A strategy for isolation of cDNAs encoding proteins affecting human intestinal epithelial cell growth and differentiation: characterization of a novel gut-specific N-myristoylated annexin.

A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity

1983 • 26,063 citations

Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease

1979 • 22,482 citations

Improved tools for biological sequence comparison.

1988 • 11,455 citations

Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

1980 • 8,711 citations

An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs

1987 • 5,139 citations

Stem cells: attributes, cycles, spirals, pitfalls and uncertainties. Lessons for and from the crypt

1990 • 1,793 citations

Origin, differentiation and renewal of the four main epithelial cell types in the mouse small intestine V. Unitarian theory of the origin of the four epithelial cell types

1974 • 1,697 citations

One Hundred and Twenty-Seven Cultured Human Tumor Cell Lines Producing Tumors in Nude Mice23

1977 • 1,556 citations

Classification of human colorectal adenocarcinoma cell lines.

1976 • 943 citations

THE BIOLOGY AND ENZYMOLOGY OF EUKARYOTIC PROTEIN ACYLATION

1988 • 787 citations

Cloning and expression of human lipocortin, a phospholipase A2 inhibitor with potential anti-inflammatory activity

1986 • 671 citations

A solvent system for delipidation of plasma or serum without protein precipitation

1976 • 515 citations

Expression and intracellular transport of microvillus membrane hydrolases in human intestinal epithelial cells.

1985 • 497 citations

Epithelial polarity, villin expression, and enterocytic differentiation of cultured human colon carcinoma cells: a survey of twenty cell lines.

1988 • 458 citations

A short sequence in the p60src N terminus is required for p60src myristylation and membrane association and for cell transformation.

1984 • 447 citations

Aggregation of chromaffin granules by calpactin at micromolar levels of calcium

1988 • 443 citations

Two human 35 kd inhibitors of phospholipase A2 are related to substrates of pp60v-src and of the epidermal growth factor receptor/kinase

1986 • 421 citations

Identification of a cellular protein substrate phosphorylated by the avian sarcoma virus-transforming gene product

1980 • 420 citations

Growth adaptation to methotrexate of HT-29 human colon carcinoma cells is associated with their ability to differentiate into columnar absorptive and mucus-secreting cells.

1990 • 414 citations

Identification and purification of an adrenal medullary protein (synexin) that causes calcium-dependent aggregation of isolated chromaffin granules.

1978 • 396 citations

How the messenger got its tail: addition of poly(A) in the nucleus

1990 • 390 citations

Mutation of NH2-terminal glycine of p60src prevents both myristoylation and morphological transformation.

1985 • 386 citations

Diversity in the lipocortin/calpactin family

1988 • 376 citations

Characterization by Tandem Mass Spectrometry of Structural Modifications in Proteins

1987 • 367 citations

Five distinct calcium and phospholipid binding proteins share homology with lipocortin I.

1988 • 364 citations

Absorptive and mucus-secreting subclones isolated from a multipotent intestinal cell line (HT-29) provide new models for cell polarity and terminal differentiation.

1987 • 343 citations

Derivation of mouse intestinal crypts from single progenitor cells

1985 • 330 citations

A role for calpactin in calcium-dependent exocytosis in adrenal chromaffin cells

1989 • 321 citations

The cDNA sequence for the protein-tyrosine kinase substrate p36 (calpactin I heavy chain) reveals a multidomain protein with internal repeats

1986 • 297 citations

Protein N-myristoylation

1991 • 289 citations

G-protein alpha-subunit expression, myristoylation, and membrane association in COS cells.

1990 • 277 citations

Differentiation requires continuous regulation.

1991 • 276 citations

Lipid modifications of G protein subunits.

1991 • 274 citations

cis-Unsaturated fatty acids induce the fusion of chromaffin granules aggregated by synexin.

1981 • 272 citations

A clonal marker induced by mutation in mouse intestinal epithelium

1988 • 255 citations

Developmental regulation of cryptdin, a corticostatin/defensin precursor mRNA in mouse small intestinal crypt epithelium.

1989 • 254 citations

Protein N-myristoylation in Escherichia coli: reconstitution of a eukaryotic protein modification in bacteria.

1990 • 245 citations

Characterization of Ca2+-dependent phospholipid binding and phosphorylation of lipocortin I.

1987 • 236 citations

Development of the pattern of cell renewal in the crypt-villus unit of chimaeric mouse small intestine

1988 • 235 citations

Amino-terminal processing of proteins by N-myristoylation. Substrate specificity of N-myristoyl transferase.

1987 • 220 citations

A simple subtractive hybridization technique employing photoactivatable biotin and phenol extraction

1988 • 219 citations

Purification and partial sequence analysis of a 37-kDa protein that inhibits phospholipase A2 activity from rat peritoneal exudates.

1986 • 204 citations

A calcium-dependent 35-kilodalton substrate for epidermal growth factor receptor/kinase isolated from normal tissue.

1986 • 203 citations

Fatty acylated proteins as components of intracellular signaling pathways

1990 • 202 citations

Cell migration pathway in the intestinal epithelium: An in situ marker system using mouse aggregation chimeras

1985 • 187 citations

Myristoyl CoA:protein N-myristoyltransferase activities from rat liver and yeast possess overlapping yet distinct peptide substrate specificities.

1988 • 187 citations

Regulation of calpactin I phospholipid binding by calpactin I light-chain binding and phosphorylation by p60v-src

1987 • 184 citations

Identification of chromaffin granule-binding proteins. Relationship of the chromobindins to calelectrin, synhibin, and the tyrosine kinase substrates p35 and p36.

1987 • 175 citations

The absence of myristic acid decreases membrane binding of p60src but does not affect tyrosine protein kinase activity

1986 • 174 citations

Stem-cell organization in mouse small intestine

1990 • 170 citations

Demonstration of somatic mutation and colonic crypt clonality by X-linked enzyme histochemistry

1988 • 170 citations

The intracellular accumulation of UDP-N-acetylhexosamines is concomitant with the inability of human colon cancer cells to differentiate.

1985 • 168 citations

Cell-type-specific cDNA probes and the murine I region: the localization and orientation of Ad alpha.

1984 • 167 citations

Identification of a 32K plasma membrane protein that binds to the myristylated amino-terminal sequence of p60v-src

1990 • 162 citations

Mechanisms underlying generation of gradients in gene expression within the intestine: an analysis using transgenic mice containing fatty acid binding protein-human growth hormone fusion genes.

1988 • 161 citations

George Otto Gey. (1899-1970). The HeLa cell and a reappraisal of its origin.

1971 • 160 citations

Calcium channel activity of purified human synexin and structure of the human synexin gene.

1989 • 153 citations

Epidermal growth factor stimulates the phosphorylation of the calcium-dependent 35,000-dalton substrate in intact A-431 cells.

1985 • 149 citations

A Short Sequence in the p60^src N Terminus Is Required for p60^src Myristylation and Membrane Association and for Cell Transformation

1984 • 149 citations

Specific and saturable binding of pp60v-src to plasma membranes: Evidence for a myristyl-src receptor

1989 • 137 citations

The amino acid sequence of protein II and its phosphorylation site for protein kinase C; the domain structure Ca2+-modulated lipid binding proteins.

1987 • 137 citations

Differential Tissue Expression of Three 35-kDa Annexin Calcium-dependent Phospholipid-binding Proteins

1989 • 136 citations

Specialized cell types in the human fetal small intestine

1978 • 134 citations

Primary structure of human placental anticoagulant protein

1987 • 132 citations

Ca2+-activated synexin forms highly selective, voltage-gated Ca2+ channels in phosphatidylserine bilayer membranes.

1988 • 124 citations

Protein fatty acid acylation: enzymatic synthesis of an N-myristoylglycyl peptide.

1986 • 123 citations

Spatial differentiation of the intestinal epithelium: analysis of enteroendocrine cells containing immunoreactive serotonin, secretin, and substance P in normal and transgenic mice.

1990 • 123 citations

Mapping enteroendocrine cell populations in transgenic mice reveals an unexpected degree of complexity in cellular differentiation within the gastrointestinal tract.

1990 • 115 citations

Role of synexin in membrane fusion. Enhancement of calcium-dependent fusion of phospholipid vesicles.

1981 • 114 citations

Posttranslational Regulation of Sucrase-Isomaltase Expression in Intestinal Crypt and Villus Cells

1989 • 114 citations

The continuous growth of vertebrate cells in the absence of sugar.

1981 • 114 citations

Three Ca2+-binding proteins from porcine liver and intestine differ immunologically and physicochemically and are distinct in Ca2+ affinities.

1985 • 111 citations

Development of the intestinal disaccharidase and alkaline phosphatase activities in the human foetus.

1966 • 108 citations

The 46,000-dalton tyrosine protein kinase substrate is widespread, whereas the 36,000-dalton substrate is only expressed at high levels in certain rodent tissues.

1984 • 103 citations

Identity of Inositol 1,2-Cyclic Phosphate 2-Phosphohydrolase with Lipocortin III

1990 • 102 citations

Role of lipocortin I in the glucocorticoid induction of the terminal differentiation of a human squamous carcinoma

1990 • 99 citations

Association of the S-100-related calpactin I light chain with the NH2-terminal tail of the 36-kDa heavy chain.

1986 • 98 citations

Human intestinal goblet cells in monolayer culture: Characterization of a mucus-secreting subclone derived from the HT29 colon adenocarcinoma cell line

1988 • 98 citations

The protein-tyrosine kinase substrate, calpactin I heavy chain (p36), is part of the primer recognition protein complex that interacts with DNA polymerase alpha.

1991 • 95 citations

The substrate specificity of Saccharomyces cerevisiae myristoyl-CoA:protein N-myristoyltransferase. Analysis of myristic acid analogs containing oxygen, sulfur, double bonds, triple bonds, and/or an aromatic residue

1991 • 94 citations

Human liver fatty acid binding protein. Isolation of a full length cDNA and comparative sequence analyses of orthologous and paralogous proteins.

1985 • 87 citations

Lipocortin I (p35) is abundant in a restricted number of differentiated cell types in adult organs

1989 • 87 citations

Development of villus absorptive cells in the human fetal small intestine: A morphological and morphometric study

1979 • 83 citations

Heteroatom-substituted fatty acid analogs as substrates for N-myristoyltransferase: an approach for studying both the enzymology and function of protein acylation.

1988 • 80 citations

Incorporation of 12-methoxydodecanoate into the human immunodeficiency virus 1 gag polyprotein precursor inhibits its proteolytic processing and virus production in a chronically infected human lymphoid cell line.

1991 • 70 citations

Changes in the distribution of the 34-kdalton tyrosine kinase substrate during differentiation and maturation of chicken tissues.

1984 • 69 citations

Structural and functional studies of Saccharomyces cerevisiae myristoyl-CoA:protein N-myristoyltransferase produced in Escherichia coli. Evidence for an acyl-enzyme intermediate.

1990 • 68 citations

Analyzing the substrate specificity of Saccharomyces cerevisiae myristoyl-CoA:protein N-myristoyltransferase by co-expressing it with mammalian G protein alpha subunits in Escherichia coli

1991 • 67 citations

Use of transgenic mice to infer the biological properties of small intestinal stem cells and to examine the lineage relationships of their descendants.

1991 • 66 citations

Functional identity of a primer recognition protein as phosphoglycerate kinase.

1990 • 59 citations

Development of Brush Border Peptidases in Human and Rat Small Intestine during Fetal and Neonatal Life

1981 • 57 citations

Differentiation of HL‐60 cells is associated with an increase in the 35‐kDa protein lipocortin I

1988 • 54 citations

Evaluation of the annexins as potential mediators of membrane fusion in exocytosis

1990 • 53 citations

Clonal analysis of intestinal crypt populations in mouse aggregation chimaeras

1985 • 44 citations

Altered membrane association of p60v-src and a murine 63-kDa N-myristoyl protein after incorporation of an oxygen-substituted analog of myristic acid.

1989 • 44 citations

A transgenic mouse model that is useful for analyzing cellular and geographic differentiation of the intestine during fetal development

1989 • 42 citations

Epithelial cell differentiation in normal and transgenic mouse intestinal isografts.

1991 • 41 citations

Expression of liver fatty acid-binding protein/human growth hormone fusion genes within the enterocyte and enteroendocrine cell populations of fetal transgenic mice.

1991 • 35 citations

Functional analysis of protein N-myristoylation: metabolic labeling studies using three oxygen-substituted analogs of myristic acid and cultured mammalian cells provide evidence for protein-sequence-specific incorporation and analog-specific redistribution.

1990 • 35 citations

Immunohistochemical distribution of hepatic fatty acid-binding protein in rat and human alimentary tract.

1988 • 31 citations

Effect of gamma radiation at high- and low-dose rate on a novel in vivo mutation assay in mouse intestine

1989 • 30 citations

Lipocortin IV is a basolateral cytoskeleton constituent of rabbit enterocytes.

1991 • 23 citations

Cyclic hydrolase-transfected 3T3 cells have low levels of inositol 1,2-cyclic phosphate and reach confluence at low density

1991 • 21 citations

Ethylnitrosourea-induced mutations in vivo involving the Dolichos biflorus agglutinin receptor in mouse intestinal epithelium

1990 • 20 citations