Targeted gene deletion of Leishmania major genes encoding developmental stage‐specific leishmanolysin (GP63)

Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae

1983 • 810 citations

THE LIPOPHOSPHOGLYCAN OF LEISHMANIA PARASITES

1992 • 473 citations

Stable transfection of the human parasite Leishmania major delineates a 30-kilobase region sufficient for extrachromosomal replication and expression.

1990 • 450 citations

Double targeted gene replacement for creating null mutants.

1991 • 323 citations

Role of the Leishmania surface protease gp63 in complement fixation, cell adhesion, and resistance to complement-mediated lysis.

1995 • 309 citations

Rapid isolation of DNA from trypanosomatid protozoa using a simple ‘mini-prep’ procedure

1993 • 275 citations

Stage-Specific Adhesion of Leishmania Promastigotes to the Sandfly Midgut

1992 • 245 citations

The Leishmania receptor for macrophages is a lipid-containing glycoconjugate.

1985 • 235 citations

Structure of the lipophosphoglycan from Leishmania major.

1990 • 222 citations

Evidence that the vectorial competence of phlebotomine sand flies for different species of Leishmania is controlled by structural polymorphisms in the surface lipophosphoglycan.

1994 • 208 citations

The major surface protein of Leishmania promastigotes is a protease.

1986 • 207 citations

Complement binding by two developmental stages of Leishmania major promastigotes varying in expression of a surface lipophosphoglycan.

1988 • 202 citations

Stage-specific binding of Leishmania donovani to the sand fly vector midgut is regulated by conformational changes in the abundant surface lipophosphoglycan.

1995 • 179 citations

Molecular cloning of the major surface antigen of leishmania.

1988 • 169 citations

The promastigote surface protease (gp63) of Leishmania is expressed but differentially processed and localized in the amastigote stage

1989 • 159 citations

The generation of infective stage Leishmania major promastigotes is associated with the cell-surface expression and release of a developmentally regulated glycolipid.

1987 • 141 citations

Genes encoding the major surface glycoprotein in Leishmania are tandemly linked at a single chromosomal locus and are constitutively transcribed

1989 • 131 citations

Three distinct RNAs for the surface protease gp63 are differentially expressed during development of Leishmania donovani chagasi promastigotes to an infectious form.

1992 • 129 citations

Acid protease activity of a major surface membrane glycoprotein (gp63) from Leishmania mexicana promastigotes

1988 • 124 citations

Growth cycle-dependent generation of complement-resistant Leishmania promastigotes.

1985 • 122 citations

Developmental modification of the lipophosphoglycan from Leishmania major promastigotes during metacyclogenesis

1990 • 120 citations

Deletion of an immunodominant Trypanosoma cruzi surface glycoprotein disrupts flagellum-cell adhesion

1993 • 115 citations

The macrophage-attachment glycoprotein gp63 is the predominant C3-acceptor site on Leishmania mexicana promastigotes

1987 • 110 citations

Characterization of the promastigote surface protease of Leishmania as a membrane-bound zinc endopeptidase

1989 • 95 citations

Ser/Thr-rich repetitive motifs as targets for phosphoglycan modifications in Leishmania mexicana secreted acid phosphatase.

1995 • 87 citations

The major surface glycoprotein (GP63) is present in both life stages of Leishmania

1990 • 86 citations

Cell surface lipophosphoglycan of Leishmania donovani

1987 • 77 citations

Structually distinct genes for the surface protease of Leishmania mexicana are developmentally regulated

1993 • 76 citations

Identification of a surface metalloproteinase on 13 species of Leishmania isolated from humans, Crithidia fasciculata, and Herpetomonas samuelpessoai

1992 • 66 citations

Null mutants for the lmcpa cysteine proteinase gene in Leishmania mexicana

1994 • 63 citations

Differentially expressed Leishmania major gp63 genes encode cell surface leishmanolysin with distinct signals for glycosylphosphatidylinositol attachment1Note: Nucleotide sequence data reported in this paper are available in the GenBank™ database under the accession number AF039721.1

1998 • 62 citations

Leishmania major and L. donovani: Effects on proteolytic enzymes of Phlebotomus papatasi (Diptera, Psychodidae)

1986 • 54 citations

Crithidia fasciculata contains a transcribed leishmanial surface proteinase (gp63) gene homologue

1993 • 53 citations

Genetic rescue of surface metalloproteinase (gp63)-deficiency in Leishmania amazonensis variants increases their infection of macrophages at the early phase

1994 • 53 citations

The gene encoding streptothricin acetyltransferase (sat) as a selectable marker for Leishmania expression vectors

1995 • 52 citations

Analysis of the active site and activation mechanism of the Leishmania surface metalloproteinase GP63

1995 • 50 citations

Identification of Leishmania genes encoding proteins containing tandemly repeating peptides.

1987 • 48 citations

Modification of GP63 genes from diverse species of Leishmania for expression of recombinant protein at high levels in Escherichia coli

1991 • 39 citations

Released glycoconjugate of indigenous Leishmania major enhances survival of a foreign L. major in Phlebotomus papatasi

1990 • 38 citations

Recombinant Leishmania surface glycoprotein GP63 is secreted in the baculovirus expression system as a latent metalloproteinase

1993 • 31 citations

Mutational and functional analysis of theLeishmaniasurface metalloproteinase GP63: similarities to matrix metalloproteinases

1994 • 30 citations

The nucleotide sequence ofleuAfromSalmonella typhimurium

1990 • 27 citations

Nucleotide sequence of a plasmid born streptothricin-acetyl-transferase gene (sat-1)

1989 • 22 citations

Leishmania major HEXBP deletion mutants generated by double targeted gene replacement

1994 • 20 citations

Nucleotide sequence of the streptothricin-acetyl-transferase gene sat-2

1990 • 14 citations