Internalizing antibodies and targeted cancer therapy: direct selection from phage display libraries

Studies of the HER-2/ neu Proto-Oncogene in Human Breast and Ovarian Cancer

1989 • 6,734 citations

Humanization of an anti-p185HER2 antibody for human cancer therapy.

1992 • 1,867 citations

By-passing immunization

1991 • 1,603 citations

Making Antibodies by Phage Display Technology

1994 • 1,516 citations

Endocytosis

1997 • 1,263 citations

Optimizing liposomes for delivery of chemotherapeutic agents to solid tumors.

1999 • 1,231 citations

pH and the recycling of transferrin during receptor-mediated endocytosis.

1983 • 1,165 citations

Dimerization of the Extracellular Domain of the Human Growth Hormone Receptor by a Single Hormone Molecule

1991 • 879 citations

Rapid tumor penetration of a single-chain Fv and comparison with other immunoglobulin forms.

1992 • 824 citations

Gene delivery: A single nuclear localization signal peptide is sufficient to carry DNA to the cell nucleus

1999 • 718 citations

Internalization and processing of transferrin and the transferrin receptor in human carcinoma A431 cells.

1983 • 636 citations

Cure of Xenografted Human Carcinomas by BR96-Doxorubicin Immunoconjugates

1993 • 545 citations

Monoclonal anti-epidermal growth factor receptor antibodies which are inhibitors of epidermal growth factor binding and antagonists of epidermal growth factor binding and antagonists of epidermal growth factor-stimulated tyrosine protein kinase activity.

1984 • 467 citations

Efficient construction of a large nonimmune phage antibody library: The production of high-affinity human single-chain antibodies to protein antigens

1998 • 437 citations

Constitutive and antibody-induced internalization of prostate-specific membrane antigen.

1998 • 419 citations

Isolation of Picomolar Affinity Anti-c-erbB-2 Single-chain Fv by Molecular Evolution of the Complementarity Determining Regions in the Center of the Antibody Binding Site

1996 • 380 citations

Two EGF molecules contribute additively to stabilization of the EGFR dimer

1997 • 363 citations

Toward cell–targeting gene therapy vectors: Selection of cell–binding peptides from random peptide–presenting phage libraries

1996 • 335 citations

Eradication of established tumors by a fully human monoclonal antibody to the epidermal growth factor receptor without concomitant chemotherapy.

1999 • 329 citations

Treatment of advanced breast cancer with sterically stabilized liposomal doxorubicin: results of a multicenter phase II trial.

1997 • 299 citations

Molecular cloning of the human B cell CD20 receptor predicts a hydrophobic protein with multiple transmembrane domains.

1988 • 280 citations

Receptor-mediated and enzyme-dependent targeting of cytotoxic anticancer drugs

1999 • 273 citations

Fc receptor isoforms exhibit distinct abilities for coated pit localization as a result of cytoplasmic domain heterogeneity

1989 • 259 citations

Development of anti-p185HER2 immunoliposomes for cancer therapy.

1995 • 258 citations

Endocytosis and degradation of monoclonal antibodies targeting human B-cell malignancies.

1989 • 255 citations

Monoclonal antibody against epidermal growth factor receptor is internalized without stimulating receptor phosphorylation.

1986 • 250 citations

Expression of mutated epidermal growth factor receptor by non-small cell lung carcinomas.

1993 • 246 citations

Increased affinity leads to improved selective tumor delivery of single-chain Fv antibodies.

1998 • 234 citations

Rapid selection of cell subpopulation-specific human monoclonal antibodies from a synthetic phage antibody library.

1995 • 218 citations

Prolonged in vivo tumour retention of a human diabody targeting the extracellular domain of human HER2/neu

1998 • 209 citations

Anti-melanoma antibodies from melanoma patients immunized with genetically modified autologous tumor cells: selection of specific antibodies from single-chain Fv fusion phage libraries.

1995 • 207 citations

Monoclonal antibodies against receptor for epidermal growth factor induce early and delayed effects of epidermal growth factor.

1981 • 191 citations

Selection of tumor-specific internalizing human antibodies from phage libraries

2000 • 190 citations

Targeting of stealth liposomes to erbB-2 (Her/2) receptor: in vitro and in vivo studies

1996 • 179 citations

A subclass of tumor-inhibitory monoclonal antibodies to ErbB-2/HER2 blocks crosstalk with growth factor receptors

1997 • 178 citations

Cell binding and internalization by filamentous phage displaying a cyclic Arg-Gly-Asp-containing peptide.

1994 • 164 citations

Characterization of an anti-p185HER2 monoclonal antibody that stimulates receptor function and inhibits tumor cell growth.

1991 • 163 citations

In vitro and in vivo characterization of a human anti-c-erbB-2 single-chain Fv isolated from a filamentous phage antibody library

1995 • 160 citations

Suppression and promotion of tumor growth by monoclonal antibodies to ErbB-2 differentially correlate with cellular uptake.

1995 • 149 citations

Toward Selection of Internalizing Antibodies from Phage Libraries

1999 • 145 citations

A recombinant antibody with the antigen-specific, major histocompatibility complex-restricted specificity of T cells.

1996 • 131 citations

Target Cell-specific DNA Transfer Mediated by a Chimeric Multidomain Protein

1996 • 125 citations

Anti-HER2 immunoliposomes for targeted therapy of human tumors

1997 • 122 citations

Selection‐dominant and nonaccessible epitopes on cell‐surface receptors revealed by cell‐panning with a large phage antibody library

1999 • 115 citations

Recombinant Fv immunotoxins and Fv fragments as novel agents for cancer therapy and diagnosis

1998 • 115 citations

Multivalent Fvs: characterization of single-chain Fv oligomers and preparation of a bispecific Fv

1994 • 113 citations

Homodimerization of tumor-reactive monoclonal antibodies markedly increases their ability to induce growth arrest or apoptosis of tumor cells

1997 • 112 citations

Selection of monoclonal antibodies which induce internalization and phosphorylation of P185^HER2 and growth inhibition of cells with HER2/neu gene amplification

1991 • 109 citations

Agonistic antibodies stimulate the kinase encoded by the neu protooncogene in living cells but the oncogenic mutant is constitutively active.

1990 • 105 citations

Processing of antibody-radioisotope conjugates after binding to the surface of tumor cells

1994 • 99 citations

Antibody-induced dimerization activates the epidermal growth factor receptor tyrosine kinase

1991 • 96 citations

Ricin A-chain containing immunotoxins directed against different epitopes on the CD2 molecule differ in their ability to kill normal and malignant T cells.

1988 • 95 citations

Phage Libraries — A New Route to Clinically Useful Antibodies

1996 • 92 citations

Enhanced tumor localization and in vivo stability of a monoclonal antibody radioiodinated using N-succinimidyl 3-(tri-n-butylstannyl)benzoate.

1989 • 85 citations

Clinical status and optimal use of rituximab for B-cell lymphomas.

1998 • 84 citations

Influence of Membrane-Active Peptides on Lipospermine/DNA Complex Mediated Gene Transfer

1997 • 81 citations

Growth Inhibition of CD20‐positive B Lymphoma Cell Lines by IDEC‐C2B8 Anti‐CD20 Monoclonal Antibody

1998 • 78 citations

Modulation of transferrin receptor expression and function by anti-transferrin receptor antibodies and antibody fragments

1989 • 75 citations

Evaluation of four CD22 antibodies as ricin a chain‐containing immunotoxins for the in vivo therapy of human B‐cell leukemias and lymphomas

1988 • 71 citations

Cellular Trafficking and Cytotoxicity of Anti-Cd19-Targeted Liposomal Doxorubicin in B Lymphoma Cells

1999 • 71 citations

Therapeutic efficacy and dose-limiting toxicity of auger-electronvs. beta emitters in radioimmunotherapy with internalizing antibodies: Evaluation of125I-vs.131I-labeled CO17-1A in a human colorectal cancer model

1998 • 60 citations

Cytotoxicity of gelonin and its conjugates with antibodies is determined by the extent of their endocytosis

1989 • 60 citations

Role of ligand in antibody-directed endocytosis of liposomes by human T-leukemia cells.

1989 • 59 citations

Antibody-Targeted Drugs for the Therapy of Cancer

1994 • 58 citations

Design of a genetic immunotoxin to eliminate toxin immunogenicity.

1995 • 48 citations

Drug transfer into lymphoblasts mediated by liposomes bound to distinct sites on H-2 encoded I-A, I-E, and K molecules.

1982 • 42 citations

Charge-modified single chain antibody constructs of monoclonal antibody CC49: generation, characterization, pharmacokinetics, and biodistribution analysis

1999 • 40 citations

CD4 and CD7 molecules as targets for drug delivery from antibody bearing liposomes

1991 • 38 citations

Biotechnology Update

1999 • 37 citations

Comparison of the biological properties of two anti-mucin-1 antibodies prepared for imaging and therapy

1997 • 35 citations

Antibody-directed targeting of liposomes to human cell lines: role of binding and internalization on growth inhibition.

1987 • 32 citations

The performance of e23(Fv)PEs, recombinant toxins targeting the erbB-2 protein

1996 • 20 citations

Antigen density on target cells determines the immunosuppressive potential of rat IgG_2b monoclonal antibodies

1990 • 17 citations

Recombinant Immunotoxin Containing a Disulfide-Stabilized Fv Directed at erbB2 That Does Not Require Proteolytic Activation

1996 • 15 citations

Antibody-induced activation of p185HER2 in the human lung adenocarcinoma cell line Calu-3 requires bivalency

1993 • 14 citations

Antibody transport and internalization into tumours.

1990 • 14 citations

Demonstration of monoclonal anti-carcinoembryonic antigen (CEA) antibody internalization by electron microscopy, western blotting and radioimmunoassay.

1992 • 13 citations

Targeting behavior of rat monoclonal IgG antibodiesin vivo: role of antibody isotype, specificity and the target cell antigen density

1991 • 13 citations

Cell Membrane Antigen-Antibody Complex Dissociation by the Widely Used Glycine-Hcl Method: An Unreliable Procedure for Studying Antibody Internalization

1993 • 12 citations

Immunoscintigraphy of small-cell lung cancer xenografts with anti neural cell adhesion molecule monoclonal antibody, 123C3: improvement of tumour uptake by internalisation

1996 • 11 citations

Antitumour activity of an immunoconjugate composed of anti-human astrocytoma monoclonal antibody and neocarzinostatin

1993 • 9 citations

Differential immunofluorescent staining of polymorphonuclear leucocytes for discriminating between surface bound and internalised immunoglobulin

1982 • 5 citations

Receptor-mediated endocytosis of epidermal growth factor by hepatocytes in the perfused rat liver.

1985 • 2 citations