Site directed mutagenesis of DNA polymerase I (Klenow) from Escherichia coli

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Current protocols in molecular biology

1988 • 4,543 citations

Vectors for selective expression of cloned DNAs by T7 RNA polymerase

1987 • 1,449 citations

The "megaprimer" method of site-directed mutagenesis.

1990 • 1,174 citations

Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase

1988 • 814 citations

Enzymatic Synthesis of Deoxyribonucleic Acid

1969 • 543 citations

Identification of residues critical for the polymerase activity of the Klenow fragment of DNA polymerase I from Escherichia coli.

1990 • 261 citations

Nucleotide sequence of the Escherichia coli polA gene and primary structure of DNA polymerase I.

1982 • 169 citations

Construction of a plasmid that overproduces the large proteolytic fragment (Klenow fragment) of DNA polymerase I of Escherichia coli.

1983 • 168 citations

How DNA Travels between the Separate Polymerase and 3′-5′-Exonuclease Sites of DNA Polymerase I (Klenow Fragment)

1989 • 129 citations

[9] The kinetics of immobilized enzyme systems

1980 • 97 citations

Identification and amino acid sequence of the deoxynucleoside triphosphate binding site in Escherichia coli DNA polymerase I

1987 • 73 citations

Photoaffinity labeling of the thymidine triphosphate binding domain in Escherichia coli DNA polymerase I: identification of histidine-881 as the site of cross-linking

1987 • 59 citations

Photoaffinity labeling of the Klenow fragment with 8-azido-dATP.

1990 • 58 citations

"Megaprimer" method of PCR: increased template concentration improves yield.

1991 • 56 citations

Mechanism of hydrolysis of cyclic phosphate esters

1969 • 38 citations

Affinity labeling of Escherichia coli DNA polymerase I by 5'-fluorosulfonylbenzoyladenosine. Identification of the domain essential for polymerization and Arg-682 as the site of reactivity.

1988 • 32 citations

Pyridoxal 5'-phosphate mediated inactivation of Escherichia coli DNA polymerase I: identification of lysine-635 as an essential residue for the processive mode of DNA synthesis

1988 • 29 citations

A molecular model of the complete three-dimensional structure of the Klenow fragment of Escherichia coli DNA polymerase I: binding of the dNTP substrate and template-primer

1992 • 24 citations

Terminal deoxynucleotidyltransferase containing megadalton complex from young rat thymus nuclei: identification and characterization

1990 • 22 citations

Extracellular and intracellular nucleases in Staphylococcus aureus.

1989 • 1 citations