Non-viral gene therapy

A new technique for the assay of infectivity of human adenovirus 5 DNA

1973 • 9,740 citations

High-efficiency transformation of mammalian cells by plasmid DNA.

1987 • 5,377 citations

Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure.

1987 • 4,937 citations

Direct Gene Transfer into Mouse Muscle in Vivo

1990 • 3,985 citations

Heterologous Protection Against Influenza by Injection of DNA Encoding a Viral Protein

1993 • 2,330 citations

Genetic immunization is a simple method for eliciting an immune response

1992 • 1,505 citations

Enhanced gene delivery and mechanism studies with a novel series of cationic lipid formulations.

1994 • 1,306 citations

Polyamidoamine cascade polymers mediate efficient transfection of cells in culture

1993 • 1,186 citations

DNA vaccines: protective immunizations by parenteral, mucosal, and gene-gun inoculations.

1993 • 1,122 citations

High efficiency transformation by direct microinjection of DNA into cultured mammalian cells

1980 • 1,005 citations

Cationic liposome-mediated transfection

1989 • 974 citations

Direct gene transfer with DNA-liposome complexes in melanoma: expression, biologic activity, and lack of toxicity in humans.

1993 • 888 citations

A novel cationic liposome reagent for efficient transfection of mammalian cells

1991 • 844 citations

Receptor-mediated in vitro gene transformation by a soluble DNA carrier system.

1987 • 841 citations

Long-term persistence of plasmid DNA and foreign gone expression in mouse muscle

1992 • 743 citations

Systemic Gene Expression After Intravenous DNA Delivery into Adult Mice

1993 • 725 citations

Transferrin-polycation conjugates as carriers for DNA uptake into cells.

1990 • 657 citations

Influenza virus hemagglutinin HA-2 N-terminal fusogenic peptides augment gene transfer by transferrin-polylysine-DNA complexes: toward a synthetic virus-like gene-transfer vehicle.

1992 • 657 citations

Gene inoculation generates immune responses against human immunodeficiency virus type 1.

1993 • 655 citations

Receptor-mediated gene delivery and expression in vivo.

1988 • 642 citations

In vivo and in vitro gene transfer to mammalian somatic cells by particle bombardment.

1990 • 603 citations

DNA transfection mediated by cationic liposomes containing lipopolylysine: characterization and mechanism of action

1994 • 496 citations

Human dystrophin expression in mdx mice after intramuscular injection of DNA constructs

1991 • 486 citations

Transferrin-polycation-DNA complexes: the effect of polycations on the structure of the complex and DNA delivery to cells.

1991 • 485 citations

Coupling of adenovirus to transferrin-polylysine/DNA complexes greatly enhances receptor-mediated gene delivery and expression of transfected genes.

1992 • 483 citations

Increased Expression of DNA Cointroduced with Nuclear Protein in Adult Rat Liver

1989 • 459 citations

Gene Therapy by Intramuscular Injection of Plasmid DNA: Studies on Firefly Luciferase Gene Expression in Mice

1993 • 440 citations

Protection against a lethal influenza virus challenge by immunization with a haemagglutinin-expressing plasmid DNA

1993 • 432 citations

Adenovirus enhancement of transferrin-polylysine-mediated gene delivery.

1991 • 431 citations

Introduction of foreign genes into tissues of living mice by DNA-coated microprojectiles.

1991 • 425 citations

DNA-based immunization induces continuous secretion of hepatitis B surface antigen and high levels of circulating antibody

1993 • 401 citations

Non–invasive liposome–mediated gene delivery can correct the ion transport defect in cystic fibrosis mutant mice

1993 • 400 citations

Direct transfer of transforming growth factor beta 1 gene into arteries stimulates fibrocellular hyperplasia.

1993 • 387 citations

Direct Gene Transfer into Skeletal MuscleIn Vivo: Factors Affecting Efficiency of Transfer and Stability of Expression

1993 • 380 citations

Recombinant fibroblast growth factor-1 promotes intimal hyperplasia and angiogenesis in arteries in vivo

1993 • 367 citations

Expression of recombinant genes in myocardium in vivo after direct injection of DNA.

1990 • 364 citations

Receptor-mediated gene delivery in vivo. Partial correction of genetic analbuminemia in Nagase rats

1991 • 349 citations

Transferrin-polycation-mediated introduction of DNA into human leukemic cells: stimulation by agents that affect the survival of transfected DNA or modulate transferrin receptor levels.

1990 • 340 citations

Delivery of plasmid DNA into mammalian cell lines using pH-sensitive liposomes: comparison with cationic liposomes.

1992 • 334 citations

In vivo promoter activity and transgene expression in mammalian somatic tissues evaluated by using particle bombardment.

1993 • 327 citations

Immunotherapy of malignancy by in vivo gene transfer into tumors.

1993 • 320 citations

High-efficiency receptor-mediated delivery of small and large (48 kilobase gene constructs using the endosome-disruption activity of defective or chemically inactivated adenovirus particles.

1992 • 317 citations

Plasmid DNA Is Superior to Viral Vectors for Direct Gene Transfer into Adult Mouse Skeletal Muscle

1993 • 314 citations

Gene transfer in vivo: sustained expression and regulation of genes introduced into the liver by receptor-targeted uptake.

1994 • 306 citations

Mechanism of leakage of phospholipid vesicle contents induced by the peptide GALA

1990 • 297 citations

Targeting Genes: Delivery and Persistent Expression of a Foreign Gene Driven by Mammalian Regulatory Elements in Vivo

1989 • 292 citations

Direct Gene Transfer into Nonhuman Primate Myofibers In Vivo

1992 • 284 citations

Receptor-mediated endocytosis of transferrin-polycation conjugates: an efficient way to introduce DNA into hematopoietic cells.

1990 • 275 citations

Aerosol gene delivery in vivo.

1992 • 271 citations

Gene Transfer In Vivo with DNA–Liposome Complexes: Safety and Acute Toxicity in Mice

1992 • 266 citations

Conditions affecting direct gene transfer into rodent muscle in vivo.

1991 • 262 citations

Recombinant platelet-derived growth factor B gene expression in porcine arteries induce intimal hyperplasia in vivo.

1993 • 247 citations

Hepatocyte-directed gene transfer in vivo leads to transient improvement of hypercholesterolemia in low density lipoprotein receptor-deficient rabbits.

1992 • 242 citations

Effect of cationic cholesterol derivatives on gene transfer and protein kinase C activity

1992 • 238 citations

High-Efficiency Gene Transfer Mediated by Adenovirus Coupled to DNA–Polylysine Complexes

1992 • 234 citations

Gene Transfer In Vivo with DNA–Liposome Complexes: Lack of Autoimmunity and Gonadal Localization

1992 • 222 citations

Evidence for targeted gene delivery to Hep G2 hepatoma cells in vitro

1988 • 221 citations

Use of a quaternary ammonium detergent in liposome mediated DNA transfection of mouse L-cells

1989 • 211 citations

Direct gene transfer and expression into rat heart in vivo.

1991 • 208 citations

Expression of naked plasmids by cultured myotubes and entry of plasmids into T tubules and caveolae of mammalian skeletal muscle

1992 • 206 citations

Rapid Communication: In vivo Transfection of Murine Lungs with a Functioning Prokaryotic Gene using a Liposome Vehicle

1989 • 203 citations

Examination of Parameters Affecting the Elicitation of Humoral Immune Responses by Particle Bombardment-Mediated Genetic Immunization

1993 • 188 citations

Aerosol and intravenous transfection of human alpha 1-antitrypsin gene to lungs of rabbits.

1994 • 179 citations

Expression of the human cystic fibrosis transmembrane conductance regulator gene in the mouse lung afterin vivointratracheal plasmid-mediated gene transfer

1992 • 172 citations

Safety and Short-Term Toxicity of a Novel Cationic Lipid Formulation for Human Gene Therapy

1993 • 171 citations

Hepatic gene therapy: adenovirus enhancement of receptor-mediated gene delivery and expression in primary hepatocytes.

1993 • 169 citations

Hepatic gene therapy: efficient gene delivery and expression in primary hepatocytes utilizing a conjugated adenovirus-DNA complex.

1993 • 166 citations

Fate of DNA targeted to the liver by asialoglycoprotein receptor-mediated endocytosis in vivo. Prolonged persistence in cytoplasmic vesicles after partial hepatectomy

1993 • 165 citations

Pharmacological enhancement of in vivo foreign gene expression in muscle.

1994 • 151 citations

Arterial Gene Transfer Using Pure DNA Applied Directly to a Hydrogel-Coated Angioplasty Balloon

1993 • 150 citations

Lipophilic polylysines mediate efficient DNA transfection in mammalian cells

1991 • 149 citations

Expression of hepatitis B virus surface antigen in adult rat liver. Co-introduction of DNA and nuclear protein by a simplified liposome method

1991 • 145 citations

Interaction of positively-charged liposomes with blood: implications for their application in vivo

1991 • 144 citations

DNA-binding transferrin conjugates as functional gene-delivery agents: synthesis by linkage of polylysine or ethidium homodimer to the transferrin carbohydrate moiety

1991 • 142 citations

In VivoGene Transfer into Rabbit Thyroid Follicular Cells by Direct DNA Injection

1994 • 141 citations

Introduction and Expression of the Human Insulin Gene in Adult Rat Liver

1989 • 140 citations

Folate receptor mediated DNA delivery into tumor cells: potosomal disruption results in enhanced gene expression.

1994 • 133 citations

Use of N-terminal modified poly(L-lysine)-antibody conjugate as a carrier for targeted gene delivery in mouse lung endothelial cells

1992 • 129 citations

Use of DNA Encoding Influenza Hemagglutinin as an Avian Influenza Vaccine

1993 • 125 citations

Association of a pH-sensitive peptide with membrane vesicles: role of amino acid sequence

1990 • 122 citations

Immune response to a carcinoembryonic antigen polynucleotide vaccine.

1994 • 121 citations

Persistence of plasmid DNA and expression in rat brain cells in vivo

1992 • 119 citations

Safety and Toxicity of Catheter Gene Delivery to the Pulmonary Vasculature in a Patient with Metastatic Melanoma

1994 • 108 citations

Overexpression of Human Methylmalonyl CoA Mutase in Mice afterIn VivoGene Transfer with Asialoglycoprotein/Polylysine/DNA Complexes

1994 • 104 citations

Transduction of a foreign histocompatibility gene into the arterial wall induces vasculitis.

1992 • 103 citations

Efficient and Sustained Gene Expression in Primary T Lymphocytes and Primary and Cultured Tumor Cells Mediated by Adeno-Associated Virus Plasmid DNA Complexed to Cationic Liposomes

1994 • 98 citations

Cyclic amphipathic peptide-DNA complexes mediate high-efficiency transfection of adherent mammalian cells.

1993 • 95 citations

Efficient and sustained gene expression in primary T lymphocytes and primary and cultured tumor cells mediated by adeno-associated virus plasmid DNA complexed to cationic liposomes.

1994 • 94 citations

A novel mechanism for achieving transgene persistence in vivo after somatic gene transfer into hepatocytes.

1992 • 92 citations

Incorporation of adenovirus into a ligand-based DNA carrier system results in retention of original receptor specificity and enhances targeted gene expression

1994 • 91 citations

Protection of rabbit lungs from endotoxin injury by in vivo hyperexpression of the prostaglandin G/H synthase gene.

1994 • 90 citations

Use of liposomes, viral capsids, and nanoparticles as DNA carriers

1991 • 89 citations

Rapid transgene expression in lymphocyte and macrophage primary cultures after particle bombardment-mediated gene transfer

1993 • 84 citations

Localization and Induced Expression of Fusion Genes in the Rat Lung

1991 • 80 citations

Gene transfer into somatic tissues by jet injection

1992 • 76 citations

Synthesis and characterization of a trigalactosylated bisacridine compound to target DNA to hepatocytes

1993 • 75 citations

Dystrophin expression improves myofiber survival in mdx muscle following intramuscular plasmid DNA injection

1993 • 70 citations

Direct injection of hepatitis B virus DNA into liver induced hepatitis in adult rats.

1991 • 60 citations

Transient promoter activity in primary rat mammary epithelial cells evaluated using particle bombardment gene transfer

1993 • 60 citations

Multicompartment, Numerical Model of Cellular Events in the Pharmacokinetics of Gene Therapies

1994 • 59 citations

Liposomes as carriers of DNA.

1989 • 53 citations

Utilization of modified surfactant-associated protein B for delivery of DNA to airway cells in culture.

1994 • 45 citations

Expression of a Prokaiyotic Gene in Cultured Lung Endothelial Cells after Lipofection with a Plasmid Vector

1989 • 44 citations

Biochemical and functional analysis of an adenovirus-based ligand complex for gene transfer

1994 • 44 citations

High-efficiency gene transfer employing adenovirus-polylysine-DNA complexes.

1994 • 40 citations

Expression of exogenous DNA in rat liver cells after liposome-mediated transfection in vivo

1991 • 36 citations

Expression of Human Growth Hormone Fusion Genes in Cultured Lung Endothelial Cells and in the Lungs of Mice

1993 • 36 citations

Receptor-mediated Gene Transfer to Airway Epithelial Cells in Primary Culture

1993 • 33 citations

Prolonged Transgene Expression in Rodent Lung Cells

1992 • 30 citations

Targeted transfection and expression of hepatitis B viral DNA in human hepatoma cells.

1993 • 25 citations

Genetic manipulation of rat hepatocytes in vivo. Implications for a therapy model of type-1 diabetes.

1990 • 9 citations

Pharmacokinetic Considerations in the Use of Genes as Pharmaceuticals

1994 • 4 citations