E unum pluribus: multiple proteins from a self-processing polyprotein

Foot-and-Mouth Disease

2004 • 1,696 citations

Analysis of the aphthovirus 2A/2B polyprotein ‘cleavage’ mechanism indicates not a proteolytic reaction, but a novel translational effect: a putative ribosomal ‘skip’

2001 • 761 citations

The ‘cleavage’ activities of foot-and-mouth disease virus 2A site-directed mutants and naturally occurring ‘2A-like’ sequences

2001 • 507 citations

The N-end rule

1992 • 464 citations

Cleavage of foot-and-mouth disease virus polyprotein is mediated by residues located within a 19 amino acid sequence

1991 • 457 citations

Gene stacking in transgenic plants – the challenge for 21st century plant biotechnology

2005 • 395 citations

Nascent Membrane and Secretory Proteins Differ in FRET-Detected Folding Far inside the Ribosome and in Their Exposure to Ribosomal Proteins

2004 • 368 citations

Foot-and-mouth disease virus 2A oligopeptide mediated cleavage of an artificial polyprotein.

1994 • 367 citations

Stable antibody expression at therapeutic levels using the 2A peptide

2005 • 365 citations

Coordinate dual-gene transgenesis by lentiviral vectors carrying synthetic bidirectional promoters

2004 • 318 citations

Development of 2A peptide-based strategies in the design of multicistronic vectors

2005 • 272 citations

Phloem Unloading in Sink Leaves of Nicotiana benthamiana: Comparison of a Fluorescent Solute with a Fluorescent Virus.

1997 • 267 citations

Two-Helper RNA System for Production of Recombinant Semliki Forest Virus Particles

1999 • 258 citations

Assembly and movement of a plant virus carrying a green fluorescent protein overcoat.

1996 • 257 citations

Both Lumenal and Cytosolic Gating of the Aqueous ER Translocon Pore Are Regulated from Inside the Ribosome during Membrane Protein Integration

1997 • 255 citations

The cleavage activities of aphthovirus and cardiovirus 2A proteins.

1997 • 172 citations

Co-translational, Intraribosomal Cleavage of Polypeptides by the Foot-and-mouth Disease Virus 2A Peptide

2003 • 167 citations

Metabolic engineering of ketocarotenoid formation in higher plants

2004 • 165 citations

High-level ectopic HOXB4 expression confers a profound in vivo competitive growth advantage on human cord blood CD34+ cells, but impairs lymphomyeloid differentiation

2003 • 162 citations

Self‐processing 2A‐polyproteins – a system for co‐ordinate expression of multiple proteins in transgenic plants

1999 • 121 citations

Engineering Cowpea Mosaic Virus RNA-2 into a Vector to Express Heterologous Proteins in Plants

2000 • 113 citations

Stable High-Level Expression of Heterologous Genes In Vitro and In Vivo by Noncytopathic DNA-Based Kunjin Virus Replicon Vectors

2000 • 109 citations

Subgenomic Hepatitis C Virus Replicons Inducing Expression of a Secreted Enzymatic Reporter Protein

2002 • 103 citations

Polycistronic Viral Vectors

2002 • 99 citations

Recombinant AAV vectors containing the foot and mouth disease virus 2A sequence confer efficient bicistronic gene expression in cultured cells and rat substantia nigra neurons

2001 • 99 citations

Replicating Foamy Virus-Based Vectors Directing High Level Expression of Foreign Genes

1995 • 97 citations

Targeting of Proteins Derived from Self‐Processing Polyproteins Containing Multiple Signal Sequences

2004 • 96 citations

Retrovirally Delivered Random Cyclic Peptide Libraries Yield Inhibitors of Interleukin-4 Signaling in Human B Cells

2002 • 95 citations

Use of the 2A sequence from foot-and-mouth disease virus in the generation of retroviral vectors for gene therapy

1999 • 94 citations

Molecular Imaging Using Labeled Donor Tissues Reveals Patterns of Engraftment, Rejection, and Survival in Transplantation

2005 • 94 citations

Expression of a foreign protein by influenza A virus

1994 • 89 citations

Core Protein-Coding Sequence, but Not Core Protein, Modulates the Efficiency of Cap-Independent Translation Directed by the Internal Ribosome Entry Site of Hepatitis C Virus

2000 • 88 citations

HOXB4 enforces equivalent fates of ES-cell-derived and adult hematopoietic cells

2005 • 85 citations

Rotavirus VP6 Expressed by PVX Vectors in Nicotiana benthamiana Coats PVX Rods and Also Assembles into Viruslike Particles

2000 • 84 citations

Semliki Forest Virus Vectors Engineered to Express Higher IL-12 Levels Induce Efficient Elimination of Murine Colon Adenocarcinomas

2005 • 84 citations

Enabling technologies for manipulating multiple genes on complex pathways

2001 • 81 citations

Development of detection methods for ruminant interleukin (IL)-4

2005 • 76 citations

Noncytopathic Flavivirus Replicon RNA-Based System for Expression and Delivery of Heterologous Genes

1999 • 76 citations

Kunjin Virus Replicon Vaccine Vectors Induce Protective CD8⁺T-Cell Immunity

2002 • 75 citations

Coordinate Expression and Independent Subcellular Targeting of Multiple Proteins from a Single Transgene

2004 • 74 citations

Rapid identification of coronavirus replicase inhibitors using a selectable replicon RNA

2004 • 74 citations

Retroviral vector-mediated expression of HoxB4 in hematopoietic cells using a novel coexpression strategy

2001 • 72 citations

A Model for Nonstoichiometric, Cotranslational Protein Scission in Eukaryotic Ribosomes

1999 • 70 citations

Production of a functional single chain antibody attached to the surface of a plant virus

1998 • 68 citations

Inducible expression of the P, V, and NP genes of the paramyxovirus simian virus 5 in cell lines and an examination of NP-P and NP-V interactions

1995 • 68 citations

Untitled

2001 • 67 citations

A picornaviral 2A-like sequence-based tricistronic vector allowing for high-level therapeutic gene expression coupled to a dual-reporter system

2005 • 63 citations

Actively replicating West Nile virus is resistant to cytoplasmic delivery of siRNA

2005 • 63 citations

Limitations on the use of fused green fluorescent protein to investigate structure–function relationships for the cauliflower mosaic virus movement protein

2000 • 61 citations

Use and comparison of different internal ribosomal entry sites (IRES) in tricistronic retroviral vectors.

2004 • 61 citations

Sindbis Virus Vectors Designed To Express a Foreign Protein as a Cleavable Component of the Viral Structural Polyprotein

2003 • 60 citations

Essential and Dispensable Virus-Encoded Replication Elements Revealed by Efforts To Develop Hypoviruses as Gene Expression Vectors

2000 • 58 citations

Metabolic engineering in the post genomic era

2004 • 56 citations

Expression of a Foreign Epitope by Porcine Reproductive and Respiratory Syndrome Virus

2000 • 53 citations

Tumor imaging using a standardized radiolabeled adapter protein docked to vascular endothelial growth factor.

2004 • 52 citations

Stacking transgenes in forest trees

2003 • 52 citations

Intrinsic direct repeats generate consistent post‐transcriptional gene silencing in tobacco

2002 • 52 citations

Coexpression of Interleukin-12 Chains by a Self-Splicing Vector Increases the Protective Cellular Immune Response of DNA andMycobacterium bovisBCG Vaccines againstMycobacterium tuberculosis

2002 • 52 citations

Kunjin Virus Replicon Vectors for Human Immunodeficiency Virus Vaccine Development

2003 • 51 citations

Cells Expressing the Human Foamy Virus (HFV) Accessory Bet Protein Are Resistant to Productive HFV Superinfection

1998 • 49 citations

Capsid Processing Requirements for Abrogation of Fv1 and Ref1 Restriction

2005 • 49 citations

Engineering of Alfalfa mosaic virus RNA 3 into an expression vector

2001 • 49 citations

Processing in Arabidopsis thaliana of a heterologous polyprotein resulting in differential targeting of the individual plant defensins

2003 • 47 citations

The C terminus of the movement protein of Brome mosaic virus controls the requirement for coat protein in cell-to-cell movement and plays a role in long-distance movement

2004 • 46 citations

Production of Interleukin-12 as a Self-Processing 2A Polypeptide

1999 • 45 citations

FMDV–2A sequence and protein arrangement contribute to functionality of CYP2B1–reporter fusion protein

2005 • 45 citations

Foamy virus vectors for suicide gene therapy

1997 • 43 citations

Comparative affects of plasmid‐encoded interleukin 12 and interleukin 18 on the protective efficacy of DNA vaccination against Mycobacterium tuberculosis

2002 • 42 citations

Induction of Cellular Immune Responses to Simian Immunodeficiency Virus Gag by Two Recombinant Negative-Strand RNA Virus Vectors

2004 • 40 citations

Untitled

2002 • 38 citations

Diabetes Incidence Is Unaltered in Glutamate Decarboxylase 65-Specific TCR Retrogenic Nonobese Diabetic Mice: Generation by Retroviral-Mediated Stem Cell Gene Transfer

2004 • 38 citations

Tricistronic and Tetracistronic Retroviral Vectors for Gene Transfer

2000 • 37 citations

Enhanced in vivo selection of bone marrow cells by retroviral-mediated coexpression of mutant O6-methylguanine-DNA-methyltransferase and HOXB4

2004 • 34 citations

Stable, stoichiometric delivery of diverse protein functions

2003 • 29 citations

Foot-and-mouth disease virus 2A protease mediates cleavage in attenuated Sabin 3 poliovirus vectors engineered for delivery of foreign antigens

1996 • 29 citations

Enhanced cellular immune responses to SIV Gag by immunization with influenza and vaccinia virus recombinants

2003 • 29 citations

Deletion Mapping of the Encephalomyocarditis Virus Primary Cleavage Site

2001 • 29 citations

Development of dengue virus replicons expressing HIV-1 gp120 and other heterologous genes: a potential future tool for dual vaccination against dengue virus and HIV.

2001 • 28 citations

Repetitive Intrathecal Injections of Poliovirus Replicons Result in Gene Expression in Neurons of the Central Nervous System Without Pathogenesis

2001 • 27 citations

Influence of IL-12 on interferon-γ production by bovine leucocyte subsets in response to bovine respiratory syncytial virus

1998 • 26 citations

The combination of plasmid interleukin‐12 with a single DNA vaccine is more effective than Mycobacterium bovis (bacille Calmette–Guèrin) in protecting against systemic Mycobacterim avium infection

2003 • 26 citations

Involvement of a Bovine Viral Diarrhea Virus NS5B Locus in Virion Assembly

2004 • 26 citations

Subcellular location of the helper component-proteinase of Cowpea aphid-borne mosaic virus.

2002 • 25 citations

RNA replicons derived from poliovirus are directly oncolytic for human tumor cells of diverse origins.

2001 • 24 citations

Production of biologically active, heterodimeric porcine interleukin-12 using a monocistronic baculoviral expression system

1999 • 22 citations

Ovine Interleukin-12: Analysis of Biologic Function and Species Comparison

2000 • 22 citations

Co-ordinate expression of β- and δ-zeins in transgenic tobacco

2004 • 20 citations

The Aphtho- and Cardiovirus “Primary” 2A/2B Polyprotein “Cleavage”

2014 • 18 citations

Dual role of the adenovirus pVI C terminus as a nuclear localization signal and activator of the viral protease

2004 • 18 citations

Gene expression in the muscle and central nervous system following intramuscular inoculation of encapsidated or naked poliovirus replicons

2003 • 16 citations

Intranasal inoculation of a recombinant influenza virus containing exogenous nucleotides in the NS segment induces mucosal immune response against the exogenous gene product in mice

2002 • 15 citations

Construction and characterization of pentacistronic retrovirus vectors

2003 • 14 citations

Genetic stability of attenuated mengovirus vectors with duplicate primary cleavage sequences

2003 • 14 citations

Genetic Engineering of Drought Resistant Potato Plants by Co-Introduction of Genes Encoding Trehalose-6-Phosphate Synthase and Trehalose-6-Phosphate Phosphatase of Zygosaccharomyces rouxii

2004 • 12 citations

Foot-and-Mouth Disease Virus Proteinases

2019 • 12 citations

Buffalo (Bubalus bubalis) interleukin-12: Analysis of expression profiles and functional cross-reactivity with bovine system

2005 • 9 citations

Intramuscular Immunization with Poliovirus Replicons Primes for a Humoral and Cellular Immune Response to Soluble Antigen

2003 • 6 citations

Design and Efficacy of a Gene Trap System for Secretory Proteins

2004 • 1 citations