Membrane insertion of the mannitol permease of Escherichia coli occurs under conditions of impaired SecA function.

ACETYLORNITHINASE OF ESCHERICHIA COLI: PARTIAL PURIFICATION AND SOME PROPERTIES

1956 • 4,156 citations

[6] Quantitation of protein

1990 • 1,401 citations

Intracellular protein topogenesis

1980 • 1,378 citations

The distribution of positively charged residues in bacterial inner membrane proteins correlates with the trans-membrane topology

1986 • 861 citations

Control of topology and mode of assembly of a polytopic membrane protein by positively charged residues

1989 • 587 citations

The binding cascade of SecB to SecA to SecYE mediates preprotein targeting to the E. coli plasma membrane

1990 • 561 citations

The ATPase activity of secA is regulated by acidic phospholipids, secY, and the leader and mature domains of precursor proteins

1990 • 551 citations

ΔμH+ and ATP function at different steps of the catalytic cycle of preprotein translocase

1991 • 456 citations

THE ENZYMOLOGY OF PROTEIN TRANSLOCATION ACROSS THE Escherichia coli PLASMA MEMBRANE

1991 • 442 citations

Regulation of a membrane component required for protein secretion in escherichia coli

1982 • 356 citations

Translocation of secretory proteins across the microsomal membrane occurs through an environment accessible to aqueous perturbants

1985 • 277 citations

Evidence for specificity at an early step in protein export in Escherichia coli

1985 • 272 citations

Proposed uniform nomenclature for the proteins and protein domains of the bacterial phosphoenolpyruvate: sugar phosphotransferase system

1992 • 259 citations

lac permease of Escherichia coli: topology and sequence elements promoting membrane insertion.

1990 • 248 citations

SecA protein is required for secretory protein translocation into E. coli membrane vesicles

1988 • 239 citations

Topology analysis of the SecY protein, an integral membrane protein involved in protein export in Escherichia coli.

1987 • 223 citations

Effects of two sec genes on protein assembly into the plasma membrane of Escherichia coli.

1985 • 217 citations

A defined mutation in the protein export gene within the spc ribosomal protein operon of Escherichia coli: isolation and characterization of a new temperature-sensitive secY mutant.

1984 • 216 citations

SecA protein, a peripheral protein of the Escherichia coli plasma membrane, is essential for the functional binding and translocation of proOmpA.

1989 • 206 citations

Mannitol-specific enzyme II of the bacterial phosphotransferase system. III. The nucleotide sequence of the permease gene.

1983 • 177 citations

Genetic analysis of the membrane insertion and topology of MalF, a cytoplasmic membrane protein of Escherichia coli

1988 • 175 citations

In vitro translocation of bacterial proteins across the plasma membrane of Escherichia coli.

1984 • 174 citations

Thesec andprl genes ofEscherichia coli

1990 • 174 citations

Insertion of proteins into bacterial membranes: mechanism, characteristics, and comparisons with the eucaryotic process

1989 • 167 citations

Sugar transport. Properties of mutant bacteria defective in proteins of the phosphoenolpyruvate: sugar phosphotransferase system.

1976 • 158 citations

Positively charged residues are important determinants of membrane protein topology

1990 • 132 citations

M13 procoat inserts into liposomes in the absence of other membrane proteins.

1985 • 121 citations

The nature of information, required for export and sorting, present within the outer membrane protein OmpA of Escherichia coli K-12.

1985 • 114 citations

SecB functions as a cytosolic signal recognition factor for protein export in E. coli

1989 • 107 citations

Sugar permeases of the bacterial phosphoenolpyruvate‐dependent phosphotransferase system: sequence comparisons

1988 • 102 citations

Protein export in Escherichia coli requires a soluble activity.

1984 • 100 citations

The cytoplasmic carboxy terminus of M13 procoat is required for the membrane insertion of its central domain

1986 • 92 citations

In vivo analysis of integration of membrane proteins in Escherichia coli

1991 • 92 citations

SecA protein: Autoregulated initiator of secretory precursor protein translocation across theE. coli plasma membrane

1990 • 87 citations

Deletion mutants of the Escherichia coli K-12 mannitol permease: dissection of transport-phosphorylation, phospho-exchange, and mannitol-binding activities

1989 • 71 citations

Efficient translocation of positively charged residues of M13 procoat protein across the membrane excludes electrophoresis as the primary force for membrane insertion.

1990 • 63 citations

SecA suppresses the temperature-sensitive SecY24 defect in protein translocation in Escherichia coli membrane vesicles.

1988 • 62 citations

Mannitol-specific enzyme II of the bacterial phosphotransferase system. I. Properties of the purified permease.

1983 • 61 citations

Export of Escherichia coli Alkaline Phosphatase Attached to an Integral Membrane Protein, SecY

1989 • 60 citations

The Tsr chemosensory transducer of Escherichia coli assembles into the cytoplasmic membrane via a SecA-dependent process.

1988 • 58 citations

Biochemical evidence for the secY24 defect in Escherichia coli protein translocation and its suppression by soluble cytoplasmic factors.

1987 • 56 citations

Membrane insertion of the Escherichia coli MalF protein in cells with impaired secretion machinery.

1991 • 47 citations

The internal signal sequence of Escherichia coli leader peptidase is necessary, but not sufficient, for its rapid membrane assembly.

1987 • 47 citations

The N-terminal region of Escherichia coli lactose permease mediates membrane contact of the nascent polypeptide chain

1987 • 43 citations

Skp is a periplasmic Escherichia coli protein requiring SecA and SecY for export

1991 • 42 citations

Membrane disposition of the Escherichia coli mannitol permease: identification of membrane-bound and cytoplasmic domains

1986 • 40 citations

Plasmid-directed synthesis of enzymes required for D-mannitol transport and utilization in Escherichia coli.

1981 • 39 citations

In vitro membrane assembly of a polytopic, transmembrane protein results in an enzymatically active conformation.

1989 • 39 citations

Use of cloned mtl genes of Escherichia coli to introduce mtl deletion mutations into the chromosome

1983 • 37 citations

Determinants of membrane-targeting and transmembrane translocation during bacterial protein export.

1990 • 35 citations

Both a short hydrophobic domain and a carboxyl-terminal hydrophilic region are important for signal function in the Escherichia coli leader peptidase

1989 • 30 citations

The intramembrane topography of the mannitol-specific enzyme II of the Escherichia coli phosphotransferase system.

1983 • 30 citations

Embedded or not? hydrophobic sequences and membranes

1990 • 27 citations

Bacterial proteins with N-terminal leader sequences resembling mitochondrial targeting sequences of eukaryotes

1988 • 27 citations

Truncated forms of Escherichia coli lactose permease: models for study of biosynthesis and membrane insertion

1988 • 27 citations

Insertion of the mannitol permease into the membrane of Escherichia coli. Possible involvement of an N-terminal amphiphilic sequence.

1991 • 27 citations

Does secA mediate coupling between secretion and translation in Escherichia coli?

1986 • 25 citations

Development of a cell-free system to study the membrane assembly of photosynthetic proteins of Rhodobacter capsulatus.

1990 • 25 citations

Structure, function, and biogenesis of SecY, an integral membrane protein involved in protein export

1990 • 21 citations

Membrane Assembly of Lactose Permease of Escherichia coli

1992 • 15 citations

Membrane biogenesis in Escherichia coli: effects of a secA mutation

1989 • 15 citations

Protein translocationin vitro: Biochemical characterization of genetically defined translocation components

1990 • 15 citations

Topological analysis of the amino‐terminal region of lactose permease using the Escherichia coli outer membrane protein, OmpA, as a marker

1989 • 3 citations