A Cell-free Assay for Glycosylphosphatidylinositol Anchoring in African Trypanosomes

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A major 125-kd membrane glycoprotein of Saccharomyces cerevisiae is attached to the lipid bilayer through an inositol-containing phospholipid.

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The affected gene underlying the class K glycosylphosphatidylinositol (GPI) surface protein defect codes for the GPI transamidase

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Developmental variation of glycosylphosphatidylinositol membrane anchors in Trypanosoma brucei. In vitro biosynthesis of intermediates in the construction of the GPI anchor of the major procyclic surface glycoprotein.

1992 • 50 citations

[46] Purification and use of recombinant glycosylphosphatidylinositol-phospholipase C

1995 • 42 citations

Cleavage without anchor addition accompanies the processing of a nascent protein to its glycosylphosphatidylinositol-anchored form.

1995 • 42 citations

Evidence that the putative COOH-terminal signal transamidase involved in glycosylphosphatidylinositol protein synthesis is present in the endoplasmic reticulum.

1993 • 40 citations

The translocon: more than a hole in the ER membrane?

1996 • 37 citations

Phosphatidylinositol-glycan (PI-G)-anchored membrane proteins: requirement of ATP and GTP for translation-independent COOH-terminal processing.

1992 • 29 citations

Soluble constituents of the ER lumen are required for GPI anchoring of a model protein.

1995 • 26 citations

Glycosyl-phosphatidylinositol anchor attachment in a yeast in vitro system

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A cleavable N-terminal signal peptide is not a prerequisite for the biosynthesis of glycosylphosphatidylinositol-anchored proteins.

1994 • 20 citations

[39] Processing of nascent proteins to glycosylphosphatidylinositol-anchored forms in cell-free systems

1995 • 20 citations