Recent developments in the molecular, biochemical and functional characterization of GPI8 and the GPI-anchoring mechanism [Review]

A new method for predicting signal sequence cleavage sites

1986 • 4,629 citations

Functional Characterization of the S. cerevisiae Genome by Gene Deletion and Parallel Analysis

1999 • 3,929 citations

MEROPS: the peptidase database

1999 • 1,939 citations

The Genome of the African Trypanosome Trypanosoma brucei

2005 • 1,617 citations

The Genome of the Kinetoplastid Parasite, Leishmania major

2005 • 1,324 citations

The Genome Sequence of Trypanosoma cruzi , Etiologic Agent of Chagas Disease

2005 • 1,321 citations

The structure, biosynthesis and function of glycosylated phosphatidylinositols in the parasitic protozoa and higher eukaryotes

1993 • 896 citations

The structure, biosynthesis and functions of glycosylphosphatidylinositol anchors, and the contributions of trypanosome research

1999 • 622 citations

A new generation of information retrieval tools for biologists: the example of the ExPASy WWW server

1994 • 564 citations

MEROPS: the peptidase database

2005 • 486 citations

HOW GLYCOSYL-PHOSPHATIDYLINOSITOL-ANCHORED MEMBRANE PROTEINS ARE MADE

1995 • 478 citations

Signal sequences specify the targeting route to the endoplasmic reticulum membrane.

1996 • 455 citations

Cloning, Isolation, and Characterization of Mammalian Legumain, an Asparaginyl Endopeptidase

1997 • 368 citations

[32] Families of cysteine peptidases

1994 • 362 citations

An asparaginyl endopeptidase processes a microbial antigen for class II MHC presentation

1998 • 362 citations

MEROPS: the peptidase database

2003 • 360 citations

Lipophosphoglycan is a virulence factor distinct from related glycoconjugates in the protozoan parasiteLeishmania major

2000 • 298 citations

Tissue-specific knockout of the mouse Pig-a gene reveals important roles for GPI-anchored proteins in skin development

1997 • 263 citations

Targeted gene deletion in Leishmania major identifies leishmanolysin (GP63) as a virulence factor

2002 • 253 citations

Secretory Pathway of Trypanosomatid Parasites

2002 • 247 citations

Surface antigens of Toxoplasma gondii: variations on a theme

2001 • 241 citations

Glycosylphosphatidylinositol-anchor-deficient mice: implications for clonal dominance of mutant cells in paroxysmal nocturnal hemoglobinuria

1996 • 238 citations

Determinants for glycophospholipid anchoring of the Saccharomyces cerevisiae GAS1 protein to the plasma membrane.

1991 • 222 citations

Identification of the active site of legumain links it to caspases, clostripain and gingipains in a new clan of cysteine endopeptidases

1998 • 221 citations

Phosphoinositides and signal transduction

2002 • 219 citations

Phosphatidylinositol glycan (PI-G) anchored membrane proteins. Amino acid requirements adjacent to the site of cleavage and PI-G attachment in the COOH-terminal signal peptide.

1992 • 211 citations

Mammalian GPI proteins: sorting, membrane residence and functions

1997 • 206 citations

Vacuolar Processing Enzymes Are Essential for Proper Processing of Seed Storage Proteins in Arabidopsis thaliana

2003 • 199 citations

The role of phosphoglycans inLeishmania–sand fly interactions

2000 • 198 citations

Dissecting and manipulating the pathway for glycosylphos-phatidylinositol-anchor biosynthesis

2000 • 189 citations

Structures of glycosylphosphatidylinositol membrane anchors from Saccharomyces cerevisiae.

1993 • 186 citations

The Two Cysteine Endopeptidases of Legume Seeds: Purification and Characterization by Use of Specific Fluorometric Assays

1993 • 180 citations

PIG-S and PIG-T, essential for GPI anchor attachment to proteins, form a complex with GAA1 and GPI8

2001 • 176 citations

Yeast Gpi8p is essential for GPI anchor attachment onto proteins.

1996 • 176 citations

Critical roles of glycosylphosphatidylinositol for Trypanosoma brucei

2000 • 171 citations

Is lipophosphoglycan a virulence factor? A surprising diversity between Leishmania species

2001 • 171 citations

Persistence Without Pathology in Phosphoglycan-Deficient Leishmania major

2003 • 170 citations

Yeast Gaa1p is required for attachment of a completed GPI anchor onto proteins.

1995 • 170 citations

Enzymes and auxiliary factors for GPI lipid anchor biosynthesis and post‐translational transfer to proteins

2003 • 168 citations

Early lipid intermediates in glycosyl-phosphatidylinositol anchor assembly are synthesized in the ER and located in the cytoplasmic leaflet of the ER membrane bilayer.

1993 • 143 citations

The surface glycoconjugates of trypanosomatid parasites

1997 • 138 citations

Asparaginyl endopeptidase of jack bean seeds. Purification, characterization, and high utility in protein sequence analysis.

1993 • 137 citations

Lipophosphoglycan is not required for infection of macrophages or mice by Leishmania mexicana

2000 • 134 citations

Glycosyl-phosphatidylinositol Membrane Anchors: The Tale of a Tail

1992 • 133 citations

Analysis of the sequence requirements for glycosylphosphatidylinositol anchoring of Saccharomyces cerevisiae Gas1 protein

1993 • 130 citations

Gaa1p and Gpi8p Are Components of a Glycosylphosphatidylinositol (GPI) Transamidase That Mediates Attachment of GPI to Proteins

2000 • 129 citations

Biosynthesis of glycosylphosphatidylinositol (GPI)-anchored membrane proteins in intact cells: specific amino acid requirements adjacent to the site of cleavage and GPI attachment.

1993 • 127 citations

MCD4Encodes a Conserved Endoplasmic Reticulum Membrane Protein Essential for Glycosylphosphatidylinositol Anchor Synthesis in Yeast

1999 • 126 citations

Temperature-sensitive Yeast GPI Anchoring Mutants gpi2 and gpi3 Are Defective in the Synthesis of N-Acetylglucosaminyl Phosphatidylinositol.

1995 • 123 citations

Human PIG-U and Yeast Cdc91p Are the Fifth Subunit of GPI Transamidase That Attaches GPI-Anchors to Proteins

2003 • 120 citations

The GPI anchor of cell-surface proteins is synthesized on the cytoplasmic face of the endoplasmic reticulum.

1994 • 120 citations

CATALYSIS OF TRANSAMIDATION REACTIONS BY PROTEOLYTIC ENZYMES

1950 • 120 citations

Targeted gene deletion of Leishmania major genes encoding developmental stage‐specific leishmanolysin (GP63)

1998 • 118 citations

Requirements for glycosylphosphatidylinositol attachment are similar but not identical in mammalian cells and parasitic protozoa.

1994 • 116 citations

Essential Roles for GPI-anchored Proteins in African Trypanosomes Revealed Using Mutants Deficient in GPI8

2003 • 115 citations

Selectivity of the cleavage/attachment site of phosphatidylinositol-glycan-anchored membrane proteins determined by site-specific mutagenesis at Asp-484 of placental alkaline phosphatase.

1990 • 114 citations

Leishmania promastigotes require lipophosphoglycan to actively modulate the fusion properties of phagosomes at an early step of phagocytosis

2000 • 114 citations

The GPI Transamidase Complex ofSaccharomyces cerevisiaeContains Gaa1p, Gpi8p, and Gpi16p

2001 • 112 citations

Delineation of Three Pathways of Glycosylphosphatidylinositol Biosynthesis in Leishmania mexicana

1998 • 108 citations

Ether Phospholipids and Glycosylinositolphospholipids Are Not Required for Amastigote Virulence or for Inhibition of Macrophage Activation by Leishmania major

2003 • 106 citations

Anchoring of membrane proteins via phosphatidylinositol is deficient in two classes of Thy-1 negative mutant lymphoma cells.

1986 • 105 citations

Specific proteins are required to translocate phosphatidylcholine bidirectionally across the endoplasmic reticulum

2000 • 100 citations

Signaling of immune system cells by glycosylphosphatidylinositol (GPI) anchor and related structures derived from parasitic protozoa

2000 • 100 citations

Stimulation of the Hydrolytic Activity and Decrease of the Transpeptidase Activity of γ-Glutamyl Transpeptidase by Maleate; Identity of a Rat Kidney Maleate-Stimulated Glutaminase and γ-Glutamyl Transpeptidase

1974 • 90 citations

Active Site Determination of Gpi8p, a Caspase-Related Enzyme Required for Glycosylphosphatidylinositol Anchor Addition to Proteins

2000 • 89 citations

Evidence that free GPI glycolipids are essential for growth of Leishmania mexicana

1999 • 87 citations

Phosphoglycan Repeat-deficient Leishmania mexicana Parasites Remain Infectious to Macrophages and Mice

2001 • 86 citations

A ricin agglutinin-resistant clone of Leishmania donovani deficient in lipophosphoglycan

1988 • 86 citations

Identification of six complementation classes involved in the biosynthesis of glycosylphosphatidylinositol anchors in Saccharomyces cerevisiae.

1995 • 86 citations

[42] Legumain: Asparaginyl endopeptidase

1994 • 84 citations

Two Subunits of Glycosylphosphatidylinositol Transamidase, GPI8 and PIG-T, Form a Functionally Important Intermolecular Disulfide Bridge

2003 • 82 citations

A Putative Vacuolar Processing Protease Is Regulated by Ethylene and also during Fruit Ripening in Citrus Fruit

1995 • 81 citations

Transfer of glycosyl-phosphatidylinositol membrane anchors to polypeptide acceptors in a cell-free system.

1991 • 80 citations

Leishmania mexicanaMutants Lacking Glycosylphosphatidylinositol (GPI):Protein Transamidase Provide Insights into the Biosynthesis and Functions of GPI-anchored Proteins

2000 • 80 citations

The Role of Phosphomannose Isomerase in Leishmania mexicana Glycoconjugate Synthesis and Virulence

2001 • 80 citations

An Active Carbonyl Formed during Glycosylphosphatidylinositol Addition to a Protein Is Evidence of Catalysis by a Transamidase

1995 • 79 citations

Derivation and characterization of glycoinositol-phospholipid anchor-defective human K562 cell clones.

1992 • 77 citations

The affected gene underlying the class K glycosylphosphatidylinositol (GPI) surface protein defect codes for the GPI transamidase

1997 • 77 citations

The yeast spt14 gene is homologous to the human PIG-A gene and is required for GPI anchor synthesis.

1995 • 69 citations

Identification of a Compensatory Mutant ( lpg2 ⁻ REV ) of Leishmania major Able To Survive as Amastigotes within Macrophages without LPG2 -Dependent Glycoconjugates and Its Significance to Virulence and Immunization Strategies

2004 • 68 citations

New fluorescent probes reveal that flippase-mediated flip-flop of phosphatidylinositol across the endoplasmic reticulum membrane does not depend on the stereochemistry of the lipid

2005 • 66 citations

What Can GPI Do for You?

1994 • 66 citations

A glycosylphosphatidylinositol (GPI)-negative phenotype produced in Leishmania major by GPI phospholipase C from Trypanosoma brucei: topography of two GPI pathways

1994 • 65 citations

Segregation of Glycosylphosphatidylinositol Biosynthetic Reactions in a Subcompartment of the Endoplasmic Reticulum

1999 • 65 citations

A Cell-free Assay for Glycosylphosphatidylinositol Anchoring in African Trypanosomes

1999 • 63 citations

Asparaginyl endopeptidase activity in adultSchistosoma mansoni

1995 • 63 citations

Isolation and Analysis of cDNA Encoding a Precursor of Canavalia ensiformis Asparaginyl Endopeptidase (Legumain)

1994 • 62 citations

Fusion of sequence elements from non-anchored proteins to generate a fully functional signal for glycophosphatidylinositol membrane anchor attachment.

1991 • 62 citations

No glycolipid anchors are added to Thy-1 glycoprotein in Thy-1-negative mutant thymoma cells of four different complementation classes.

1988 • 60 citations

Glycoinositol phospholipid anchor-defective K562 mutants with biochemical lesions distinct from those in Thy-1- murine lymphoma mutants.

1994 • 59 citations

Regulation of host cell function by glycosylphosphatidylinositols of the parasitic protozoa

1996 • 59 citations

Biosynthesis of phosphatidylinositol glycan-anchored membrane proteins.

1991 • 59 citations

Lipophosphoglycan expression and virulence in Ricin-resistant variants of Leishmania major

1990 • 59 citations

Flip-flop of glycosylphosphatidylinositols (GPI's) across the ER

2004 • 58 citations

Evidence for a role for the Dictyostelium Rap1 in cell viability and the response to osmotic stress

2002 • 57 citations

GPI transamidase of Trypanosoma brucei has two previously uncharacterized (trypanosomatid transamidase 1 and 2) and three common subunits

2003 • 54 citations

Molecular Genetics of Paroxysmal Nocturnal Hemoglobinuria

2003 • 52 citations

Mutational analysis of the variant surface glycoprotein GPI-anchor signal sequence inTrypanosoma brucei

2002 • 51 citations

Molecular cloning of human homolog of yeast GAA1 which is required for attachment of glycosylphosphatidylinositols to proteins¹

1998 • 51 citations

Structural Requirements for the Recruitment of Gaa1 into a Functional Glycosylphosphatidylinositol Transamidase Complex

2002 • 50 citations

Identification of SPT14/CWH6 as the yeast homologue of hPIG-A, a gene involved in the biosynthesis of GPI anchors

1995 • 48 citations

Selectivity at the cleavage/attachment site of phosphatidylinositol-glycan anchored membrane proteins is enzymatically determined.

1990 • 47 citations

Processing and Trafficking of Leishmania mexicanaGP63

2002 • 41 citations

Evidence that the putative COOH-terminal signal transamidase involved in glycosylphosphatidylinositol protein synthesis is present in the endoplasmic reticulum.

1993 • 40 citations

Phosphatidylinositol Hydrolysis by Glycosylphosphatidylinositol Phospholipase C

1996 • 39 citations

Endoplasmic reticulum proteins involved in glycosylphosphatidylinositol‐anchor attachment

2001 • 38 citations

Early Events in Glycosylphosphatidylinositol Anchor Addition

2001 • 38 citations

GPI anchor transamidase ofTrypanosoma brucei: in vitro assay of the recombinant protein and VSG anchor exchange

2002 • 38 citations

Glycobiology of Plasmodium falciparum

2001 • 37 citations

A defect in glycosylphosphatidylinositol (GPI) transamidase activity in mutant K cells is responsible for their inability to display GPI surface proteins.

1996 • 37 citations

Nuclear inositol lipid metabolism: More than just second messenger generation?

2005 • 36 citations

A Conserved Proline in the Last Transmembrane Segment of Gaa1 Is Required for Glycosylphosphatidylinositol (GPI) Recognition by GPI Transamidase

2004 • 35 citations

Nuclear phosphoinositide specific phospholipase C (PI-PLC)-beta 1: a central intermediary in nuclear lipid-dependent signal transduction.

2005 • 35 citations

COOH-terminal processing of nascent polypeptides by the glycosylphosphatidylinositol transamidase in the presence of hydrazine is governed by the same parameters as glycosylphosphatidylinositol addition.

1996 • 34 citations

Soluble GPI8 restores glycosylphosphatidylinositol anchoring in a trypanosome cell-free system depleted of lumenal endoplasmic reticulum proteins

2000 • 33 citations

Roles of free GPIs in amastigotes of Leishmania

1999 • 32 citations

Enzymic hydrolysis and synthesis of peptide bonds.

1955 • 32 citations

Mutational analysis of the C-terminal signal peptide of bovine liver 5′-nucleotidase for GPI anchoring: a study on the significance of the hydrophilic spacer region

1997 • 32 citations

Biosynthesis of phosphatidylinositol-glycan (PI-G)-anchored membrane proteins in cell-free systems: cleavage of the nascent protein and addition of the PI-G moiety depend on the size of the COOH-terminal signal peptide.

1992 • 32 citations

Ke 6 Gene

1995 • 30 citations

Recognition of the carboxyl-terminal signal for GPI modification requires translocation of its hydrophobic domain across the ER membrane 1 1Edited by I. B. Holland

1999 • 28 citations

Glycosylphosphatidylinositols are required for the development of Trypanosoma cruzi amastigotes

1997 • 27 citations

Gpi17p does not stably interact with other subunits of glycosylphosphatidylinositol transamidase in Saccharomyces cerevisiae

2005 • 24 citations

Glycosyl-phosphatidylinositol anchor attachment in a yeast in vitro system

1997 • 23 citations

[39] Processing of nascent proteins to glycosylphosphatidylinositol-anchored forms in cell-free systems

1995 • 20 citations

Ricin-resistant mutants ofLeishmania majorwhich express modified lipophosphoglycan remain infective for mice

1994 • 20 citations

GPI-anchor deficiency in myeloid cells causes impaired FcγR effector functions

2004 • 20 citations

Characterization of a Leishmania mexicana mutant defective in synthesis of free and protein-linked GPI glycolipids

2002 • 16 citations

Trypanosomatid Genomes

2005 • 15 citations

Biosynthesis of glycophosphoinositol anchors in

1991 • 12 citations

Enhanced Responses of Glycosylphosphatidylinositol Anchor-Deficient T Lymphocytes

2004 • 11 citations

Cloning of murine glycosyl phosphatidylinositol anchor attachment protein, GPAA1

2000 • 7 citations

The requirements for GPI-attachment are similar but not identical in mammalian cells and parasitic protozoa.

1994 • 4 citations

Free and protein-linked glycoinositolphospholipids in Trypanosoma cruzi.

1994 • 2 citations