Reporter epitopes: A novel approach to examine transmembrane topology of integral membrane proteins applied to the .alpha.1 subunit of the nicotinic acetylcholine receptor

Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter

1984 • 6,459 citations

Nicotinic Acetylcholine Receptor an 9 Å Resolution

1993 • 822 citations

Structural homology of Torpedo californica acetylcholine receptor subunits

1983 • 798 citations

Acetylcholine Receptor Channel Structure Probed in Cysteine-Substitution Mutants

1992 • 668 citations

A Genetic Approach to Analyzing Membrane Protein Topology

1986 • 610 citations

Molecular Neurobiology of Glutamate Receptors

1992 • 436 citations

Nucleotide and deduced amino acid sequences of Torpedo californica acetylcholine receptor gamma subunit.

1983 • 412 citations

Complete mRNA coding sequence of the acetylcholine binding alpha-subunit of Torpedo marmorata acetylcholine receptor: a model for the transmembrane organization of the polypeptide chain.

1983 • 332 citations

Determinants of membrane protein topology.

1987 • 301 citations

[29] Production and assay of antibodies to acetylcholine receptors

1981 • 277 citations

Rapid insertional mutagenesis of DNA by polymerase chain reaction (PCR)

1989 • 218 citations

A muscle acetylcholine receptor is expressed in the human cerebellar medulloblastoma cell line TE671

1989 • 186 citations

Transmembrane topography of the nicotinic acetylcholine receptor: immunochemical tests contadict theoretical predictions based on hydrophobicity profiles

1986 • 183 citations

Genetic analysis of the membrane insertion and topology of MalF, a cytoplasmic membrane protein of Escherichia coli

1988 • 175 citations

Localization and membrane topology of EnvZ, a protein involved in osmoregulation of OmpF and OmpC in Escherichia coli.

1987 • 174 citations

TOPOGRAPHY OF MEMBRANE PROTEINS

1989 • 155 citations

Characteristics of monoclonal antibodies to denatured Torpedo and to native calf acetylcholine receptors: Species, subunit and region specificity

1986 • 127 citations

Explorations of the nicotinic acetylcholine receptor.

1989 • 113 citations

The human medulloblastoma cell line TE671 expresses a muscle‐like acetylcholine receptor Cloning of the α‐subunit cDNA

1988 • 110 citations

Evidence for unpredicted transmembrane domains in acetylcholine receptor subunits.

1985 • 103 citations

Topological mapping of acetylcholine receptor: evidence for a model with five transmembrane segments and a cytoplasmic COOH-terminal peptide.

1985 • 101 citations

Expression of fusion proteins of the nicotinic acetylcholine receptor from mammalian muscle identifies the membrane-spanning regions in the alpha and delta subunits.

1992 • 88 citations

The transmembrane topology of the amino terminus of the alpha subunit of the nicotinic acetylcholine receptor

1991 • 84 citations

The sidedness of the COOH terminus of the acetylcholine receptor δ subunit

1989 • 69 citations

Determination of amino acids critical to the main immunogenic region of intact acetylcholine receptors by in vitro mutagenesis

1990 • 64 citations

Immunochemical demonstration that amino acids 360-377 of the acetylcholine receptor gamma-subunit are cytoplasmic.

1985 • 58 citations

TE671 cells express an abundance of a partially mature acetylcholine receptor alpha subunit which has characteristics of an assembly intermediate.

1990 • 53 citations

Structural features of the nicotinic acetylcholine receptor revealed by antibodies to synthetic peptides

1984 • 53 citations

Transmembrane topography of the nicotinic acetylcholine receptor delta subunit.

1987 • 52 citations

Epitope mapping of antibodies to acetylcholine receptor .alpha. subunits using peptides synthesized on polypropylene pegs

1991 • 41 citations

The main immunogenic region of the nicotinic acetylcholine receptor: Interaction of monoclonal antibodies with synthetic peptides

1989 • 34 citations

Identification of a cytoplasmic region of the Torpedo nicotinic acetylcholine receptor alpha-subunit by epitope mapping.

1990 • 32 citations

Proteolytic fragments of the nicotinic acetylcholine receptor identified by mass spectrometry: implications for receptor topography

1989 • 31 citations

Monoclonal antibodies against synthetic sequences of the nicotinic receptor cross-react fully with the native receptor and reveal the transmembrane disposition of their epitopes

1993 • 12 citations