Chapter 3 The Use of Genetic Techniques to Analyze Protein Export in Escherichia coli

Empirical Predictions of Protein Conformation

1978 • 3,167 citations

Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu

1976 • 1,895 citations

The Release of Enzymes from Escherichia coli by Osmotic Shock and during the Formation of Spheroplasts

1965 • 1,490 citations

A putative signal peptidase recognition site and sequence in eukaryotic and prokaryotic signal peptides

1983 • 1,143 citations

In vitro gene fusions that join an enzymatically active beta-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals

1980 • 1,052 citations

The spontaneous insertion of proteins into and across membranes: The helical hairpin hypothesis

1981 • 868 citations

Translocation of proteins across the endoplasmic reticulum III. Signal recognition protein (SRP) causes signal sequence-dependent and site-specific arrest of chain elongation that is released by microsomal membranes.

1981 • 807 citations

Secretory protein translocation across membranes—the role of the ‘docking protein’

1982 • 648 citations

Gene amplification and analysis

1986 • 517 citations

E. coli mutant pleiotropically defective in the export of secreted proteins

1981 • 512 citations

Purification of a membrane-associated protein complex required for protein translocation across the endoplasmic reticulum.

1980 • 502 citations

Isolation of the Bacteriophage Lambda Receptor from Escherichia coli

1973 • 433 citations

Suppressor mutations that restore export of a protein with a defective signal sequence

1981 • 382 citations

Trans‐membrane Translocation of Proteins

1979 • 364 citations

Protein localization in E. coli: Is there a common step in the secretion of periplasmic and outer-membrane proteins?

1981 • 359 citations

Regulation of a membrane component required for protein secretion in escherichia coli

1982 • 356 citations

A role for mRNA secondary structure in the control of translation initiation

1982 • 344 citations

Mechanism of Incorporation of Cell Envelope Proteins in Escherichia Coli

1982 • 344 citations

Maltose transport in Escherichia coli K-12: involvement of the bacteriophage lambda receptor

1975 • 317 citations

Amino acid sequence for the peptide extension on the prolipoprotein of the Escherichia coli outer membrane.

1977 • 286 citations

Mechanisms of protein localization

1983 • 282 citations

Mutations that alter the signal sequence of alkaline phosphatase in Escherichia coli

1983 • 281 citations

Mutations in a new gene, secB, cause defective protein localization in Escherichia coli

1983 • 270 citations

Mutations which alter the function of the signal sequence of the maltose binding protein of Escherichia coli

1980 • 268 citations

Use of gene fusion to study secretion of maltose-binding protein into Escherichia coli periplasm

1979 • 267 citations

Thespcrtbosomal protein operon ofEschenchia coli:sequence and cotranscriptlon of the rlbosomal protein genes and a protein export gene

1983 • 258 citations

Bacterial outer membranes: Biogenesis and functions

1979 • 254 citations

Translocation of domains of nascent periplasmic proteins across the cytoplasmic membrane is independent of elongation

1983 • 234 citations

Role of positive charge on the amino-terminal region of the signal peptide in protein secretion across the membrane.

1982 • 234 citations

Improved methods for maximizing expression of a cloned gene: a bacterium that synthesizes rabbit β-globin

1980 • 227 citations

A temperature-sensitive mutant of E. coli exhibiting slow processing of exported proteins

1983 • 215 citations

Different exported proteins in E. coli show differences in the temporal mode of processing in vivo

1981 • 203 citations

Post-translational modification and processing of Escherichia coli prolipoprotein in vitro.

1982 • 201 citations

Sequence analysis of mutations that prevent export of λ receptor, an Escherichia coli outer membrane protein

1980 • 195 citations

Synthesis of Exported Proteins by Membrane-Bound Polysomes from Escherichia coli

1977 • 177 citations

Mutations affecting localization of an Escherichia coli outer membrane protein, the bacteriophage λ receptor

1980 • 153 citations

Modern cell biology

1983 • 153 citations

Importance of secondary structure in the signal sequence for protein secretion.

1983 • 152 citations

A signal sequence is not sufficient to lead β-galactosidase out of the cytoplasm

1980 • 149 citations

Bacteria mature preproinsulin to proinsulin.

1980 • 143 citations

Maltose and lactose transport in Escherichia coli Examples of two different types of concentrative transport systems

1983 • 143 citations

Identification of a new gene (secA) and gene product involved in the secretion of envelope proteins in Escherichia coli

1982 • 134 citations

Escherichia coli mutants accumulating the precursor of a secreted protein in the cytoplasm

1979 • 129 citations

A mechanism of protein localization: the signal hypothesis and bacteria.

1980 • 127 citations

A previously unidentified gene in the spc operon of Escherichia coli K12 specifies a component of the protein export machinery

1982 • 126 citations

Localization and processing of outer membrane and periplasmic proteins in Escherichia coli strains harboring export-specific suppressor mutations.

1982 • 124 citations

Isolation of the Escherichia coli leader peptidase gene and effects of leader peptidase overproduction in vivo.

1981 • 119 citations

A bacterial secretory protein requires signal recognition particle for translocation across mammalian endoplasmic reticulum.

1982 • 118 citations

Demonstration by a Novel Genetic Technique That Leader Peptidase Is an Essential Enzyme of Escherichia coli

1983 • 116 citations

Intragenic suppressor mutations that restore export of maltose binding protein with a truncated signal peptide

1984 • 111 citations

A Genetic Method for Determining the Order of Events in a Biological Pathway

1973 • 103 citations

Mechanisms of protein localization.

1983 • 100 citations

Chicken ovalbumin is synthesized and secreted by Escherichia coli.

1978 • 100 citations

GENETIC APPROACHES TO THE ANALYSIS OF MICROBIAL DEVELOPMENT

1982 • 88 citations

Sequence information within the lamB gene is required for proper routing of the bacteriophage λ receptor protein to the outer membrane of Escherichia coli K-12

1982 • 79 citations

Evidence for a coupling of synthesis and export of an outer membrane protein in Escherichia coli.

1983 • 78 citations

Information within the mature LamB protein necessary for localization to the outer membrane of E coli K12

1983 • 75 citations

Proper interaction between at least two components is required for efficient export of proteins to the Escherichia coli cell envelope

1985 • 74 citations

The synthesis of export-defective proteins can interfere with normal protein export in Escherichia coli.

1984 • 73 citations

Intragenic regions required for LamB export.

1984 • 70 citations

Role of the mature protein sequence of maltose-binding protein in its secretion across the E. coli cytoplasmic membrane

1981 • 69 citations

Molecular components of the signal sequence that function in the initiation of protein export.

1982 • 61 citations

Amino acid sequence homology among the major outer membrane proteins of Escherichia coli.

1984 • 56 citations

Interaction of the cheC and cheZ gene products is required for chemotactic behavior in Escherichia coli.

1979 • 53 citations

The product of gene secC is involved in the synthesis of exported proteins in E. coli

1984 • 52 citations

Signal sequence mutations disrupt feedback between secretion of an exported protein and its synthesis in E. coli

1984 • 46 citations

Dependence of secretion and assembly of type 1 fimbrial subunits of Escherichia coli on normal protein export

1984 • 41 citations

Sites within gene lacZ of Escherichia coli for formation of active hybrid beta-galactosidase molecules

1979 • 40 citations

Export and processing of MalE-LacZ hybrid proteins in Escherichia coli

1984 • 35 citations

Cotranslational secretion of diphtheria toxin and alkaline phosphatase in vitro: involvement of membrane protein(s)

1980 • 35 citations

Purification of the precursor form of maltose-binding protein, a periplasmic protein of Escherichia coli.

1982 • 34 citations

Mapping of the lipoprotein signal peptidase gene (lsp)

1984 • 30 citations

[2] Isolation and characterization of mutants of Escherichia coli K12 affected in protein localization

1983 • 20 citations

Insertion of a MalE β-Galactosidase Fusion Protein into the Envelope of Escherichia coli Disrupts Biogenesis of Outer Membrane Proteins and Processing of Inner Membrane Proteins

1982 • 17 citations

Functional implications of secondary structure analysis of wild type and mutant bacterial signal peptides.

1981 • 17 citations

Both linked and unlinked mutations can alter the intracellular site of synthesis of exported proteins of Escherichia coli

1985 • 16 citations