Ubiquitin-assisted dissection of protein transport across membranes.

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

1989 • 8,705 citations

A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids

1984 • 3,959 citations

In Vivo Half-Life of a Protein Is a Function of Its Amino-Terminal Residue

1986 • 1,942 citations

Structure of ubiquitin refined at 1.8 Å resolution

1987 • 1,682 citations

Intracellular protein topogenesis

1980 • 1,378 citations

Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method.

1988 • 983 citations

Polypeptide chain binding proteins: Catalysts of protein folding and related processes in cells

1989 • 884 citations

Translocation of proteins across the endoplasmic reticulum III. Signal recognition protein (SRP) causes signal sequence-dependent and site-specific arrest of chain elongation that is released by microsomal membranes.

1981 • 807 citations

The tails of ubiquitin precursors are ribosomal proteins whose fusion to ubiquitin facilitates ribosome biogenesis

1989 • 711 citations

A Genetic Approach to Analyzing Membrane Protein Topology

1986 • 610 citations

The yeast STE6 gene encodes a homologue of the mammalian multidrug resistance P-glycoprotein

1989 • 604 citations

Structure of a yeast pheromone gene (MFα): A putative α-factor precursor contains four tandem copies of mature α-factor

1982 • 587 citations

THE UBIQUITIN-CONJUGATION SYSTEM

1992 • 561 citations

The N-End Rule in Bacteria

1991 • 536 citations

Mechanism of Protein Translocation Across the Endoplasmic Reticulum Membrane

1986 • 509 citations

The yeast ubiquitin genes: a family of natural gene fusions.

1987 • 500 citations

Protein Sorting to Mitochondria: Evolutionary Conservations of Folding and Assembly

1990 • 494 citations

Transcending the impenetrable: How proteins come to terms with membranes

1988 • 471 citations

The N-end rule

1992 • 464 citations

[76] β-d-Fructofuranoside fructohydrolase from yeast

1975 • 455 citations

Saccharomyces cerevisiae STE6 gene product: a novel pathway for protein export in eukaryotic cells.

1989 • 443 citations

THE ENZYMOLOGY OF PROTEIN TRANSLOCATION ACROSS THE Escherichia coli PLASMA MEMBRANE

1991 • 442 citations

A yeast mutant defective at an early stage in import of secretory protein precursors into the endoplasmic reticulum.

1987 • 418 citations

Signal sequences

1989 • 417 citations

The recognition component of the N-end rule pathway.

1990 • 388 citations

An efficient transformation procedure enabling long‐term storage of competent cells of various yeast genera

1991 • 379 citations

Transport of Proteins Across the Endoplasmic Reticulum Membrane

1992 • 366 citations

Heat-Inducible Degron: a Method for Constructing Temperature-Sensitive Mutants

1994 • 349 citations

Protein sorting in yeast: The localization determinant of yeast vacuolar carboxypeptidase Y resides in the propeptide

1987 • 329 citations

Purification of a RAS-Responsive Adenylyl Cyclase Complex from Saccharomyces cerevisiae by Use of an Epitope Addition Method

1988 • 328 citations

The Structure and Insertion of Integral Proteins in Membranes

1990 • 304 citations

The signal recognition particle in S. cerevisiae

1991 • 299 citations

The N-end rule is mediated by the UBC2(RAD6) ubiquitin-conjugating enzyme.

1991 • 268 citations

Ubiquitin as a degradation signal.

1992 • 261 citations

Ubiquitin-specific proteases of Saccharomyces cerevisiae. Cloning of UBP2 and UBP3, and functional analysis of the UBP gene family

1992 • 250 citations

A Kinetic Partitioning Model of Selective Binding of Nonnative Proteins by the Bacterial Chaperone SecB

1991 • 225 citations

Nucleotide sequence of The yeastSUC2gene for invertase

1983 • 225 citations

Unity in Function in the Absence of Consensus in Sequence: Role of Leader Peptides in Export

1989 • 222 citations

Early hydrogen-bonding events in the folding reaction of ubiquitin.

1992 • 220 citations

Signal recognition particle mediates a transient elongation arrest of preprolactin in reticulocyte lysate.

1989 • 148 citations

Pause transfer: A topogenic sequence in apolipoprotein B mediates stopping and restarting of translocation

1992 • 116 citations

Saccharomyces cerevisiae and Schizosaccharomyces pombe contain a homologue to the 54-kD subunit of the signal recognition particle that in S. cerevisiae is essential for growth.

1989 • 115 citations

A 30-residue-long "export initiation domain" adjacent to the signal sequence is critical for protein translocation across the inner membrane of Escherichia coli.

1991 • 95 citations

The protein import machinery of mitochondria

1993 • 89 citations

The N-end rule in Escherichia coli: cloning and analysis of the leucyl, phenylalanyl-tRNA-protein transferase gene aat

1993 • 87 citations

Genetic and biochemical evaluation of eucaryotic membrane protein topology: multiple transmembrane domains of Saccharomyces cerevisiae 3-hydroxy-3-methylglutaryl coenzyme A reductase.

1990 • 71 citations

[47] Analysis of glycoproteins from Saccharomyces cerevisiae

1991 • 64 citations

Cotranslational and Posttranslational Protein Translocation in Prokaryotic Systems

1986 • 53 citations

A New Type of Fusion Analysis Applicable to Many Organisms: Protein Fusions to the URA3 Gene of Yeast

1987 • 44 citations

Discrete nascent chain lengths are required for the insertion of presecretory proteins into microsomal membranes.

1993 • 40 citations

The dynamics of assembly of a cytoplasmic membrane protein in Escherichia coli.

1992 • 36 citations

Efficiency and diversity of protein localization by random signal sequences.

1990 • 28 citations

C-terminal sequences can inhibit the insertion of membrane proteins into the endoplasmic reticulum of Saccharomyces cerevisiae.

1992 • 26 citations

Cassette Mutagenic Analysis of the Yeast Invertase Signal Peptide: Effects on Protein Translocation

1989 • 24 citations

Genetic and Biochemical Evaluation of Eucaryotic Membrane Protein Topology: Multiple Transmembrane Domains of Saccharomyces cerevisiae 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase

1990 • 21 citations

Protein translocation into the endoplasmic reticulum: a light at the end of the tunnel

1991 • 18 citations

Efficiency and Diversity of Protein Localization by Random Signal Sequences

1990 • 6 citations

C-Terminal Sequences Can Inhibit the Insertion of Membrane Proteins into the Endoplasmic Reticulum of Saccharomyces cerevisiae

1992 • 5 citations