast

1967 • 728 citations

Normal and mutant human β-globin pre-mRNAs are faithfully and efficiently spliced in vitro

1984 • 679 citations

Specific transcription and RNA splicing defects in five cloned β-thalassaemia genes

1983 • 627 citations

The U1 small nuclear RNA-protein complex selectively binds a 5′ splice site in vitro

1983 • 593 citations

U2 as well as U1 small nuclear ribonucleoproteins are involved in premessenger RNA splicing

1985 • 568 citations

A mechanism for RNA splicing.

1980 • 553 citations

Stepwise assembly of a pre-mRNA splicing complex requires U-snRNPs and specific intron sequences

1985 • 524 citations

Human β-globin pre-mRNA synthesized in vitro is accurately spliced in xenopus oocyte nuclei

1983 • 500 citations

Biological Catalysis by RNA

1986 • 496 citations

A role for exon sequences and splice-site proximity in splice-site selection

1986 • 494 citations

Electrophoretic separation of complexes involved in the splicing of precursors to mRNAs

1986 • 490 citations

Lariat RNA's as Intermediates and Products in the Splicing of Messenger RNA precursors

1984 • 474 citations

A multicomponent complex is involved in the splicing of messenger RNA precursors

1985 • 451 citations

A self-splicing RNA excises an intron lariat

1986 • 449 citations

Secondary Structure of 16 S Ribosomal RNA

1981 • 446 citations

Multiple factors including the small nuclear ribonucleoproteins U1 and U2 are necessary for Pre-mRNA splicing in vitro

1985 • 442 citations

The "Spliceosome": Yeast Pre-Messenger RNA Associates with a 40 S Complex in a Splicing-Dependent Reaction

1985 • 421 citations

The 5′ terminus of the RNA moiety of U1 small nuclear ribonucleoprotein particles is required for the splicing of messenger RNA precursors

1984 • 404 citations

Intron sequences involved in lariat formation during pre-mRNA splicing

1985 • 402 citations

Heterogeneous Nuclear Ribonucleoproteins: Role in RNA Splicing

1986 • 399 citations

Evidence for an intron-contained sequence required for the splicing of yeast RNA polymerase II transcripts

1983 • 396 citations

Lariat structures are in vivo intermediates in yeast pre-mRNA splicing

1984 • 379 citations

Excised group II introns in yeast mitochondria are lariats and can be formed by self-splicing in vitro

1986 • 346 citations

Intron splicing: a conserved internal signal in introns of animal pre-mRNAs.

1984 • 342 citations

Point mutations identify the conserved, intron-contained TACTAAC box as an essential splicing signal sequence in yeast

1984 • 339 citations

Yeast mRNA splicing in vitro.

1985 • 337 citations

Accurate cleavage and polyadenylation of exogenous RNA substrate

1985 • 334 citations

Splicing of messenger RNA precursors is inhibited by antisera to small nuclear ribonucleoprotein

1983 • 333 citations

The 3′ Splice Site of Pre-Messenger RNA Is Recognized by a Small Nuclear Ribonucleoprotein

1985 • 310 citations

A protein associated with small nuclear ribonucleoprotein particles recognizes the 3′ splice site of premessenger RNA

1986 • 283 citations

Electrophoresis of ribonucleoproteins reveals an ordered assembly pathway of yeast splicing complexes

1986 • 283 citations

A protein that specifically recognizes the 3′ splice site of mammalian pre-mRNA introns is associated with a small nuclear ribonucleoprotein

1986 • 274 citations

Messenger RNA splicing in vitro: An excised intervening sequence and a potential intermediate

1984 • 262 citations

Splicing of in vitro synthesized messenger RNA precursors in HeLa cell extracts

1983 • 255 citations

A small nuclear ribonucleoprotein is required for splicing of adenoviral early RNA sequences.

1981 • 243 citations

Evidence for the biochemical role of an internal sequence in yeast nuclear mRNA introns: Implications for U1 RNA and metazoan mRNA splicing

1983 • 239 citations

Affinity Chromatography of Splicing Complexes: U2, U5, and U4 + U6 Small Nuclear Ribonucleoprotein Particles in the Spliceosome

1986 • 238 citations

U4 and U6 RNAs coexist in a single small nuclear ribonucleoprotein particle

1984 • 238 citations

Cryptic branch point activation allows accurate in vitro splicing of human β-globin intron mutants

1985 • 236 citations

Unusual splice sites revealed by mutagenic inactivation of an authentic splice site of the rabbit β-globin gene

1983 • 235 citations

Pre-mRNA splicing in vitro requires intact U4/U6 small nuclear ribonucleoprotein

1986 • 222 citations

Mutations in conserved intron sequences affect multiple steps in the yeast splicing pathway, particularly assembly of the spliceosome.

1986 • 215 citations

Evidence for the existence of snRNAs U4 and U6 in a single ribonucleoprotein complex and for their association by intermolecular base pairing.

1984 • 213 citations

In vivo splicing products of the rabbit β-globin pre-mRNA

1984 • 209 citations

U1, U2, and U4/U6 small nuclear ribonucleoproteins are required for in vitro splicing but not polyadenylation

1986 • 192 citations

Schizosaccharomyces pombe and saccharomyces cerevisiae: A look at yeasts divided

1986 • 188 citations

Sequence of U1 RNA from Drosophila melanogaster: implications for U1 secondary structure and possible involvement in splicing

1981 • 188 citations

Role of the 3′ splice site consensus sequence in mammalian pre-mRNA splicing

1985 • 184 citations

Identification of ten genes that control ribosome formation in yeast

1970 • 182 citations

Specific and stable intron-factor interactions are established early during in vitro pre-mRNA splicing

1985 • 180 citations

Molecular consequences of specific intron mutations on yeast mRNA splicing in vivo and in vitro

1985 • 172 citations

Biochemical complementation with RNA in the Xenopus oocyte: A small rna is required for the generation of 3′ histone mRNA termini

1983 • 165 citations

Intron splicing: a conserved internal signal in introns ofDrosophilapre-mRNAs

1985 • 163 citations

U2 RNA from yeast is unexpectedly large and contains homology to vertebrate U4, U5, and U6 small nuclear RNAs

1986 • 160 citations

Fission yeast Schizosaccharomyces pombe correctly excises a mammalian RNA transcript intervening sequence

1985 • 159 citations

Splicing of adenovirus RNA in a cell-free transcription system.

1983 • 159 citations

In vivo characterization of yeast mRNA processing intermediates

1984 • 152 citations

The genes for fifteen ribosomal proteins of Saccharomyces cerevisiae.

1981 • 150 citations

Base-pairing interactions between small nuclear RNAs and nuclear RNA precursors as revealed by psoralen cross-linking in vivo

1981 • 148 citations

Compensatory mutations suggest that base-pairing with a small nuclear RNA is required to form the 3′ end of H3 messenger RNA

1986 • 146 citations

A small nuclear ribonucleoprotein associates with the AAUAAA polyadenylation signal in vitro

1986 • 139 citations

The yeast RNA gene products are essential for mRNA splicing in vitro

1986 • 138 citations

Isolation and characterization of rabbit anti-m₃^2,2,7G antibodies

1982 • 135 citations

Yeast contains small nuclear RNAs encoded by single copy genes

1983 • 133 citations

A point mutation in the conserved hexanucleotide at a yeast 5′ splice junction uncouples recognition, cleavage, and ligation

1985 • 129 citations

Specific small nuclear RNAs are associated with yeast spliceosomes

1986 • 127 citations

Cleavage of 5′ splice site and lariat formation are independent of 3′ splice site in yeast mRNA splicing

1985 • 122 citations

Small nuclear RNAs from Saccharomyces cerevisiae: unexpected diversity in abundance, size, and molecular complexity.

1986 • 105 citations

Ribonucleoprotein Complex Formation During Pre-mRNA Splicing in Vitro

1986 • 93 citations

Small Nuclear RNAs and RNA Processing

1983 • 90 citations

A Subset of Yeast snRNA's Contains Functional Binding Sites for the Highly Conserved Sm Antigen

1987 • 85 citations

Novel models for RNA splicing that involve a small nuclear RNA.

1981 • 84 citations

Deletion of a yeast small nuclear RNA gene impairs growth.

1985 • 79 citations

Monospecific antibodies reveal details of U2 snRNP structure and interaction between U1 and U2 snRNPs.

1986 • 76 citations

RNA splicing products formed with isolated fractions from HeLa cells are associated with fast-sedimenting complexes.

1986 • 75 citations

Fractionation and characterization of a yeast mRNA splicing extract.

1986 • 73 citations

Purification of a protein required for the splicing of pre-mRNA and its separation from the lariat debranching enzyme.

1985 • 72 citations

A U4-like small nuclear RNA is dispensable in yeast

1983 • 68 citations

Small Nuclear RNA U2 Is Base-Paired to Heterogeneous Nuclear RNA

1982 • 67 citations

Yeast is unable to excise foreign intervening sequences from hybrid gene transcripts.

1983 • 64 citations

Requirements for accurate and efficient mRNA 3' end cleavage and polyadenylation of a simian virus 40 early pre-RNA in vitro.

1987 • 56 citations

Yeast pre-messenger RNA splicing efficiency depends on critical spacing requirements between the branch point and 3′ splice site.

1986 • 53 citations

Isolation and characterization of two fractions from HeLa cells required for mRNA splicing in vitro.

1985 • 51 citations

Alternative branch points are selected during splicing of a yeast pre-mRNA in mammalian and yeast extracts.

1986 • 48 citations

A role for branchpoints in splicing in vivo

1985 • 48 citations

Ribonucleoprotein complex formation during pre-mRNA splicing in vitro.

1986 • 42 citations

Aspects of biochemical catalysis

1984 • 41 citations

Aberrant splicing of Drosophila alcohol dehydrogenase transcripts in Saccharomyces cerevisiae.

1983 • 38 citations

snRNP’s and scRNP’s in Eukaryotic Cells

1985 • 32 citations

Three distinct activities possibly involved in mRNA splicing are found in a nuclear fraction lacking U1 and U2 RNA

1986 • 32 citations

The enzymatic conversion of 3'-phosphate terminated RNA chains to 2',3'-cyclic phosphate derivatives.

1985 • 31 citations

Activation of a cryptic TACTAAC box in the Saccharomyces cerevisiae actin intron.

1986 • 29 citations

Yeast cell biology

1985 • 25 citations

U1 RNA-protein complex preferentially binds to both 5' and 3' splice junction sequences in RNA or single-stranded DNA.

1984 • 23 citations

Assembly in anin vitrosplicing reaction of a mouse insulin messenger RNA precursor into a 60-40S ribonucleoprotein complex

1986 • 23 citations

Ribosomal activity of the 16 S · 23 S RNA complex

1985 • 8 citations