[39] Glucocorticoid receptor thiols andsteroid-binding activity

Primary structure and expression of a functional human glucocorticoid receptor cDNA

1985 • 1,851 citations

Genetic complementation of a glucocorticoid receptor deficiency by expression of cloned receptor cDNA

1986 • 722 citations

The role of heat shock proteins in regulating the function, folding, and trafficking of the glucocorticoid receptor.

1993 • 652 citations

A Specific and Sensitive Assay for Disulfides

1968 • 446 citations

Evidence That the 90-kDa Heat Shock Protein Is Necessary for the Steroid Binding Conformation of the L Cell Glucocorticoid Receptor

1989 • 394 citations

The mouse glucocorticoid receptor: mapping of functional domains by cloning, sequencing and expression of wild-type and mutant receptor proteins.

1986 • 392 citations

Evidence that the endogenous heat-stable glucocorticoid receptor-activating factor is thioredoxin.

1983 • 309 citations

Raf exists in a native heterocomplex with hsp90 and p50 that can be reconstituted in a cell-free system.

1993 • 302 citations

Separation of the hormone- and DNA-binding sites of the hepatic glucocorticoid receptor by means of proteolysis.

1978 • 258 citations

Glucocorticoid-binding Proteins of Rat Liver Cytosol

1972 • 206 citations

Characterization of the purified activated glucocorticoid receptor from rat liver cytosol.

1984 • 186 citations

Activation of thymocyte glucocorticoid receptors to the steroid binding form. The roles of reduction agents, ATP, and heat-stable factors.

1979 • 171 citations

Proof that hsp70 is required for assembly of the glucocorticoid receptor into a heterocomplex with hsp90.

1994 • 169 citations

Localization of the 90-kDa heat shock protein-binding site within the hormone-binding domain of the glucocorticoid receptor by peptide competition

1991 • 165 citations

Proof that the endogenous, heat-stable glucocorticoid receptor-activating factor is thioredoxin.

1985 • 158 citations

Effects of Molybdate and Endogenous Inhibitors on Steroid-Receptor Inactivation, Transformation, and Translocation

1984 • 153 citations

ATP induces a conformational change of the 90-kDa heat shock protein (hsp90).

1993 • 133 citations

Identification of cysteine 656 as the amino acid of hepatoma tissue culture cell glucocorticoid receptors that is covalently labeled by dexamethasone 21-mesylate.

1987 • 125 citations

Identification of hormone-interacting amino acid residues within the steroid-binding domain of the glucocorticoid receptor in relation to other steroid hormone receptors.

1988 • 121 citations

Specific covalent modification of thiols: Applications in the study of enzymes and other biomolecules

1979 • 118 citations

Arsenite and cadmium(II) as probes of glucocorticoid receptor structure and function.

1990 • 118 citations

Nature of steroid-glucocorticoid receptor interactions: thermodynamic analysis of the binding reaction

1978 • 105 citations

Affinity labeling of the rat liver glucocorticoid receptor with dexamethasone 21-mesylate. Identification of covalently labeled receptor by immunochemical methods.

1981 • 101 citations

The Role of Sulfhydryl Groups in the Binding of Glucocorticoids by Cytoplasmic Receptors of Lung and Other Mammalian Tissues¹

1977 • 97 citations

Creation of “super” glucocorticoid receptors by point mutations in the steroid binding domain.

1991 • 94 citations

The involvement of receptor sulphydryl groups in the binding of steroids to the cytoplasmic glucocorticoid receptor from rat thymus

1975 • 93 citations

Role of cysteines 640, 656, and 661 in steroid binding to rat glucocorticoid receptors.

1992 • 77 citations

Steroid Binding Activity Is Retained in a 16-kDa Fragment of the Steroid Binding Domain of Rat Glucocorticoid Receptors

1989 • 75 citations

Structurally based, selective interaction of arsenite with steroid receptors.

1990 • 75 citations

Reversible dissociation of steroid hormone x receptor complexes by mercurial reagents.

1980 • 73 citations

Sulfhydryl-modifying reagents reversibly inhibit binding of glucocorticoid-receptor complexes to DNA-cellulose

1984 • 71 citations

The role of sulfhydryl groups in permitting transformation and DNA binding of the glucocorticoid receptor.

1987 • 64 citations

Characteristics of an endogenous glucocorticoid receptor stabilizing factor.

1982 • 62 citations

Inhibition of coupling factor B activity by cadmium ion, arsenite-2,3-dimercaptopropanol, and phenylarsine oxide, and preferential reactivation by dithiols.

1981 • 58 citations

Reactivation of thymocyte glucocorticoid receptors in a cell-free system.

1977 • 55 citations

Anti-inflammatory pyrazolo-steroids: Potent glucocorticoids containing bulky a-ring substituents and no C3-carbonyl

1979 • 52 citations

17β- Carboxamide steroids are a new class of glucocorticoid antagonists

1979 • 51 citations

Steroid binding to hepatoma tissue culture cell glucocorticoid receptors involves at least two sulfhydryl groups.

1988 • 49 citations

The protein-protein complex between pp60v-src and hsp90 is stabilized by molybdate, vanadate, tungstate, and an endogenous cytosolic metal.

1992 • 47 citations

Role of chemical reagents in the activation of rat hepatic glucocorticoid-receptor complex.

1980 • 46 citations

Identification of cysteine-644 as the covalent site of attachment of dexamethasone 21-mesylate to murine glucocorticoid receptors in WEHI-7 cells

1988 • 46 citations

Characterization of the rat liver glucocorticoid receptor purified by DNA-cellulose and ligand affinity chromatography.

1984 • 45 citations

Hydrogen peroxide stabilizes the steroid-binding state of rat liver glucocorticoid receptors by promoting disulfide bond formation

1988 • 41 citations

Identification of two cysteines closely positioned in the ligand-binding pocket of the human estrogen receptor: roles in ligand binding and transcriptional activation.

1992 • 41 citations

Selective covalent labeling of cysteines in bovine serum albumin and in hepatoma tissue culture cell glucocorticoid receptors by dexamethasone 21-mesylate.

1987 • 38 citations

Differential effects of the reversible thiol-reactive agents arsenite and methyl methanethiosulfonate on steroid binding by the glucocorticoid receptor

1993 • 38 citations

The possible role of disulfide bond reduction in transformation of the 10 S androgen receptor.

1986 • 36 citations

Development of an [3H] glucocorticoid exchange assay in rat liver cytosol

1981 • 34 citations

Mammalian Progesterone Receptor Shows Differential Sensitivity to Sulfhydryl Group Modifying Agents When Bound to Agonist and Antagonist Ligands

1989 • 34 citations

Association of heat shock protein 90 with the 16 kDa steroid binding core fragment of rat glucocorticoid receptors

1991 • 28 citations

Selective molybdate-directed covalent modification of sulfhydryl groups in the steroid-binding versus the DNA-binding domain of the glucocorticoid receptor.

1990 • 25 citations

Localization of the Vicinal Dithiols Involved in Steroid Binding to the Rat Glucocorticoid Receptor

1990 • 23 citations

Interaction of antiglucocorticoid RU 486 with rat kidney glucocorticoid receptor

1988 • 21 citations

Identification of two cysteines closely positioned in the ligand- binding pocket of the human estrogen receptor: roles in ligand binding and transcriptional activation

1992 • 20 citations

Absence of intramolecular disulfides in the structure and function of native rat glucocorticoid receptors.

1994 • 19 citations

Metal oxyanion stabilization of the rat glucocorticoid receptor is independent of thiols.

1994 • 18 citations

Titratable effects of p-chloromercuriphenyl sulfonate, a thiol-attacking reagent, on glucocorticoid receptor binding

1983 • 17 citations

Inhibition of glucocorticoid receptor transformation, subunit dissociation, and temperature-dependent inactivation by various N-substituted maleimides

1988 • 17 citations

The hormone-binding role of 2 cysteines near the C terminus of the mouse glucocorticoid receptor.

1994 • 14 citations

Inactivation of unbound rat liver glucocorticoid receptor by N-alkylmaleimides at sub-zero temperatures

1984 • 12 citations

Interaction of rat liver glucocorticoid receptor with a newly synthesized antisteroid ZK98299

1991 • 8 citations

Binding of RU486 and deacylcortivazol to the glucocorticoid receptor is insensitive to sulfhydryl-modifying agents

1993 • 6 citations