Posttranslational Protein Translocation Across the Membrane of the Endoplasmic Reticulum

A mammalian homolog of SEC61p and SECYp is associated with ribosomes and nascent polypeptides during translocation

1992 • 450 citations

BiP Acts as a Molecular Ratchet during Posttranslational Transport of Prepro-α Factor across the ER Membrane

1999 • 435 citations

A yeast mutant defective at an early stage in import of secretory protein precursors into the endoplasmic reticulum.

1987 • 418 citations

What drives the translocation of proteins?

1992 • 415 citations

Posttranslational protein transport in yeast reconstituted with a purified complex of Sec proteins and Kar2p

1995 • 400 citations

Mitochondrial Hsp70/MIM44 complex facilitates protein import

1994 • 391 citations

Sec61p and BiP directly facilitate polypeptide translocation into the ER

1992 • 370 citations

Assembly of yeast Sec proteins involved in translocation into the endoplasmic reticulum into a membrane-bound multisubunit complex

1991 • 359 citations

Alignment of Conduits for the Nascent Polypeptide Chain in the Ribosome-Sec61 Complex

1997 • 347 citations

Signal Sequence Recognition in Posttranslational Protein Transport across the Yeast ER Membrane

1998 • 343 citations

Multiple genes are required for proper insertion of secretory proteins into the endoplasmic reticulum in yeast.

1989 • 339 citations

Secretory proteins move through the endoplasmic reticulum membrane via an aqueous, gated pore

1994 • 338 citations

Protein Translocation: Tunnel Vision

1998 • 312 citations

A yeast gene important for protein assembly into the endoplasmic reticulum and the nucleus has homology to DnaJ, an Escherichia coli heat shock protein.

1989 • 296 citations

A Sec63p-BiP complex from yeast is required for protein translocation in a reconstituted proteoliposome.

1993 • 290 citations

The signal sequence moves through a ribosomal tunnel into a noncytoplasmic aqueous environment at the ER membrane early in translocation

1993 • 286 citations

Evolutionary conservation of components of the protein translocation complex

1994 • 283 citations

A posttargeting signal sequence recognition event in the endoplasmic reticulum membrane

1995 • 278 citations

Can Hsp70 proteins act as force-generating motors?

1995 • 270 citations

J Proteins Catalytically Activate Hsp70 Molecules to Trap a Wide Range of Peptide Sequences

1998 • 246 citations

The Protein Import Motor of Mitochondria

1999 • 242 citations

Topology and functional domains of Sec63p, an endoplasmic reticulum membrane protein required for secretory protein translocation.

1992 • 239 citations

Mitochondrial protein import: biochemical and genetic evidence for interaction of matrix hsp70 and the inner membrane protein MIM44.

1994 • 238 citations

Systematic probing of the environment of a translocating secretory protein during translocation through the ER membrane.

1994 • 237 citations

Signal peptides open protein-conducting channels in E. coli

1992 • 216 citations

A second trimeric complex containing homologs of the Sec61p complex functions in protein transport across the ER membrane of S. cerevisiae.

1996 • 191 citations

Dynamic interaction between Isp45 and mitochondrial hsp70 in the protein import system of the yeast mitochondrial inner membrane.

1994 • 188 citations

SEC62 encodes a putative membrane protein required for protein translocation into the yeast endoplasmic reticulum.

1989 • 186 citations

Binding of Secretory Precursor Polypeptides to a Translocon Subcomplex Is Regulated by BiP

1997 • 161 citations

Determination of the Transmembrane Topology of Yeast Sec61p, an Essential Component of the Endoplasmic Reticulum Translocation Complex

1996 • 152 citations

The Lumenal Domain of Sec63p Stimulates the ATPase Activity of BiP and Mediates BiP Recruitment to the Translocon in Saccharomyces cerevisiae

1997 • 151 citations

Evidence for the loop model of signal-sequence insertion into the endoplasmic reticulum.

1988 • 148 citations

Protein Transport by Purified Yeast Sec Complex and Kar2p Without Membranes

1997 • 146 citations

Genetic interactions between KAR2 and SEC63, encoding eukaryotic homologues of DnaK and DnaJ in the endoplasmic reticulum.

1993 • 135 citations

Structural and functional dissection of Sec62p, a membrane-bound component of the yeast endoplasmic reticulum protein import machinery.

1990 • 121 citations

Identification of the secY (prlA) gene product involved in protein export in Escherichia coli

1984 • 119 citations

Interaction between BiP and Sec63p is required for the completion of protein translocation into the ER of Saccharomyces cerevisiae.

1995 • 113 citations

The yeast SSS1 gene is essential for secretory protein translocation and encodes a conserved protein of the endoplasmic reticulum.

1993 • 107 citations

Mutants in three novel complementation groups inhibit membrane protein insertion into and soluble protein translocation across the endoplasmic reticulum membrane of Saccharomyces cerevisiae.

1992 • 102 citations

Unfolding of preproteins upon import into mitochondria

1998 • 100 citations

Structural and functional characterization of Sec66p, a new subunit of the polypeptide translocation apparatus in the yeast endoplasmic reticulum.

1993 • 86 citations

The mitochondrial protein import motor: Dissociation of mitochondrial hsp70 from its membrane anchor requires ATP binding rather than ATP hydrolysis

1996 • 84 citations

ER membrane protein complex required for nuclear fusion.

1996 • 80 citations

Signal Sequence Recognition in Cotranslational Translocation by Protein Components of the Endoplasmic Reticulum Membrane

1998 • 80 citations

The PrlA and PrlG phenotypes are caused by a loosened association among the translocase SecYEG subunits

1999 • 78 citations

The allele-specific synthetic lethality of prlA-prlG double mutants predicts interactive domains of SecY and SecE.

1995 • 73 citations

Residues essential for the function of SecE, a membrane component of the Escherichia coli secretion apparatus, are located in a conserved cytoplasmic region.

1994 • 68 citations

Molecular architecture of the ER translocase probed by chemical crosslinking of Sss1p to complementary fragments of Sec61p

1997 • 57 citations

Secretion in yeast: preprotein binding to a membrane receptor and ATP-dependent translocation are sequential and separable events in vitro.

1989 • 40 citations

Protein translocation genetics

1995 • 14 citations

Human Sec63 endoplasmic reticulum membrane protein, map position 6q21.

1999 • 3 citations