The AU-rich sequences present in the introns of plant nuclear pre-mRNAs are required for splicing

Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

1987 • 64,270 citations

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

1987 • 45,673 citations

Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter

1984 • 6,459 citations

Optimal computer folding of large RNA sequences using thermodynamics and auxiliary information

1981 • 3,596 citations

Improved free-energy parameters for predictions of RNA duplex stability.

1986 • 1,472 citations

SPLICING OF MESSENGER RNA PRECURSORS

1986 • 1,233 citations

Splicing of Messenger RNA Precursors

1987 • 919 citations

Splicing of Messenger RNA Precursors

1984 • 874 citations

A compensatory base change in U1 snRNA suppresses a 5′ splice site mutation

1986 • 755 citations

PRE-mRNA SPLICING

1986 • 673 citations

Direct gene transfer to plants

1984 • 586 citations

A survey on intron and exon lengths

1988 • 569 citations

U2 as well as U1 small nuclear ribonucleoproteins are involved in premessenger RNA splicing

1985 • 568 citations

A factor, U2AF, is required for U2 snRNP binding and splicing complex assembly

1988 • 535 citations

Stepwise assembly of a pre-mRNA splicing complex requires U-snRNPs and specific intron sequences

1985 • 524 citations

Recognition of the TACTAAC box during mRNA splicing in yeast involves base pairing to the U2-like snRNA

1987 • 508 citations

SPLICEOSOMAL snRNAs

1988 • 463 citations

Gene Structure in Eukaryotic Microbes

1987 • 462 citations

A catalogue of splice junction and putative branch point sequences from plant introns

1986 • 376 citations

Yeast mRNA splicing in vitro.

1985 • 337 citations

A minimal intron length but no specific internal sequence is required for splicing the large rabbit β-globin intron

1984 • 328 citations

The 3′ Splice Site of Pre-Messenger RNA Is Recognized by a Small Nuclear Ribonucleoprotein

1985 • 310 citations

The expression of a nopaline synthase ? human growth hormone chimaeric gene in transformed tobacco and sunflower callus tissue

1986 • 301 citations

Expression in plants of two bacterial antibiotic resistance genes after protoplast transformation with a new plant expression vector

1986 • 299 citations

Functions of the Abundant U-snRNPs

1988 • 284 citations

A protein associated with small nuclear ribonucleoprotein particles recognizes the 3′ splice site of premessenger RNA

1986 • 283 citations

Effects of RNA secondary structure on alternative splicing of Pre-mRNA: Is folding limited to a region behind the transcribing RNA polymerase?

1988 • 280 citations

A protein that specifically recognizes the 3′ splice site of mammalian pre-mRNA introns is associated with a small nuclear ribonucleoprotein

1986 • 274 citations

RNA binding specificity of hnRNP proteins: a subset bind to the 3′ end of introns.

1988 • 251 citations

Cryptic branch point activation allows accurate in vitro splicing of human β-globin intron mutants

1985 • 236 citations

Complete nucleotide sequence of a French bean storage protein gene: Phaseolin

1983 • 218 citations

mRNA splicing efficiency in yeast and the contribution of nonconserved sequences

1985 • 194 citations

Monocot and dicot pre-mRNAs are processed with different efficiencies in transgenic tobacco

1986 • 192 citations

Role of the 3′ splice site consensus sequence in mammalian pre-mRNA splicing

1985 • 184 citations

Cis and trans mRNA splicing in C. elegans

1988 • 144 citations

Amount of RNA secondary structure required to induce an alternative splice.

1987 • 128 citations

Cleavage of 5′ splice site and lariat formation are independent of 3′ splice site in yeast mRNA splicing

1985 • 122 citations

Transcription and splicing

1989 • 120 citations

Factors influencing alternative splice site utilization in vivo.

1987 • 120 citations

Soybean leghemoglobin gene family: normal, pseudo, and truncated genes.

1982 • 116 citations

Effect of mutations at the lariat branch acceptor site on β-globin pre-mRNA splicing in vitro

1986 • 111 citations

Abnormal expression of chromosomal rabbit β-globin gene in Saccharomyces cerevisiae

1980 • 110 citations

Phenotypic Diversity Mediated by the Maize Transposable Elements Ac and Spm

1988 • 110 citations

Nuclear Pre-mRNA Processing in Plants: Distinct Modes of 3′-Splice-Site Selection in Plants and Animals

1988 • 99 citations

Accurate in vitro splicing of two pre-mRNA plant introns in a HeLa cell nuclear extract.

1986 • 95 citations

Structure of U2 snRNA genes of Arabidopsis thaliana and their expression in electroporated plant protoplasts

1988 • 84 citations

Unusual branch point selection in processing of human growth hormone pre-mRNA.

1988 • 72 citations

Splicing of plant pre-mRN As in animal systems and vice versa

1987 • 65 citations

Yeast is unable to excise foreign intervening sequences from hybrid gene transcripts.

1983 • 64 citations

A novel role for the 3' region of introns in pre-mRNA splicing of Saccharomyces cerevisiae.

1987 • 62 citations

Nuclear pre-mRNA processing in plants: distinct modes of 3'-splice-site selection in plants and animals.

1988 • 58 citations

Structure and expression of the U5 snRNA gene of Arabidopsis thaliana. Conserved upstream sequence elements in plant U-RNA genes

1988 • 54 citations

Yeast pre-messenger RNA splicing efficiency depends on critical spacing requirements between the branch point and 3′ splice site.

1986 • 53 citations

Multiple cis-acting sequence elements are required for efficient splicing of simian virus 40 small-t antigen pre-mRNA.

1988 • 51 citations

Alternative branch points are selected during splicing of a yeast pre-mRNA in mammalian and yeast extracts.

1986 • 48 citations

Effects on mRNA splicing of mutations in the 3' region of the Saccharomyces cerevisiae actin intron.

1987 • 43 citations

In vitroprocessing of a plant pre-mRNA in a HeLa cell nuclear extract

1986 • 42 citations

Structure and sequence of a UDP glucose pyrophosphorylase gene ofDictyostelium discoideum

1987 • 42 citations

Small nuclear ribonucleo-protein particles

1989 • 36 citations

Direct gene transfer to plants

1986 • 34 citations

Plant small nuclear RNAs. III. The complete primary and secondary structure of broad bean U2 RNA: phylogenetic and functional implications

1987 • 29 citations

Euglena gracilischloroplast DNA: the untranslated leader oftufA-ORF206 gene contains an intron

1987 • 26 citations

Impairment of yeast pre-mRNA splicing by potential secondary structure-forming sequences near the conserved branchpoint sequence

1988 • 26 citations

purification of the major UsnRNPs from broad bean nuclear extracts and characterization of their protein constituents

1989 • 25 citations

The secondary structure of a messenger RNA precursor probed with psoralen is melted in anin vitrosplicing reaction

1987 • 15 citations

Multiple introns in a conjugation-specific gene fromTetrahymena thermophila

1988 • 14 citations