Vigilance and Validation: Keys to Success in RNAi Screening

MicroRNAs: Target Recognition and Regulatory Functions

2009 • 19,026 citations

Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans

1998 • 14,674 citations

Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells

2001 • 9,275 citations

Most mammalian mRNAs are conserved targets of microRNAs

2008 • 8,043 citations

Origins and Mechanisms of miRNAs and siRNAs

2009 • 4,724 citations

miRBase: tools for microRNA genomics

2007 • 4,369 citations

MicroRNA Targeting Specificity in Mammals: Determinants beyond Seed Pairing

2007 • 3,708 citations

Regulation of mRNA Translation and Stability by microRNAs

2010 • 2,958 citations

Transcriptome-wide Identification of RNA-Binding Protein and MicroRNA Target Sites by PAR-CLIP

2010 • 2,785 citations

microRNA Functions

2007 • 2,524 citations

Introduction of a Chimeric Chalcone Synthase Gene into Petunia Results in Reversible Co-Suppression of Homologous Genes in trans.

1990 • 2,386 citations

Expression profiling reveals off-target gene regulation by RNAi

2003 • 2,294 citations

MicroRNA sponges: competitive inhibitors of small RNAs in mammalian cells

2007 • 2,024 citations

Functional genomic analysis of C. elegans chromosome I by systematic RNA interference

2000 • 1,767 citations

A Lentiviral RNAi Library for Human and Mouse Genes Applied to an Arrayed Viral High-Content Screen

2006 • 1,752 citations

Argonaute HITS-CLIP decodes microRNA–mRNA interaction maps

2009 • 1,732 citations

Fatality in mice due to oversaturation of cellular microRNA/short hairpin RNA pathways

2006 • 1,633 citations

Identification of Host Proteins Required for HIV Infection Through a Functional Genomic Screen

2008 • 1,424 citations

Activation of the interferon system by short-interfering RNAs

2003 • 1,416 citations

siRNAs can function as miRNAs

2003 • 1,252 citations

Flavonoid genes in petunia: addition of a limited number of gene copies may lead to a suppression of gene expression.

1990 • 1,182 citations

Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA

2005 • 1,177 citations

A Genome-wide RNAi Screen Identifies Multiple Synthetic Lethal Interactions with the Ras Oncogene

2009 • 982 citations

Induction of an interferon response by RNAi vectors in mammalian cells

2003 • 972 citations

Introduction of a Chimeric Chalcone Synthase Gene into Petunia Results in Reversible Co-Suppression of Homologous Genes in trans

1990 • 962 citations

Recognizing and avoiding siRNA off-target effects for target identification and therapeutic application

2009 • 953 citations

Perspective: machines for RNAi

2005 • 942 citations

Global Analysis of Host-Pathogen Interactions that Regulate Early-Stage HIV-1 Replication

2008 • 941 citations

Widespread siRNA “off-target” transcript silencing mediated by seed region sequence complementarity

2006 • 916 citations

Sequence- and target-independent angiogenesis suppression by siRNA via TLR3

2008 • 892 citations

Functional genomic analysis of cell division in C. elegans using RNAi of genes on chromosome III

2000 • 867 citations

3′ UTR seed matches, but not overall identity, are associated with RNAi off-targets

2006 • 865 citations

Genome-Scale RNAi Screen for Host Factors Required for HIV Replication

2008 • 760 citations

Position-specific chemical modification of siRNAs reduces “off-target” transcript silencing

2006 • 758 citations

Genome-Wide RNAi Analysis of Growth and Viability in Drosophila Cells

2004 • 699 citations

Genome-Wide RNAi of C. elegans Using the Hypersensitive rrf-3 Strain Reveals Novel Gene Functions

2003 • 635 citations

Biological functions of microRNAs: a review

2010 • 609 citations

Short interfering RNAs can induce unexpected and divergent changes in the levels of untargeted proteins in mammalian cells

2004 • 600 citations

Expanding the MicroRNA Targeting Code: Functional Sites with Centered Pairing

2010 • 581 citations

Highly parallel identification of essential genes in cancer cells

2008 • 556 citations

Nonspecific, concentration-dependent stimulation and repression of mammalian gene expression by small interfering RNAs (siRNAs)

2003 • 530 citations

Dicing and slicing

2005 • 525 citations

Transfection of small RNAs globally perturbs gene regulation by endogenous microRNAs

2009 • 510 citations

RNA interference screen for human genes associated with West Nile virus infection

2008 • 507 citations

A systematic RNAi screen for longevity genes in C. elegans

2005 • 505 citations

Specificity of short interfering RNA determined through gene expression signatures

2003 • 497 citations

Off-target effects by siRNA can induce toxic phenotype

2006 • 468 citations

Synthetic lethal screen identification of chemosensitizer loci in cancer cells

2007 • 466 citations

Minimizing the risk of reporting false positives in large-scale RNAi screens

2006 • 424 citations

A Genome-wide RNAi Screen for Modifiers of the Circadian Clock in Human Cells

2009 • 421 citations

A Functional Genomic Screen Identifies Cellular Cofactors of Hepatitis C Virus Replication

2009 • 418 citations

An endoribonuclease-prepared siRNA screen in human cells identifies genes essential for cell division

2004 • 394 citations

A functional RNAi screen links O-GlcNAc modification of ribosomal proteins to stress granule and processing body assembly

2008 • 381 citations

High-throughput RNAi screening in cultured cells: a user's guide

2006 • 375 citations

Molecular characterization of human Argonaute-containing ribonucleoprotein complexes and their bound target mRNAs

2008 • 365 citations

siRNA-mediated off-target gene silencing triggered by a 7 nt complementation

2005 • 354 citations

Activation of the mammalian immune system by siRNAs

2005 • 353 citations

A genome-wide genetic screen for host factors required for hepatitis C virus propagation

2009 • 350 citations

Target mRNA abundance dilutes microRNA and siRNA activity

2010 • 338 citations

Functional Genomic Analysis of the Wnt-Wingless Signaling Pathway

2005 • 334 citations

Parallel Chemical Genetic and Genome-Wide RNAi Screens Identify Cytokinesis Inhibitors and Targets

2004 • 320 citations

Profiling Essential Genes in Human Mammary Cells by Multiplex RNAi Screening

2008 • 303 citations

Identification of JAK/STAT signalling components by genome-wide RNA interference

2005 • 293 citations

Genomewide view of gene silencing by small interfering RNAs

2003 • 287 citations

Genomic Screening with RNAi: Results and Challenges

2010 • 281 citations

Detecting microRNA binding and siRNA off-target effects from expression data

2008 • 270 citations

Functional Genomic Analysis of RNA Interference in C. elegans

2005 • 268 citations

A Genome-wide Short Hairpin RNA Screening of Jurkat T-cells for Human Proteins Contributing to Productive HIV-1 Replication

2009 • 260 citations

Functional Dissection of an Innate Immune Response by a Genome-Wide RNAi Screen

2004 • 240 citations

Identification of genes that protect theC. elegansgenome against mutations by genome-wide RNAi

2003 • 211 citations

Genome‐wide screening for gene function using RNAi in mammalian cells

2005 • 210 citations

A Genome-Wide Screen Identifies 27 Genes Involved in Transposon Silencing in C. elegans

2003 • 198 citations

Sequence-non-specific effects of RNA interference triggers and microRNA regulators

2009 • 198 citations

Improved silencing properties using small internally segmented interfering RNAs

2007 • 186 citations

Transgenic microRNA inhibition with spatiotemporal specificity in intact organisms

2009 • 179 citations

Innate sensing of self and non-self RNAs by Toll-like receptors

2006 • 175 citations

Functional dissection of siRNA sequence by systematic DNA substitution: modified siRNA with a DNA seed arm is a powerful tool for mammalian gene silencing with significantly reduced off-target effect

2008 • 168 citations

A genome-wide RNAi screen for Wnt/β-catenin pathway components identifies unexpected roles for TCF transcription factors in cancer

2008 • 167 citations

A screen of chemical modifications identifies position-specific modification by UNA to most potently reduce siRNA off-target effects

2010 • 166 citations

A genome-wide RNA interference screen reveals an essential CREB3L2-ATF5-MCL1 survival pathway in malignant glioma with therapeutic implications

2010 • 154 citations

Genome-Wide siRNA-Based Functional Genomics of Pigmentation Identifies Novel Genes and Pathways That Impact Melanogenesis in Human Cells

2008 • 151 citations

New Regulators of Wnt/β-Catenin Signaling Revealed by Integrative Molecular Screening

2008 • 150 citations

Experimental validation of the importance of seed complement frequency to siRNA specificity

2008 • 140 citations

A Large Scale shRNA Barcode Screen Identifies the Circadian Clock Component ARNTL as Putative Regulator of the p53 Tumor Suppressor Pathway

2009 • 135 citations

Small interfering RNAs containing full 2′-O-methylribonucleotide-modified sense strands display Argonaute2/eIF2C2-dependent activity

2005 • 133 citations

Genome-wide functional analysis of human cell-cycle regulators

2006 • 131 citations

Asymmetric RNA duplexes mediate RNA interference in mammalian cells

2008 • 115 citations

A functional genomic screen identifies a role for TAO1 kinase in spindle-checkpoint signalling

2007 • 94 citations

RNA interference rescue by bacterial artificial chromosome transgenesis in mammalian tissue culture cells

2005 • 91 citations

Off-target effects of siRNA specific for GFP

2008 • 87 citations

MicroRNA-like off-target transcript regulation by siRNAs is species specific

2009 • 80 citations

Recent Progress in Chemically Modified siRNAs

2010 • 76 citations

Selection of hyperfunctional siRNAs with improved potency and specificity

2009 • 66 citations

Re-examination of siRNA specificity questions role of PICH and Tao1 in the spindle checkpoint and identifies Mad2 as a sensitive target for small RNAs

2009 • 62 citations

Cellular phenotyping by RNAi

2006 • 45 citations

A functional genome-wide RNAi screen identifies TAF1 as a regulator for apoptosis in response to genotoxic stress

2008 • 44 citations

Systematic analysis of off-target effects in an RNAi screen reveals microRNAs affecting sensitivity to TRAIL-induced apoptosis

2010 • 43 citations

RNAi screen for telomerase reverse transcriptase transcriptional regulators identifies HIF1α as critical for telomerase function in murine embryonic stem cells

2010 • 42 citations

Functional Dissection of Mitotic Regulators Through Gene Targeting in Human Somatic Cells

2009 • 35 citations

An RNAi-based genetic screen for oxidative stress resistance reveals retinol saturase as a mediator of stress resistance

2007 • 34 citations

Functional Genomic Analysis of Cell Division by Endoribonuclease-Prepared siRNAs

2005 • 34 citations

Increasing the Robustness and Validity of RNAi Screens

2007 • 33 citations

From sequence to function: using RNAi to elucidate mechanisms of human disease

2008 • 30 citations

Minimizing off-target effects by using diced siRNAs for RNA interference.

2006 • 29 citations

miRNAs: small changes, widespread effects

2008 • 25 citations

Small molecules in an RNAi world

2005 • 22 citations

The best control for the specificity of RNAi

2005 • 22 citations

Systems biology of mammalian cell division

2008 • 16 citations

Systems analysis of quantitative shRNA-library screens identifies regulators of cell adhesion

2008 • 14 citations