Flow cytometric measurement of intracellular cytokines

Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins.

1986 • 7,504 citations

Detection of intracellular cytokines by flow cytometry

1993 • 992 citations

Rapid Effector Function in CD8+ Memory T Cells

1997 • 652 citations

Heterogeneity of intracellular cytokine synthesis at the single-cell level in polarized T helper 1 and T helper 2 populations.

1995 • 554 citations

Selective Expression of a Stable Cell Surface Molecule on Type 2 but Not Type 1 Helper T Cells

1998 • 514 citations

Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies

1995 • 473 citations

Assessment of Cytokines by Immunofluorescence and the Paraformaldehyde‐Saponin Procedure

1991 • 465 citations

Cytotoxic T cells clear virus but augment lung pathology in mice infected with respiratory syncytial virus.

1988 • 462 citations

Reversibility of T helper 1 and 2 populations is lost after long-term stimulation.

1996 • 437 citations

Golgi-disturbing agents

1998 • 375 citations

Analysis and sorting of live cells according to secreted molecules, relocated to a cell-surface affinity matrix.

1995 • 305 citations

Modulation of Airway Inflammation by Passive Transfer of Allergen-Specific Th1 and Th2 Cells in a Mouse Model of Asthma

1999 • 273 citations

CD8⁺ T cells control Th2‐driven pathology during pulmonary respiratory syncytial virus infection

1997 • 234 citations

Intracellular Cytokine Profile of Cord and Adult Blood Lymphocytes

1998 • 223 citations

Identification of functional subsets by flow cytometry: Intracellular detection of cytokine expression

1998 • 219 citations

Quantitative intracellular cytokine measurement: age-related changes in proinflammatory cytokine production

1998 • 174 citations

Intracellular IFN-gamma expression in natural killer cells precedes lung CD8+ T cell recruitment during respiratory syncytial virus infection.

1998 • 168 citations

T Helper 1 Response Is Dominant and Localized to the Synovial Fluid in Patients with Lyme Arthritis

1998 • 167 citations

Antrum- and Corpus Mucosa-Infiltrating CD4⁺Lymphocytes inHelicobacter pyloriGastritis Display a Th1 Phenotype

1998 • 157 citations

Th1 and Th2 cytokine induction in pulmonary T cells during infection with respiratory syncytial virus

1996 • 154 citations

IL-12 Is Not Required for Induction of Type 1 Cytokine Responses in Viral Infections

1999 • 147 citations

Flow cytometric single-cell analysis of cytokine production by CD4+ T cells in synovial tissue and peripheral blood from patients with rheumatoid arthritis

1998 • 146 citations

Phorbol ester-induced downregulation of CD4 is a multistep process involving dissociation from p56lck, increased association with clathrin-coated pits, and altered endosomal sorting.

1993 • 144 citations

Single-cell cytokine analysis of gamma delta T cell responses to nonpeptide mycobacterial antigens.

1997 • 141 citations

Concomitant in vivo production of 19 different cytokines in human tonsils.

1994 • 138 citations

Regulation of T helper cell cytokine expression: functional dichotomy of antigen‐presenting cells

1993 • 136 citations

CELL-MEDIATED CYTOTOXIC RESPONSE TO RESPIRATORY SYNCYTIAL VIRUS IN INFANTS WITH BRONCHIOLITIS

1987 • 134 citations

Single cell analysis of IL-4 and IFN-gamma production by T cells from HIV-infected individuals: decreased IFN-gamma in the presence of preserved IL-4 production.

1996 • 125 citations

Cytokine flow cytometry: understanding cytokine biology at the single-cell level.

1997 • 124 citations

Demonstration of Human T Lymphotropic Virus Type I (HTLV-I) Tax-Specific CD8+ Lymphocytes Directly in Peripheral Blood of HTLV-I-Associated Myelopathy/Tropical Spastic Paraparesis Patients by Intracellular Cytokine Detection

1998 • 121 citations

Expression of pro‐inflammatory cytokines by TCRαβ⁺ T and TCRγδ⁺ T cells in an experimental model of colitis

1997 • 121 citations

Single‐cell analysis of intrathyroidal lymphocytes shows differential cytokine expression in Hashimoto's and Graves' disease

1997 • 115 citations

Decreased frequency of interferon-γ– and interleukin-2–producing cells in patients with atopic diseases measured at the single cell level

1995 • 109 citations

Expansion of Philadelphia Chromosome–Negative CD3+CD56+ Cytotoxic Cells From Chronic Myeloid Leukemia Patients: In Vitro and In Vivo Efficacy in Severe Combined Immunodeficiency Disease Mice

1998 • 109 citations

Intracellular cytokine production by human CD4+ and CD8+ T cells from normal and immunodeficient donors using directly conjugated anti-cytokine antibodies and three-colour flow cytometry

1996 • 108 citations

Interleukin‐13 is produced by activated human CD45RA⁺ and CD45R0⁺ T cells: modulation by interleukin‐4 and interleukin‐12

1996 • 103 citations

Host Genetic Determinants of Vaccine-Induced Eosinophilia During Respiratory Syncytial Virus Infection

1998 • 102 citations

Sequential production of Th1 and Th2 cytokines in response to live bacillus Calmette-Guérin.

1995 • 102 citations

Predominance of type 2 cytokine–producing CD4+ and CD8+ cells in patients with atopic dermatitis

1997 • 101 citations

In Vivo Dynamics of Anti-Viral CD8 T Cell Responses to Different Epitopes

1998 • 101 citations

Heterogeneity of the human CD4+ T-cell population: two distinct CD4+ T- cell subsets characterized by coexpression of CD45RA and CD45RO isoforms

1996 • 101 citations

Increased frequencies of interleukin-2- and interferon-gamma-producing T cells in patients with active Behçet's disease.

1998 • 99 citations

CD4+ and CD8+ T cell interactions in IFN-gamma and IL-4 responses to viral infections: requirements for IL-2.

1998 • 97 citations

Interferon-? secretion by peripheral blood T-cell subsets in multiple sclerosis: Correlation with disease phase and interferon-? therapy

1999 • 95 citations

Activated Eosinophils Are the Major Source of Th2-Associated Cytokines in the Schistosome Granuloma

1999 • 92 citations

Single-cell cytokine profiles in normal humans: Comparison of flow cytometric reagents and stimulation protocols

1997 • 86 citations

CD4+ and CD8+ T Cell Interactions in IFN-γ and IL-4 Responses to Viral Infections: Requirements for IL-2

1998 • 85 citations

Internalization, lysosomal degradation and new synthesis of surface membrane CD4 in phorbol ester-activated T-lymphocytes and U-937 cells

1992 • 85 citations

Impaired production of Th1 cytokines and increased frequency of Th2 subsets in PBMC from advanced cancer patients.

1998 • 85 citations

Type 1 and type 2 cytokine-producing mouse CD4+ and CD8+ T cells in acuteSchistosoma mansoni infection

1998 • 83 citations

CD4+and CD8+Cell Cytokine Profiles in Neonates, Older Children, and Adults: Increasing T Helper Type 1 and T Cytotoxic Type 1 Cell Populations with Age

1998 • 83 citations

Cytokine Production in Mononuclear Cells of Human Milk Studied at the Single-Cell Level

1993 • 80 citations

Opposite role for interleukin‐4 and interferon‐γ on CD30 and lymphocyte activation gene‐3 (LAG‐3) expression by activated naive T cells

1997 • 78 citations

Primary defect in CD8+ lymphocytes in the antibody deficiency disease (common variable immunodeficiency): abnormalities in intracellular production of interferon-gamma (IFN-γ) in CD28+ (‘cytotoxic’) and CD28− (‘suppressor’) CD8+ subsets

1998 • 78 citations

Internalization of human T lymphocyte receptors

1987 • 73 citations

Generation, persistence, and modulation of Th0 effector cells: role of autocrine IL-4 and IFN-gamma.

1998 • 72 citations

T1/ST2 Expression Is Enhanced on CD4+ T Cells from Schistosome Egg-Induced Granulomas: Analysis of Th Cell Cytokine Coexpression Ex Vivo

1999 • 71 citations

Th2 subset dominance among peripheral blood T lymphocytes in patients with digestive cancers

1999 • 65 citations

Determination of intracellular cytokines by flow-cytometry following whole-blood culture

1997 • 65 citations

Specific expression of surface interferon‐γ on interferon‐γ producing T cells from mouse and man

1996 • 64 citations

IL-12 treatment attenuates T helper cell type 2 and B cell responses but does not improve vaccine-enhanced lung illness.

1997 • 61 citations

IL-12 increases CD80 expression and the stimulatory capacity of bone marrow-derived dendritic cells

1998 • 61 citations

Increased intracellular Th1 cytokines in scid mice with inflammatory bowel disease

1998 • 59 citations

Analysis of type 1 and type 2 T cells in synovial fluid and peripheral blood of patients with rheumatoid arthritis.

1998 • 58 citations

Generation, Persistence, and Modulation of Th0 Effector Cells: Role of Autocrine IL-4 and IFN-γ

1998 • 56 citations

TCR αβ⁺ CD4⁻ CD8⁻ T cells differentiate extrathymically in an lck‐independent manner and participate in early response against Listeria monocytogenes infection through interferon‐γ production

1997 • 51 citations

Abnormal intracellular IL-2 and interferon-gamma (IFN-γ) production as HIV-1-associated markers of immune dysfunction

1998 • 51 citations

T-lymphocyte functionality assessed by analysis of cytokine receptor expression, intracellular cytokine expression, and femtomolar detection of cytokine secretion by quantitative flow cytometry

1998 • 51 citations

Functional Diversity of the CD8+ T-Cell Response to Epstein-Barr Virus (EBV): Implications for the Pathogenesis of EBV-Associated Lymphoproliferative Disorders

1998 • 49 citations

A cytofluorometric method for the simultaneous detection of both intracellular and surface antigens of apoptotic peripheral lymphocytes

1998 • 46 citations

Intracellular cytokine expression in whole blood preparations from normals and patients with atopic dermatitis

1997 • 45 citations

Abundant IFN-gamma production by local T cells in respiratory syncytial virus-induced eosinophilic lung disease.

1998 • 45 citations

Interferon-γ-Secreting T-Cell Populations in Rejecting Murine Cardiac Allografts

1998 • 43 citations

Cytokine production in haemodiafiltration: a multicentre study

1998 • 43 citations

Clinical Efficacy of and Immunologic Alterations Caused by Interferon g Therapy for Alveolar Echinococcosis

1998 • 43 citations

Detection of altered T helper 1 and T helper 2 cytokine production by peripheral blood mononuclear cells in patients with multiple sclerosis utilizing intracellular cytokine detection by flow cytometry and surface marker analysis

1996 • 41 citations

Frequencies of T Cells Expressing Interleukin-4 and Interleukin-5 in Atopic Asthmatic Children

1998 • 38 citations

Analysis of cytokine patterns produced by individual CD4+ lymph node cells during experimental murine leishmaniasis in resistant and susceptible mice.

1998 • 36 citations

Immunocytochemical and flow cytofluorimetric detection of intracellular IL-4, IL-5 and IFN-γ: applications using blood-and airway-derived cells

1997 • 33 citations

Single-cell entrapment and microcolony development within uniform microspheres amenable to flow cytometry

1990 • 31 citations

Degradation of CD4 following phorbol-induced internalization in human T lymphocytes. Evidence for distinct endocytic routing of CD4 and CD3.

1992 • 30 citations

Expression of selectin-binding epitopes and cytokines by CD4+ T cells repopulating scid mice with colitis

1998 • 29 citations

Immunodetection of histone epitopes correlates with early stages of apoptosis in activated human peripheral T lymphocytes.

1996 • 27 citations

Increased Type 2 Cytokine Expression by Both CD4+ CD45RO+ T Cells and CD8+ CD45RO+ T Cells in Blood Circulation is Associated with High Serum IgE but not with Atopic Dermatitis

1998 • 27 citations

Decreased frequency of interferon‐γ‐ producing CD4⁺ cells in the peripheral blood of patients with atopic dermatitis

1998 • 25 citations

Frequency and relative fraction of tumor antigen-specific T cells among lymphocytes from melanoma-invaded lymph nodes

1998 • 22 citations

Differences in the Frequency of Cytokine-Producing Cells in Antigenemic and Nonantigenemic Individuals with Bancroftian Filariasis

1998 • 22 citations

FACS-based isolation of slowly growing cells: Double encapsulation of yeast in gel microdrops

1996 • 22 citations

Cyclosporin A inhibits the decrease of CD4/CD8 expression induced by protein kinase C activation

1993 • 20 citations

Changes in Intracellular Cytokine Levels in Lymphocytes Induced by Measles Virus

1997 • 19 citations

Flow-Cytometric Assessment ofin VivoCytokine-Producing Monocytes in HIV-Infected Patients

1997 • 19 citations

Immunobiological study of interferon-gamma-producing cells after staphylococcal enterotoxin B stimulation.

1990 • 17 citations

Unstimulated basophils in atopic and nonatopic subjects express intracellular interleukin‐4: detection by flow cytometry

1998 • 15 citations

Intracellular cytokine expression in the CD4+ and CD8+ T cells from intestinal mucosa of simian immunodeficiency virus infected macaques

1998 • 15 citations

Organ culture of human lymphoid tissue. II. Marked differences in cytokine production and proliferation between slice and suspension cultures of human spleen

1997 • 15 citations

Donor cell induced CD69 expression and intracellular IL-2 and IL-4 production by peripheral blood lymphocytes isolated from kidney transplant recipients

1999 • 15 citations

Immune Determinants of Organism and Outcome in Febrile Hospitalized Thai Patients with Bloodstream Infections

1999 • 14 citations

Cloning, functional activities and in vivo tissue distribution of rat NKR-P1+ TCR alpha beta + cells

1997 • 12 citations

Determination of cytokine co‐expression in individual splenic CD4⁺ and CD8⁺ T cells from influenza virus‐immune mice

1998 • 12 citations

A comparison of the performance in vitro of precision cut tissue slices and suspensions of human spleen with special reference to immunoglobulin and cytokine production

1996 • 9 citations

Variation in Gut‐Homing CD27‐Negative Lymphocytes in Inflammatory Colon Disease

1998 • 9 citations

Murine lymph node antigen presenting cells are the main source of interleukin-6 in the initiation of delayed-type hypersensitivity.

1996 • 8 citations

Changes in intracellular cytokine levels in newborn and adult lymphocytes induced by HSV-1.

1998 • 8 citations

Changes in intracellular cytokine levels in newborn and adult lymphocytes induced by HSV-1

1998 • 6 citations

CD3⁻CD4⁻CD8⁻CD56⁻CD19⁻CD14⁻ cells and CD3⁺CD8 dull‐positive cells produce IL‐4 in AIDS patients

1998 • 3 citations

[Cell biology of bronchial asthma: interesting new methods. Intracellular determination of cytokines in T-cells and basic proteins in eosinophilic granulocytes using flow cytometry].

1997 • 1 citations